Characterization of Keratin Microparticles from Feather Biomass with Potent Antioxidant and Anticancer Activities

In the present study chicken feathers were hydrolyzed by chemical treatment in alkaline conditions. The pH value of feather hydrolyzed solution was amended accordingly the iso-electric precipitation. Two types of keratin microparticles KM1, KM2 were synthesized under acidic conditions at 3.5 and 5.5...

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Main Authors:	Sharma, Swati, Gupta, Arun, Syed Mohd Saufi, Tuan Chik, Chua, Gek Kee, Mistry, Bhupendra M., Kim, Doo H., Sharma, Gaurav
Format:	Article
Language:	English
Published:	Elsevier Ltd 2017
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/18041/ http://umpir.ump.edu.my/id/eprint/18041/ http://umpir.ump.edu.my/id/eprint/18041/ http://umpir.ump.edu.my/id/eprint/18041/2/Characterization%20of%20keratin%20microparticles%20from%20feather%20biomass%20with%20potent%20antioxidant%20and%20anticancer%20activities%201.pdf

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spelling	ump-180412019-09-10T03:08:13Z http://umpir.ump.edu.my/id/eprint/18041/ Characterization of Keratin Microparticles from Feather Biomass with Potent Antioxidant and Anticancer Activities Sharma, Swati Gupta, Arun Syed Mohd Saufi, Tuan Chik Chua, Gek Kee Mistry, Bhupendra M. Kim, Doo H. Sharma, Gaurav TP Chemical technology In the present study chicken feathers were hydrolyzed by chemical treatment in alkaline conditions. The pH value of feather hydrolyzed solution was amended accordingly the iso-electric precipitation. Two types of keratin microparticles KM1, KM2 were synthesized under acidic conditions at 3.5 and 5.5 pH respectively. The synthesized keratin microparticles possessed uniform and round surface by scanning electron microscopy (SEM). The thermal degradation of microparticles were examined by thermogravimetry (TGA). Fourier transform infrared spectroscopy (FTIR) revealed that the extracted keratin retained the most of protein backbone. The microparticles were screened for their in vitro anticancer activities by SRB bioassay towards HeLa, SK-OV-3 and A549 cancer cell lines. Futhermore, their cytotoxicity towards healthy cell lines was analyzed having Malin Darby canine kidney (MDCK) cell lines along with in vitro antioxidant activity using DPPH and ABTS methods KM1 and KM2 showed 200.31 ± 1.01 and 139.73 ± 0.94, 214.16 ± 0.29 and 153.92 ± 0.61, 328.92 ± 3.46 and 200.33 ± 2.48 μg/mL of IC50 levels against HeLa, SK-OV-3, and A549 cell lines, respectively. Moreover, KM1 and KM2 demonstrated significant antioxidant potency with IC50 levels 13.15 and 9.02 μg/mL as well as 8.96 and 5.60 μg/mL in DPPH and ABTS radical scavenging bioassay, respectively. Elsevier Ltd 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/18041/2/Characterization%20of%20keratin%20microparticles%20from%20feather%20biomass%20with%20potent%20antioxidant%20and%20anticancer%20activities%201.pdf Sharma, Swati and Gupta, Arun and Syed Mohd Saufi, Tuan Chik and Chua, Gek Kee and Mistry, Bhupendra M. and Kim, Doo H. and Sharma, Gaurav (2017) Characterization of Keratin Microparticles from Feather Biomass with Potent Antioxidant and Anticancer Activities. International Journal of Biological Macromolecules, 104. pp. 189-196. ISSN 0141-8130 https://doi.org/10.1016/j.ijbiomac.2017.06.015 DOI: 10.1016/j.ijbiomac.2017.06.015
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Sharma, Swati Gupta, Arun Syed Mohd Saufi, Tuan Chik Chua, Gek Kee Mistry, Bhupendra M. Kim, Doo H. Sharma, Gaurav Characterization of Keratin Microparticles from Feather Biomass with Potent Antioxidant and Anticancer Activities
description	In the present study chicken feathers were hydrolyzed by chemical treatment in alkaline conditions. The pH value of feather hydrolyzed solution was amended accordingly the iso-electric precipitation. Two types of keratin microparticles KM1, KM2 were synthesized under acidic conditions at 3.5 and 5.5 pH respectively. The synthesized keratin microparticles possessed uniform and round surface by scanning electron microscopy (SEM). The thermal degradation of microparticles were examined by thermogravimetry (TGA). Fourier transform infrared spectroscopy (FTIR) revealed that the extracted keratin retained the most of protein backbone. The microparticles were screened for their in vitro anticancer activities by SRB bioassay towards HeLa, SK-OV-3 and A549 cancer cell lines. Futhermore, their cytotoxicity towards healthy cell lines was analyzed having Malin Darby canine kidney (MDCK) cell lines along with in vitro antioxidant activity using DPPH and ABTS methods KM1 and KM2 showed 200.31 ± 1.01 and 139.73 ± 0.94, 214.16 ± 0.29 and 153.92 ± 0.61, 328.92 ± 3.46 and 200.33 ± 2.48 μg/mL of IC50 levels against HeLa, SK-OV-3, and A549 cell lines, respectively. Moreover, KM1 and KM2 demonstrated significant antioxidant potency with IC50 levels 13.15 and 9.02 μg/mL as well as 8.96 and 5.60 μg/mL in DPPH and ABTS radical scavenging bioassay, respectively.
format	Article
author	Sharma, Swati Gupta, Arun Syed Mohd Saufi, Tuan Chik Chua, Gek Kee Mistry, Bhupendra M. Kim, Doo H. Sharma, Gaurav
author_facet	Sharma, Swati Gupta, Arun Syed Mohd Saufi, Tuan Chik Chua, Gek Kee Mistry, Bhupendra M. Kim, Doo H. Sharma, Gaurav
author_sort	Sharma, Swati
title	Characterization of Keratin Microparticles from Feather Biomass with Potent Antioxidant and Anticancer Activities
title_short	Characterization of Keratin Microparticles from Feather Biomass with Potent Antioxidant and Anticancer Activities
title_full	Characterization of Keratin Microparticles from Feather Biomass with Potent Antioxidant and Anticancer Activities
title_fullStr	Characterization of Keratin Microparticles from Feather Biomass with Potent Antioxidant and Anticancer Activities
title_full_unstemmed	Characterization of Keratin Microparticles from Feather Biomass with Potent Antioxidant and Anticancer Activities
title_sort	characterization of keratin microparticles from feather biomass with potent antioxidant and anticancer activities
publisher	Elsevier Ltd
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/18041/ http://umpir.ump.edu.my/id/eprint/18041/ http://umpir.ump.edu.my/id/eprint/18041/ http://umpir.ump.edu.my/id/eprint/18041/2/Characterization%20of%20keratin%20microparticles%20from%20feather%20biomass%20with%20potent%20antioxidant%20and%20anticancer%20activities%201.pdf
first_indexed	2023-09-18T22:25:18Z
last_indexed	2023-09-18T22:25:18Z
_version_	1777415926339076096

Characterization of Keratin Microparticles from Feather Biomass with Potent Antioxidant and Anticancer Activities

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