Bacteria Isolation from Landfill for Production of Industrial Enzymes for Waste Degradation

Background/Objectives: The purpose of this study is to isolate the potential bacteria for the production of industrial enzyme cellulose and xylanase for waste degradation. Methods/Statistical Analysis: The bacteria were isolated from three different sources (landfill surface, 10 cm depth soil and mo...

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Main Authors:	N., Masngut, S., Manap, S., Shaarani, Rohaida, Che Man
Format:	Article
Language:	English English
Published:	Indian Society of Education and Environment 2017
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/17512/ http://umpir.ump.edu.my/id/eprint/17512/ http://umpir.ump.edu.my/id/eprint/17512/ http://umpir.ump.edu.my/id/eprint/17512/1/Bacteria%20Isolation%20from%20Landfill%20for%20Production%20of%20Industrial%20Enzymes%20for%20Waste%20Degradation.pdf http://umpir.ump.edu.my/id/eprint/17512/2/Bacteria%20Isolation%20from%20Landfill%20for%20Production%20of%20Industrial%20Enzymes%20for%20Waste%20Degradation%201.pdf

id	ump-17512
recordtype	eprints
spelling	ump-175122018-04-18T03:18:56Z http://umpir.ump.edu.my/id/eprint/17512/ Bacteria Isolation from Landfill for Production of Industrial Enzymes for Waste Degradation N., Masngut S., Manap S., Shaarani Rohaida, Che Man TP Chemical technology Background/Objectives: The purpose of this study is to isolate the potential bacteria for the production of industrial enzyme cellulose and xylanase for waste degradation. Methods/Statistical Analysis: The bacteria were isolated from three different sources (landfill surface, 10 cm depth soil and moist soil) collected from Sungai Ikan Landfill, Kuala Terengganu, Malaysia using serial dilution and spread plate technique. Primarily screening for the enzyme production was carried out. The bacteria isolates which shows a good clearing zone diameter in ranged 2.6-8.8 cm were selected for further quantitative screening for cellulase and xylanase production by performed submerged fermentation using Carboxymethyl Cellulose (CMC) and xylan as a substrate. Findings: A total of 130 potential bacteria were isolated on Nutrient Agar (NA). Only 50 and 30 of the isolates exhibited cellulase and xylanase, respectively, by showing clear zone on CMC and Xylan agar plates after stained with iodine dye. From the observation of the quantitative screening of enzyme activities, B6.2 isolate exhibited the highest cellulase activity (endoglucanase19.797 U/m Land FPase 7.384 U/mL) and M29 isolate exhibited the highest activity of xylanase (5399 U/mL). Overall, all isolates showed good cellulase and xylanse activity ranged between 0.491-19.797 U/mL and 2075-5399 U/mL, respectively. Application/Improvements: For future study, the enzyme produce will be used to degrade waste into fermentable sugars which used as a feedstock for bioethanol production. Indian Society of Education and Environment 2017-02 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/17512/1/Bacteria%20Isolation%20from%20Landfill%20for%20Production%20of%20Industrial%20Enzymes%20for%20Waste%20Degradation.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/17512/2/Bacteria%20Isolation%20from%20Landfill%20for%20Production%20of%20Industrial%20Enzymes%20for%20Waste%20Degradation%201.pdf N., Masngut and S., Manap and S., Shaarani and Rohaida, Che Man (2017) Bacteria Isolation from Landfill for Production of Industrial Enzymes for Waste Degradation. Indian Journal of Science and Technology, 10 (7). pp. 1-5. ISSN 0974-6846(print); 0974-5645(Online) http://dx.doi.org/10.17485/ijst%2F2017%2Fv10i7%2F111224 DOI: 10.17485/ijst/2017/v10i7/111224
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology N., Masngut S., Manap S., Shaarani Rohaida, Che Man Bacteria Isolation from Landfill for Production of Industrial Enzymes for Waste Degradation
description	Background/Objectives: The purpose of this study is to isolate the potential bacteria for the production of industrial enzyme cellulose and xylanase for waste degradation. Methods/Statistical Analysis: The bacteria were isolated from three different sources (landfill surface, 10 cm depth soil and moist soil) collected from Sungai Ikan Landfill, Kuala Terengganu, Malaysia using serial dilution and spread plate technique. Primarily screening for the enzyme production was carried out. The bacteria isolates which shows a good clearing zone diameter in ranged 2.6-8.8 cm were selected for further quantitative screening for cellulase and xylanase production by performed submerged fermentation using Carboxymethyl Cellulose (CMC) and xylan as a substrate. Findings: A total of 130 potential bacteria were isolated on Nutrient Agar (NA). Only 50 and 30 of the isolates exhibited cellulase and xylanase, respectively, by showing clear zone on CMC and Xylan agar plates after stained with iodine dye. From the observation of the quantitative screening of enzyme activities, B6.2 isolate exhibited the highest cellulase activity (endoglucanase19.797 U/m Land FPase 7.384 U/mL) and M29 isolate exhibited the highest activity of xylanase (5399 U/mL). Overall, all isolates showed good cellulase and xylanse activity ranged between 0.491-19.797 U/mL and 2075-5399 U/mL, respectively. Application/Improvements: For future study, the enzyme produce will be used to degrade waste into fermentable sugars which used as a feedstock for bioethanol production.
format	Article
author	N., Masngut S., Manap S., Shaarani Rohaida, Che Man
author_facet	N., Masngut S., Manap S., Shaarani Rohaida, Che Man
author_sort	N., Masngut
title	Bacteria Isolation from Landfill for Production of Industrial Enzymes for Waste Degradation
title_short	Bacteria Isolation from Landfill for Production of Industrial Enzymes for Waste Degradation
title_full	Bacteria Isolation from Landfill for Production of Industrial Enzymes for Waste Degradation
title_fullStr	Bacteria Isolation from Landfill for Production of Industrial Enzymes for Waste Degradation
title_full_unstemmed	Bacteria Isolation from Landfill for Production of Industrial Enzymes for Waste Degradation
title_sort	bacteria isolation from landfill for production of industrial enzymes for waste degradation
publisher	Indian Society of Education and Environment
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/17512/ http://umpir.ump.edu.my/id/eprint/17512/ http://umpir.ump.edu.my/id/eprint/17512/ http://umpir.ump.edu.my/id/eprint/17512/1/Bacteria%20Isolation%20from%20Landfill%20for%20Production%20of%20Industrial%20Enzymes%20for%20Waste%20Degradation.pdf http://umpir.ump.edu.my/id/eprint/17512/2/Bacteria%20Isolation%20from%20Landfill%20for%20Production%20of%20Industrial%20Enzymes%20for%20Waste%20Degradation%201.pdf
first_indexed	2023-09-18T22:24:14Z
last_indexed	2023-09-18T22:24:14Z
_version_	1777415858861113344

Bacteria Isolation from Landfill for Production of Industrial Enzymes for Waste Degradation

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