Assessment of Sulfide Concentration Effects in a Micro-Oxygenated Condition on the Growth and Removal by Pseudomonas putida (ATCC 49128)

Micro-oxygenation has recently being gaining popularity as a successful biological sulfide oxidation technique from inflicted wastewater. In this study the application of orbital shaker as an alternative digester for sulfi de bioxidation was experimented using Pseudomonas putida (ATCC 49128),...

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Main Authors:	Ahmad, Mani Malam, Abdul Aziz, Mohd Azoddein, Mior Ahmad Khushairi, Mohd Zahari, Mazrul Nizam, Abu Seman, Mohammad Saedi, Jami, Faten Ahada, Mohd Azli
Format:	Article
Language:	English
Published:	Penerbit Akademia Baru 2016
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/17566/ http://umpir.ump.edu.my/id/eprint/17566/ http://umpir.ump.edu.my/id/eprint/17566/1/fkksa-2016-mazrul-Assessment%20of%20sulfide%20concentration%20effects.pdf

id	ump-17566
recordtype	eprints
spelling	ump-175662018-01-22T03:21:46Z http://umpir.ump.edu.my/id/eprint/17566/ Assessment of Sulfide Concentration Effects in a Micro-Oxygenated Condition on the Growth and Removal by Pseudomonas putida (ATCC 49128) Ahmad, Mani Malam Abdul Aziz, Mohd Azoddein Mior Ahmad Khushairi, Mohd Zahari Mazrul Nizam, Abu Seman Mohammad Saedi, Jami Faten Ahada, Mohd Azli TP Chemical technology Micro-oxygenation has recently being gaining popularity as a successful biological sulfide oxidation technique from inflicted wastewater. In this study the application of orbital shaker as an alternative digester for sulfi de bioxidation was experimented using Pseudomonas putida (ATCC 49128), under an oxygen tension or micro-oxygenated condition. Growth and sulfide reduction efficiency was measured spectrophotometrically under optimum physical conditions of pH, temperature, acclimatization time and agitation. Sulfide reduction was overwhelmingly recorded at three different sulfide loading rates of 200mM S2- L-1 d-1, 300mM S2 L-d-1 and 500mM S2-L-d-1 with corresponding appreciable cell growth measured at OD600nm. The obtained results indicated that it was possible to realized sulfide removal efficiency of 96% to 100% within 24hrs, as well 45% to 70% within the first 6hrs of inoculation, with an overwhelming removal of 100% after 18hrs in 200mM and 300mM. On the other hand, the corresponding growth was 3.00 OD600nm, 2.824 OD600nm and 2.456 OD600nm in 500pmm, 200ppm and 300ppm concentration respectively. Based on this finding, it was clear that this inoculum can be employed to treat sulfide contaminated wastewater even at higher range under micro-oxygena ted environmental condition. Penerbit Akademia Baru 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/17566/1/fkksa-2016-mazrul-Assessment%20of%20sulfide%20concentration%20effects.pdf Ahmad, Mani Malam and Abdul Aziz, Mohd Azoddein and Mior Ahmad Khushairi, Mohd Zahari and Mazrul Nizam, Abu Seman and Mohammad Saedi, Jami and Faten Ahada, Mohd Azli (2016) Assessment of Sulfide Concentration Effects in a Micro-Oxygenated Condition on the Growth and Removal by Pseudomonas putida (ATCC 49128). Journal of Advanced Research in Materials Science, 27 (1). pp. 1-10. ISSN 2289-7992 http://www.akademiabaru.com/doc/ARMSV27_N1_P1_10.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Ahmad, Mani Malam Abdul Aziz, Mohd Azoddein Mior Ahmad Khushairi, Mohd Zahari Mazrul Nizam, Abu Seman Mohammad Saedi, Jami Faten Ahada, Mohd Azli Assessment of Sulfide Concentration Effects in a Micro-Oxygenated Condition on the Growth and Removal by Pseudomonas putida (ATCC 49128)
description	Micro-oxygenation has recently being gaining popularity as a successful biological sulfide oxidation technique from inflicted wastewater. In this study the application of orbital shaker as an alternative digester for sulfi de bioxidation was experimented using Pseudomonas putida (ATCC 49128), under an oxygen tension or micro-oxygenated condition. Growth and sulfide reduction efficiency was measured spectrophotometrically under optimum physical conditions of pH, temperature, acclimatization time and agitation. Sulfide reduction was overwhelmingly recorded at three different sulfide loading rates of 200mM S2- L-1 d-1, 300mM S2 L-d-1 and 500mM S2-L-d-1 with corresponding appreciable cell growth measured at OD600nm. The obtained results indicated that it was possible to realized sulfide removal efficiency of 96% to 100% within 24hrs, as well 45% to 70% within the first 6hrs of inoculation, with an overwhelming removal of 100% after 18hrs in 200mM and 300mM. On the other hand, the corresponding growth was 3.00 OD600nm, 2.824 OD600nm and 2.456 OD600nm in 500pmm, 200ppm and 300ppm concentration respectively. Based on this finding, it was clear that this inoculum can be employed to treat sulfide contaminated wastewater even at higher range under micro-oxygena ted environmental condition.
format	Article
author	Ahmad, Mani Malam Abdul Aziz, Mohd Azoddein Mior Ahmad Khushairi, Mohd Zahari Mazrul Nizam, Abu Seman Mohammad Saedi, Jami Faten Ahada, Mohd Azli
author_facet	Ahmad, Mani Malam Abdul Aziz, Mohd Azoddein Mior Ahmad Khushairi, Mohd Zahari Mazrul Nizam, Abu Seman Mohammad Saedi, Jami Faten Ahada, Mohd Azli
author_sort	Ahmad, Mani Malam
title	Assessment of Sulfide Concentration Effects in a Micro-Oxygenated Condition on the Growth and Removal by Pseudomonas putida (ATCC 49128)
title_short	Assessment of Sulfide Concentration Effects in a Micro-Oxygenated Condition on the Growth and Removal by Pseudomonas putida (ATCC 49128)
title_full	Assessment of Sulfide Concentration Effects in a Micro-Oxygenated Condition on the Growth and Removal by Pseudomonas putida (ATCC 49128)
title_fullStr	Assessment of Sulfide Concentration Effects in a Micro-Oxygenated Condition on the Growth and Removal by Pseudomonas putida (ATCC 49128)
title_full_unstemmed	Assessment of Sulfide Concentration Effects in a Micro-Oxygenated Condition on the Growth and Removal by Pseudomonas putida (ATCC 49128)
title_sort	assessment of sulfide concentration effects in a micro-oxygenated condition on the growth and removal by pseudomonas putida (atcc 49128)
publisher	Penerbit Akademia Baru
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/17566/ http://umpir.ump.edu.my/id/eprint/17566/ http://umpir.ump.edu.my/id/eprint/17566/1/fkksa-2016-mazrul-Assessment%20of%20sulfide%20concentration%20effects.pdf
first_indexed	2023-09-18T22:24:20Z
last_indexed	2023-09-18T22:24:20Z
_version_	1777415865878183936

Assessment of Sulfide Concentration Effects in a Micro-Oxygenated Condition on the Growth and Removal by Pseudomonas putida (ATCC 49128)

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