Biodegradation of Pyrene by Candida sp. S1 under High Salinity Conditions
Polycyclic aromatic hydrocarbon is a toxic recalcitrant environmental pollutant and its removal from the environment is very essential. In this study, a novel S1 strain isolated from the tropical rain forest was identified as Candida species based on 18S rRNA. The pyrene biodegradation was performed...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer
2017
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/18099/ http://umpir.ump.edu.my/id/eprint/18099/ http://umpir.ump.edu.my/id/eprint/18099/ http://umpir.ump.edu.my/id/eprint/18099/1/ftech-2017-risky-Biodegradation%20of%20pyrene%20by%20Candida1.pdf |
| Summary: | Polycyclic aromatic hydrocarbon is a toxic recalcitrant environmental pollutant and its removal from the environment is very essential. In this study, a novel S1 strain isolated from the tropical rain forest was identified as Candida species based on 18S rRNA. The pyrene biodegradation was performed by Candida sp. S1. Pyrene was 35% degraded in 15 days. The percentage of pyrene biodegradation increased up to 75% with 24 g L-1 of sodium chloride and decreased along with increasing salinity. Under the acidic condition, the biodegradation was increased up to 60% at pH 5. It was also found that the increasing glucose concentration of more than 10 g L-1 had no significant effect on pyrene biodegradation, while agitation proved to have greater influence. There was a positive relationship between biomass growth and
biodegradation rate of pyrene. One pyrene metabolite was
identified from the extract solution and analyzed by a thinlayer chromatography, UV–visible absorption and gas
chromatography–mass spectrometry. The metabolite found
in the pyrene degradation was benzoic acid. |
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