Statistical Investigation of Extraction Parameters of Keratin from Chicken Feather using Design-Expert

Statistical Investigation of Extraction Parameters of Keratin from Chicken Feather using Design-Expert

Uncontrolled disposal of feathers from the poultry industry and slaughterhouses is environmentally undesirable. The feathers are composed of approximately 90% of keratin which is an important ingredient of cosmetics, shampoos and hair treatment creams. This study aimed to determine the optimum condi...

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Main Authors: Nuruldiyanah, Kamarudin, Sharma, Swati, Chua, Gek Kee, Syed Mohd Saufi, Tuan Chik, Ritu, Gupta
Format: Article
Language:English
Published: Springer Berlin Heidelberg 2017
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/17913/
http://umpir.ump.edu.my/id/eprint/17913/
http://umpir.ump.edu.my/id/eprint/17913/
http://umpir.ump.edu.my/id/eprint/17913/1/Statistical%20investigation%20of%20extraction%20parameters%20of%20keratin-fkksa-2017.pdf
id ump-17913
recordtype eprints
spelling ump-179132019-09-10T03:09:10Z http://umpir.ump.edu.my/id/eprint/17913/ Statistical Investigation of Extraction Parameters of Keratin from Chicken Feather using Design-Expert Nuruldiyanah, Kamarudin Sharma, Swati Chua, Gek Kee Syed Mohd Saufi, Tuan Chik Ritu, Gupta TP Chemical technology Uncontrolled disposal of feathers from the poultry industry and slaughterhouses is environmentally undesirable. The feathers are composed of approximately 90% of keratin which is an important ingredient of cosmetics, shampoos and hair treatment creams. This study aimed to determine the optimum conditions for the extraction of keratin from chicken feathers. The extraction of keratin using various reducing agents was studied using statistical experimental design. In the extraction process, pH, temperature, ratio of reducing agents, mass of chicken feathers and incubation time were analyzed. The keratin in the total extracted protein was purified by size exclusion chromatography, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and further characterized using amino acids profile analysis. The surface morphology and chemical composition were studied by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) analysis. Sodium sulfide (Na2S) yielded 84.5% of keratin as compared to sodium hydroxide (43.8), urea mixture (50.6), mixture of sodium dodecyl sulfate (SDS) and sodium bisulfite (18.3) and a mixture of Na2S and sodium hydroxide (41.5%) under optimized conditions. The optimum yield of keratin was achieved at 80.9 °C in 9.5 h with 0.05 M sodium sulfide using response surface methodology (RSM). Among the five parameters screened, pH was found not to be significant because the p value was greater than 0.05. Springer Berlin Heidelberg 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/17913/1/Statistical%20investigation%20of%20extraction%20parameters%20of%20keratin-fkksa-2017.pdf Nuruldiyanah, Kamarudin and Sharma, Swati and Chua, Gek Kee and Syed Mohd Saufi, Tuan Chik and Ritu, Gupta (2017) Statistical Investigation of Extraction Parameters of Keratin from Chicken Feather using Design-Expert. 3 Biotech, 7 (127). pp. 1-9. ISSN 2190-572X (print); 2190-5738 (online) https://doi.org/10.1007/s13205-017-0767-9 doi: 10.1007/s13205-017-0767-9
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Nuruldiyanah, Kamarudin
Sharma, Swati
Chua, Gek Kee
Syed Mohd Saufi, Tuan Chik
Ritu, Gupta
Statistical Investigation of Extraction Parameters of Keratin from Chicken Feather using Design-Expert
description Uncontrolled disposal of feathers from the poultry industry and slaughterhouses is environmentally undesirable. The feathers are composed of approximately 90% of keratin which is an important ingredient of cosmetics, shampoos and hair treatment creams. This study aimed to determine the optimum conditions for the extraction of keratin from chicken feathers. The extraction of keratin using various reducing agents was studied using statistical experimental design. In the extraction process, pH, temperature, ratio of reducing agents, mass of chicken feathers and incubation time were analyzed. The keratin in the total extracted protein was purified by size exclusion chromatography, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and further characterized using amino acids profile analysis. The surface morphology and chemical composition were studied by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) analysis. Sodium sulfide (Na2S) yielded 84.5% of keratin as compared to sodium hydroxide (43.8), urea mixture (50.6), mixture of sodium dodecyl sulfate (SDS) and sodium bisulfite (18.3) and a mixture of Na2S and sodium hydroxide (41.5%) under optimized conditions. The optimum yield of keratin was achieved at 80.9 °C in 9.5 h with 0.05 M sodium sulfide using response surface methodology (RSM). Among the five parameters screened, pH was found not to be significant because the p value was greater than 0.05.
format Article
author Nuruldiyanah, Kamarudin
Sharma, Swati
Chua, Gek Kee
Syed Mohd Saufi, Tuan Chik
Ritu, Gupta
author_facet Nuruldiyanah, Kamarudin
Sharma, Swati
Chua, Gek Kee
Syed Mohd Saufi, Tuan Chik
Ritu, Gupta
author_sort Nuruldiyanah, Kamarudin
title Statistical Investigation of Extraction Parameters of Keratin from Chicken Feather using Design-Expert
title_short Statistical Investigation of Extraction Parameters of Keratin from Chicken Feather using Design-Expert
title_full Statistical Investigation of Extraction Parameters of Keratin from Chicken Feather using Design-Expert
title_fullStr Statistical Investigation of Extraction Parameters of Keratin from Chicken Feather using Design-Expert
title_full_unstemmed Statistical Investigation of Extraction Parameters of Keratin from Chicken Feather using Design-Expert
title_sort statistical investigation of extraction parameters of keratin from chicken feather using design-expert
publisher Springer Berlin Heidelberg
publishDate 2017
url http://umpir.ump.edu.my/id/eprint/17913/
http://umpir.ump.edu.my/id/eprint/17913/
http://umpir.ump.edu.my/id/eprint/17913/
http://umpir.ump.edu.my/id/eprint/17913/1/Statistical%20investigation%20of%20extraction%20parameters%20of%20keratin-fkksa-2017.pdf
first_indexed 2023-09-18T22:25:02Z
last_indexed 2023-09-18T22:25:02Z
_version_ 1777415909945638912

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