Bioremediation of Disposed Engine Oil for Lipase Production

Engine oil is one of the several refined products of crude oil. It is composed of long chain saturated hydrocarbons (base oil) additives. Generally, engine oil can enter into the environment through leak of oil tankers, cleaning of tanks by merchants, warship carrying engine oil, and operations by m...

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Main Authors:	Mahmood, Mahmood H., Zhi, Yang, Thanoon, Raid D., Makky, Essam A., Mohd Hasbi, Ab. Rahim
Format:	Conference or Workshop Item
Language:	English
Published:	Universiti Malaysia Pahang 2017
Subjects:	TD Environmental technology. Sanitary engineering
Online Access:	http://umpir.ump.edu.my/id/eprint/17621/ http://umpir.ump.edu.my/id/eprint/17621/ http://umpir.ump.edu.my/id/eprint/17621/1/18.%20Bioremediation%20of%20disposed%20engine%20oil%20for%20lipase%20production.pdf

id	ump-17621
recordtype	eprints
spelling	ump-176212020-02-28T01:26:51Z http://umpir.ump.edu.my/id/eprint/17621/ Bioremediation of Disposed Engine Oil for Lipase Production Mahmood, Mahmood H. Zhi, Yang Thanoon, Raid D. Makky, Essam A. Mohd Hasbi, Ab. Rahim TD Environmental technology. Sanitary engineering Engine oil is one of the several refined products of crude oil. It is composed of long chain saturated hydrocarbons (base oil) additives. Generally, engine oil can enter into the environment through leak of oil tankers, cleaning of tanks by merchants, warship carrying engine oil, and operations by motor mechanics which is a common industrial waste that is harmful to environment and human health. This research aims to produce lipase enzyme using microbial degradation of disposed engine oil. In this study hydrocarbon-degrading bacterium, GS-3 was successfully isolated from oil contaminated area. GC-MS analysis revealed that this isolate was able to produce organic acid, methyl-3,4,5-trimethoxy-2,6-dinitrobenzoate from disposed engine oil. Besides, GS-3 isolate produced highest lipase activity, achieving 0.097±0.007 Uml-1min-1 during first 24 hrs when disposed engine oil (DEO) was used as carbon source. Data revealed new and broad band which is related to O-H stretching formed at 3421 cm-1, though new band occurred at 3424 cm-1 and 1645 cm-1 after bioremediation. Subsequent lipase optimization parameters revealed that this bacterial isolate could produce highest lipase activity when the 4% (v/v) DEO was used as carbon source. The best nitrogen source was urea. Addition of surfactant Tween 80 could also enhanced lipase production. Optimal pH value and temperature was 7.0 and 30°C respectively. Universiti Malaysia Pahang 2017 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/17621/1/18.%20Bioremediation%20of%20disposed%20engine%20oil%20for%20lipase%20production.pdf Mahmood, Mahmood H. and Zhi, Yang and Thanoon, Raid D. and Makky, Essam A. and Mohd Hasbi, Ab. Rahim (2017) Bioremediation of Disposed Engine Oil for Lipase Production. In: Proceedings of the FGIC 1st Conference on Governance & Integrity, 3-4 April 2017 , Yayasan Pahang, Kuantan, Malaysia. pp. 337-354.. ISBN 978-967-2054-37-5 http://fgic.ump.edu.my/images/docman/1st-FGIC-Proceedings.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TD Environmental technology. Sanitary engineering
spellingShingle	TD Environmental technology. Sanitary engineering Mahmood, Mahmood H. Zhi, Yang Thanoon, Raid D. Makky, Essam A. Mohd Hasbi, Ab. Rahim Bioremediation of Disposed Engine Oil for Lipase Production
description	Engine oil is one of the several refined products of crude oil. It is composed of long chain saturated hydrocarbons (base oil) additives. Generally, engine oil can enter into the environment through leak of oil tankers, cleaning of tanks by merchants, warship carrying engine oil, and operations by motor mechanics which is a common industrial waste that is harmful to environment and human health. This research aims to produce lipase enzyme using microbial degradation of disposed engine oil. In this study hydrocarbon-degrading bacterium, GS-3 was successfully isolated from oil contaminated area. GC-MS analysis revealed that this isolate was able to produce organic acid, methyl-3,4,5-trimethoxy-2,6-dinitrobenzoate from disposed engine oil. Besides, GS-3 isolate produced highest lipase activity, achieving 0.097±0.007 Uml-1min-1 during first 24 hrs when disposed engine oil (DEO) was used as carbon source. Data revealed new and broad band which is related to O-H stretching formed at 3421 cm-1, though new band occurred at 3424 cm-1 and 1645 cm-1 after bioremediation. Subsequent lipase optimization parameters revealed that this bacterial isolate could produce highest lipase activity when the 4% (v/v) DEO was used as carbon source. The best nitrogen source was urea. Addition of surfactant Tween 80 could also enhanced lipase production. Optimal pH value and temperature was 7.0 and 30°C respectively.
format	Conference or Workshop Item
author	Mahmood, Mahmood H. Zhi, Yang Thanoon, Raid D. Makky, Essam A. Mohd Hasbi, Ab. Rahim
author_facet	Mahmood, Mahmood H. Zhi, Yang Thanoon, Raid D. Makky, Essam A. Mohd Hasbi, Ab. Rahim
author_sort	Mahmood, Mahmood H.
title	Bioremediation of Disposed Engine Oil for Lipase Production
title_short	Bioremediation of Disposed Engine Oil for Lipase Production
title_full	Bioremediation of Disposed Engine Oil for Lipase Production
title_fullStr	Bioremediation of Disposed Engine Oil for Lipase Production
title_full_unstemmed	Bioremediation of Disposed Engine Oil for Lipase Production
title_sort	bioremediation of disposed engine oil for lipase production
publisher	Universiti Malaysia Pahang
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/17621/ http://umpir.ump.edu.my/id/eprint/17621/ http://umpir.ump.edu.my/id/eprint/17621/1/18.%20Bioremediation%20of%20disposed%20engine%20oil%20for%20lipase%20production.pdf
first_indexed	2023-09-18T22:24:27Z
last_indexed	2023-09-18T22:24:27Z
_version_	1777415872709656576

Bioremediation of Disposed Engine Oil for Lipase Production

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