Lipopolysaccharide Induces The Production Of Diagnostic Monoclonal Antibody By Hybridoma Cells Against Congenital Adrenal Hyperplasia

The purpose of this research is to screen and identify the potential inducers in maximizing the production of monoclonal antibody by hybridoma 192 cell line for Congenital Adrenal Hyperplasia diagnostic. There are nine inducers used in this research, namely lysozyme, aldolase, sodium butyrate, sodiu...

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Main Authors:	Chua, Gek Kee, Nur Anis, Mohamad Ariff
Format:	Article
Language:	English
Published:	School of Engineering, Taylor's University 2017
Subjects:	Q Science (General) T Technology (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/19264/ http://umpir.ump.edu.my/id/eprint/19264/ http://umpir.ump.edu.my/id/eprint/19264/1/LPS%20paper%20in%20JESTEC.pdf

id	ump-19264
recordtype	eprints
spelling	ump-192642018-05-02T04:02:09Z http://umpir.ump.edu.my/id/eprint/19264/ Lipopolysaccharide Induces The Production Of Diagnostic Monoclonal Antibody By Hybridoma Cells Against Congenital Adrenal Hyperplasia Chua, Gek Kee Nur Anis, Mohamad Ariff Q Science (General) T Technology (General) The purpose of this research is to screen and identify the potential inducers in maximizing the production of monoclonal antibody by hybridoma 192 cell line for Congenital Adrenal Hyperplasia diagnostic. There are nine inducers used in this research, namely lysozyme, aldolase, sodium butyrate, sodium phosphate, potassium phosphate, dimethyl sulfoxide, lipopolysaccharide, essential amino acids, and nonessential amino acids. Hybridoma 192 cell was cultured in 5% CO2 incubator at 37°C and ˃80% humidity in the medium with different concentrations of inducer agents. The inducers were added at the beginning of the culture and the samples were taken after 72 h of culture. The performance of these inducer agents was assessed based on the maximum monoclonal antibody titer achieved using Enzyme-linked Immunosorbent Assay. Lipopolysaccharide was found to increase the maximum monoclonal antibody titer when supplemented at 8 to 12 µg/mL. After optimization using one-factor central composite design at this range, the optimum point was determined to be 8 µg/mL. Verification experiments show that lipopolysaccharide enhanced the average specific monoclonal antibody production rate by 56% relative to control. In conclusion, lipopolysaccharide at 8 µg/mL is able to increase the monoclonal antibody specific production of hybridoma 192 cell line. School of Engineering, Taylor's University 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/19264/1/LPS%20paper%20in%20JESTEC.pdf Chua, Gek Kee and Nur Anis, Mohamad Ariff (2017) Lipopolysaccharide Induces The Production Of Diagnostic Monoclonal Antibody By Hybridoma Cells Against Congenital Adrenal Hyperplasia. Journal of Engineering Science and Technology, 12 (11). pp. 2939-2951. ISSN 1823-4690 http://jestec.taylors.edu.my/V12Issue11.htm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General) T Technology (General)
spellingShingle	Q Science (General) T Technology (General) Chua, Gek Kee Nur Anis, Mohamad Ariff Lipopolysaccharide Induces The Production Of Diagnostic Monoclonal Antibody By Hybridoma Cells Against Congenital Adrenal Hyperplasia
description	The purpose of this research is to screen and identify the potential inducers in maximizing the production of monoclonal antibody by hybridoma 192 cell line for Congenital Adrenal Hyperplasia diagnostic. There are nine inducers used in this research, namely lysozyme, aldolase, sodium butyrate, sodium phosphate, potassium phosphate, dimethyl sulfoxide, lipopolysaccharide, essential amino acids, and nonessential amino acids. Hybridoma 192 cell was cultured in 5% CO2 incubator at 37°C and ˃80% humidity in the medium with different concentrations of inducer agents. The inducers were added at the beginning of the culture and the samples were taken after 72 h of culture. The performance of these inducer agents was assessed based on the maximum monoclonal antibody titer achieved using Enzyme-linked Immunosorbent Assay. Lipopolysaccharide was found to increase the maximum monoclonal antibody titer when supplemented at 8 to 12 µg/mL. After optimization using one-factor central composite design at this range, the optimum point was determined to be 8 µg/mL. Verification experiments show that lipopolysaccharide enhanced the average specific monoclonal antibody production rate by 56% relative to control. In conclusion, lipopolysaccharide at 8 µg/mL is able to increase the monoclonal antibody specific production of hybridoma 192 cell line.
format	Article
author	Chua, Gek Kee Nur Anis, Mohamad Ariff
author_facet	Chua, Gek Kee Nur Anis, Mohamad Ariff
author_sort	Chua, Gek Kee
title	Lipopolysaccharide Induces The Production Of Diagnostic Monoclonal Antibody By Hybridoma Cells Against Congenital Adrenal Hyperplasia
title_short	Lipopolysaccharide Induces The Production Of Diagnostic Monoclonal Antibody By Hybridoma Cells Against Congenital Adrenal Hyperplasia
title_full	Lipopolysaccharide Induces The Production Of Diagnostic Monoclonal Antibody By Hybridoma Cells Against Congenital Adrenal Hyperplasia
title_fullStr	Lipopolysaccharide Induces The Production Of Diagnostic Monoclonal Antibody By Hybridoma Cells Against Congenital Adrenal Hyperplasia
title_full_unstemmed	Lipopolysaccharide Induces The Production Of Diagnostic Monoclonal Antibody By Hybridoma Cells Against Congenital Adrenal Hyperplasia
title_sort	lipopolysaccharide induces the production of diagnostic monoclonal antibody by hybridoma cells against congenital adrenal hyperplasia
publisher	School of Engineering, Taylor's University
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/19264/ http://umpir.ump.edu.my/id/eprint/19264/ http://umpir.ump.edu.my/id/eprint/19264/1/LPS%20paper%20in%20JESTEC.pdf
first_indexed	2023-09-18T22:27:37Z
last_indexed	2023-09-18T22:27:37Z
_version_	1777416072035565568

Lipopolysaccharide Induces The Production Of Diagnostic Monoclonal Antibody By Hybridoma Cells Against Congenital Adrenal Hyperplasia

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