A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation

This article presents the comparison of reusability and leakage between entrapped and covalently bonded laccase and their performances towards the selective oxidation of glycerol. The reusability of immobilized laccase enzyme was studied by reacting a batch of immobilized laccase with ABTS for 15 cy...

Full description

Bibliographic Details
Main Authors:	Hong, Chi Shein, Lau Cindy, Chin Yee, Leong, Chun Yi, Chua, Gek Kee, Chin, S. Y.
Format:	Article
Language:	English
Published:	Taylor & Francis 2018
Subjects:	QD Chemistry TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/19265/ http://umpir.ump.edu.my/id/eprint/19265/ http://umpir.ump.edu.my/id/eprint/19265/ http://umpir.ump.edu.my/id/eprint/19265/7/A%20comparison%20of%20entrapped%20and%20covalently%20bonded1.pdf

id	ump-19265
recordtype	eprints
spelling	ump-192652019-01-18T03:08:16Z http://umpir.ump.edu.my/id/eprint/19265/ A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation Hong, Chi Shein Lau Cindy, Chin Yee Leong, Chun Yi Chua, Gek Kee Chin, S. Y. QD Chemistry TP Chemical technology This article presents the comparison of reusability and leakage between entrapped and covalently bonded laccase and their performances towards the selective oxidation of glycerol. The reusability of immobilized laccase enzyme was studied by reacting a batch of immobilized laccase with ABTS for 15 cycles. The investigation of the leakage of immobilized laccase was carried out by storing the immobilized laccase in acetate buffer solution for 32 days. The data show that the retained enzyme activities of entrapped and covalently bonded enzyme after being reused for eight cycles were well above 60% and the leakages after storing for a month in the acetate buffer at 4C were well below 15%. The entrapped laccase coupled with TEMPO was found to perform better and gave a two-fold higher yield of glyceraldehyde and glyceric acid in the selective oxidation of glycerol compared to covalently bonded laccase. Hence, physical entrapment of laccase would be a suitable immobilization method in the laccase-mediated selective oxidation of glycerol. Taylor & Francis 2018 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/19265/7/A%20comparison%20of%20entrapped%20and%20covalently%20bonded1.pdf Hong, Chi Shein and Lau Cindy, Chin Yee and Leong, Chun Yi and Chua, Gek Kee and Chin, S. Y. (2018) A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation. Biocatalysis and Biotransformation, 36 (5). pp. 352-361. ISSN 1024-2422 (Print); 1029-2446 (Online) https://doi.org/10.1080/10242422.2017.1384467 10.1080/10242422.2017.1384467
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QD Chemistry TP Chemical technology
spellingShingle	QD Chemistry TP Chemical technology Hong, Chi Shein Lau Cindy, Chin Yee Leong, Chun Yi Chua, Gek Kee Chin, S. Y. A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation
description	This article presents the comparison of reusability and leakage between entrapped and covalently bonded laccase and their performances towards the selective oxidation of glycerol. The reusability of immobilized laccase enzyme was studied by reacting a batch of immobilized laccase with ABTS for 15 cycles. The investigation of the leakage of immobilized laccase was carried out by storing the immobilized laccase in acetate buffer solution for 32 days. The data show that the retained enzyme activities of entrapped and covalently bonded enzyme after being reused for eight cycles were well above 60% and the leakages after storing for a month in the acetate buffer at 4C were well below 15%. The entrapped laccase coupled with TEMPO was found to perform better and gave a two-fold higher yield of glyceraldehyde and glyceric acid in the selective oxidation of glycerol compared to covalently bonded laccase. Hence, physical entrapment of laccase would be a suitable immobilization method in the laccase-mediated selective oxidation of glycerol.
format	Article
author	Hong, Chi Shein Lau Cindy, Chin Yee Leong, Chun Yi Chua, Gek Kee Chin, S. Y.
author_facet	Hong, Chi Shein Lau Cindy, Chin Yee Leong, Chun Yi Chua, Gek Kee Chin, S. Y.
author_sort	Hong, Chi Shein
title	A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation
title_short	A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation
title_full	A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation
title_fullStr	A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation
title_full_unstemmed	A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation
title_sort	comparison of entrapped and covalently bonded laccase: study of its leakage, reusability, and the catalytic efficiency in tempo-mediated glycerol oxidation
publisher	Taylor & Francis
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/19265/ http://umpir.ump.edu.my/id/eprint/19265/ http://umpir.ump.edu.my/id/eprint/19265/ http://umpir.ump.edu.my/id/eprint/19265/7/A%20comparison%20of%20entrapped%20and%20covalently%20bonded1.pdf
first_indexed	2023-09-18T22:27:37Z
last_indexed	2023-09-18T22:27:37Z
_version_	1777416072180269056

A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation

Similar Items