Development, Validation and Pharmacokinetic Application of a Simple and Robust RP-HPLC Method for Quantitation of Raloxifene in Rat Plasma
SUMMARY. A simple and sensitive HPLC method has been developed and validated for the quantification of raloxifene in rat plasma. Liquid- liquid extraction procedure was employed for extracting raloxifene from rat plasma sample. Separation of raloxifene was achieved through a RP- C18 column with a...
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College of Pharmacists of Buenos Aires Province, Argentina
2017
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| Online Access: | http://umpir.ump.edu.my/id/eprint/19791/ http://umpir.ump.edu.my/id/eprint/19791/ http://umpir.ump.edu.my/id/eprint/19791/ http://umpir.ump.edu.my/id/eprint/19791/1/ftech-2017-mahmood-Development%2C%20Validation%20and%20Pharmacokinetic%20Application1.pdf |
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ump-197912018-02-05T02:31:17Z http://umpir.ump.edu.my/id/eprint/19791/ Development, Validation and Pharmacokinetic Application of a Simple and Robust RP-HPLC Method for Quantitation of Raloxifene in Rat Plasma Mahmood, Syed Sengupta, Pinaki Mandal, Uttam K. Chatterjee, Bappaditya Taher, Muhammad RS Pharmacy and materia medica SUMMARY. A simple and sensitive HPLC method has been developed and validated for the quantification of raloxifene in rat plasma. Liquid- liquid extraction procedure was employed for extracting raloxifene from rat plasma sample. Separation of raloxifene was achieved through a RP- C18 column with a mobile phase consisted of phosphate buffer and acetonitrile (66:34 %, v/v). The method was validated for specificity, selectivity, sensitivity, linearity, accuracy, precision, recovery and stability parameters. A linear response (R2 value 0.9991) was found over the calibration range of 50 to 500 ng/mL. The accuracy for intra and inter day run varied between 86.73 to 102.30 % and 91.11 to 95.56%, respectively. The corresponding precision (% CV) were within 0.82 to 9.43% and 6.23 to 8.33%. The method was also found to be specific and stable. The applicability of the method was established through a single dose oral pharmacokinetic study of raloxifene in rat. College of Pharmacists of Buenos Aires Province, Argentina 2017-07 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/19791/1/ftech-2017-mahmood-Development%2C%20Validation%20and%20Pharmacokinetic%20Application1.pdf Mahmood, Syed and Sengupta, Pinaki and Mandal, Uttam K. and Chatterjee, Bappaditya and Taher, Muhammad (2017) Development, Validation and Pharmacokinetic Application of a Simple and Robust RP-HPLC Method for Quantitation of Raloxifene in Rat Plasma. Latin American Journal of Pharmacy, 36 (9). pp. 1901-1908. ISSN 2362-3853 http://www.latamjpharm.org/resumenes/36/9/LAJOP_36_9_1_35.pdf http://www.latamjpharm.org/previous_issue.php?vol=36&num=9 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
Universiti Malaysia Pahang |
| building |
UMP Institutional Repository |
| collection |
Online Access |
| language |
English |
| topic |
RS Pharmacy and materia medica |
| spellingShingle |
RS Pharmacy and materia medica Mahmood, Syed Sengupta, Pinaki Mandal, Uttam K. Chatterjee, Bappaditya Taher, Muhammad Development, Validation and Pharmacokinetic Application of a Simple and Robust RP-HPLC Method for Quantitation of Raloxifene in Rat Plasma |
| description |
SUMMARY. A simple and sensitive HPLC method has been developed and validated for the quantification
of raloxifene in rat plasma. Liquid- liquid extraction procedure was employed for extracting raloxifene
from rat plasma sample. Separation of raloxifene was achieved through a RP- C18 column with a mobile
phase consisted of phosphate buffer and acetonitrile (66:34 %, v/v). The method was validated for specificity,
selectivity, sensitivity, linearity, accuracy, precision, recovery and stability parameters. A linear response
(R2 value 0.9991) was found over the calibration range of 50 to 500 ng/mL. The accuracy for intra
and inter day run varied between 86.73 to 102.30 % and 91.11 to 95.56%, respectively. The corresponding
precision (% CV) were within 0.82 to 9.43% and 6.23 to 8.33%. The method was also found to be specific
and stable. The applicability of the method was established through a single dose oral pharmacokinetic
study of raloxifene in rat. |
| format |
Article |
| author |
Mahmood, Syed Sengupta, Pinaki Mandal, Uttam K. Chatterjee, Bappaditya Taher, Muhammad |
| author_facet |
Mahmood, Syed Sengupta, Pinaki Mandal, Uttam K. Chatterjee, Bappaditya Taher, Muhammad |
| author_sort |
Mahmood, Syed |
| title |
Development, Validation and Pharmacokinetic Application
of a Simple and Robust RP-HPLC Method for Quantitation
of Raloxifene in Rat Plasma |
| title_short |
Development, Validation and Pharmacokinetic Application
of a Simple and Robust RP-HPLC Method for Quantitation
of Raloxifene in Rat Plasma |
| title_full |
Development, Validation and Pharmacokinetic Application
of a Simple and Robust RP-HPLC Method for Quantitation
of Raloxifene in Rat Plasma |
| title_fullStr |
Development, Validation and Pharmacokinetic Application
of a Simple and Robust RP-HPLC Method for Quantitation
of Raloxifene in Rat Plasma |
| title_full_unstemmed |
Development, Validation and Pharmacokinetic Application
of a Simple and Robust RP-HPLC Method for Quantitation
of Raloxifene in Rat Plasma |
| title_sort |
development, validation and pharmacokinetic application
of a simple and robust rp-hplc method for quantitation
of raloxifene in rat plasma |
| publisher |
College of Pharmacists of Buenos Aires Province, Argentina |
| publishDate |
2017 |
| url |
http://umpir.ump.edu.my/id/eprint/19791/ http://umpir.ump.edu.my/id/eprint/19791/ http://umpir.ump.edu.my/id/eprint/19791/ http://umpir.ump.edu.my/id/eprint/19791/1/ftech-2017-mahmood-Development%2C%20Validation%20and%20Pharmacokinetic%20Application1.pdf |
| first_indexed |
2023-09-18T22:28:23Z |
| last_indexed |
2023-09-18T22:28:23Z |
| _version_ |
1777416120399036416 |