Factors Affecting Enzymatic Hydrolysis from Pretreated Fibre Pressed Oil Palm Frond Using Sacchariseb C6

In this study, fibre pressed oil palm frond (FPOPF) was introduced as the raw material for the production of glucose using Sacchariseb C6 to maximise the utilisation of oil palm waste. Sacchariseb C6 is a commercial cellulase blended enzyme from Advanced Enzymes Technology. In order to achieve maxim...

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Main Authors:	Fatin Syazwana, Hashim, Wan Mohd Hafizuddin, Wan Yussof, Mah, Kah Hong, Nurul Fatihah, Mahamad Roli, Syed Mohd Saufi, Tuan Chik, Mazrul Nizam, Abu Seman, Abdul Wahab, Mohammad
Format:	Article
Language:	English
Published:	Penerbit Universiti Sains Malaysia 2017
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/17499/ http://umpir.ump.edu.my/id/eprint/17499/ http://umpir.ump.edu.my/id/eprint/17499/ http://umpir.ump.edu.my/id/eprint/17499/1/fkksa-2017-hafizuddin-Factors%20Affecting%20Enzymatic%20Hydrolysis%20from%20Pretreated.pdf

id	ump-17499
recordtype	eprints
spelling	ump-174992019-10-30T07:58:19Z http://umpir.ump.edu.my/id/eprint/17499/ Factors Affecting Enzymatic Hydrolysis from Pretreated Fibre Pressed Oil Palm Frond Using Sacchariseb C6 Fatin Syazwana, Hashim Wan Mohd Hafizuddin, Wan Yussof Mah, Kah Hong Nurul Fatihah, Mahamad Roli Syed Mohd Saufi, Tuan Chik Mazrul Nizam, Abu Seman Abdul Wahab, Mohammad TP Chemical technology In this study, fibre pressed oil palm frond (FPOPF) was introduced as the raw material for the production of glucose using Sacchariseb C6 to maximise the utilisation of oil palm waste. Sacchariseb C6 is a commercial cellulase blended enzyme from Advanced Enzymes Technology. In order to achieve maximum glucose production, a factorial analysis 25–1 using response surface methodology (RSM) was employed to screen the best enzymatic hydrolysis condition by varying the parameters such as agitation speed, enzyme loading, glucan loading, temperature and hydrolysis time. FPOPF was treated with 4.42% (w/v) of sodium hydroxide at 100°C prior to the enzymatic hydrolysis. Raw FPOPF consists of 40.7% glucan, 26.1% xylan, 26.2% lignin, 1.8% ash and 4.5% extractives. On the other hand, pretreated FPOPF consists of 61.4% glucan, 20.4% xylan, 13.3% lignin, 1.3% ash and 0.3% extractives. From this study, it was found that the best enzymatic hydrolysis condition yielded 33.01 ± 0.73 g/L of glucose when performed at 200 rpm of agitation speed, 60 FPU/mL of enzyme loading, 4% (w/w) of glucan loading, temperature at 55°C and 72 h of reaction time. The model obtained from RSM was significant with p-value <0.0001. It is suggested that this model had a maximum point which is likely to be the optimum point and possible for the optimisation process. Penerbit Universiti Sains Malaysia 2017 Article PeerReviewed application/pdf en cc_by http://umpir.ump.edu.my/id/eprint/17499/1/fkksa-2017-hafizuddin-Factors%20Affecting%20Enzymatic%20Hydrolysis%20from%20Pretreated.pdf Fatin Syazwana, Hashim and Wan Mohd Hafizuddin, Wan Yussof and Mah, Kah Hong and Nurul Fatihah, Mahamad Roli and Syed Mohd Saufi, Tuan Chik and Mazrul Nizam, Abu Seman and Abdul Wahab, Mohammad (2017) Factors Affecting Enzymatic Hydrolysis from Pretreated Fibre Pressed Oil Palm Frond Using Sacchariseb C6. Journal of Physical Science, 28 (Supp.1). pp. 281-295. ISSN 1675-3402 (print); 2180-4230 (online) https://doi.org/10.21315/jps2017.28.s1.19 doi: 10.21315/jps2017.28.s1.19
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Fatin Syazwana, Hashim Wan Mohd Hafizuddin, Wan Yussof Mah, Kah Hong Nurul Fatihah, Mahamad Roli Syed Mohd Saufi, Tuan Chik Mazrul Nizam, Abu Seman Abdul Wahab, Mohammad Factors Affecting Enzymatic Hydrolysis from Pretreated Fibre Pressed Oil Palm Frond Using Sacchariseb C6
description	In this study, fibre pressed oil palm frond (FPOPF) was introduced as the raw material for the production of glucose using Sacchariseb C6 to maximise the utilisation of oil palm waste. Sacchariseb C6 is a commercial cellulase blended enzyme from Advanced Enzymes Technology. In order to achieve maximum glucose production, a factorial analysis 25–1 using response surface methodology (RSM) was employed to screen the best enzymatic hydrolysis condition by varying the parameters such as agitation speed, enzyme loading, glucan loading, temperature and hydrolysis time. FPOPF was treated with 4.42% (w/v) of sodium hydroxide at 100°C prior to the enzymatic hydrolysis. Raw FPOPF consists of 40.7% glucan, 26.1% xylan, 26.2% lignin, 1.8% ash and 4.5% extractives. On the other hand, pretreated FPOPF consists of 61.4% glucan, 20.4% xylan, 13.3% lignin, 1.3% ash and 0.3% extractives. From this study, it was found that the best enzymatic hydrolysis condition yielded 33.01 ± 0.73 g/L of glucose when performed at 200 rpm of agitation speed, 60 FPU/mL of enzyme loading, 4% (w/w) of glucan loading, temperature at 55°C and 72 h of reaction time. The model obtained from RSM was significant with p-value <0.0001. It is suggested that this model had a maximum point which is likely to be the optimum point and possible for the optimisation process.
format	Article
author	Fatin Syazwana, Hashim Wan Mohd Hafizuddin, Wan Yussof Mah, Kah Hong Nurul Fatihah, Mahamad Roli Syed Mohd Saufi, Tuan Chik Mazrul Nizam, Abu Seman Abdul Wahab, Mohammad
author_facet	Fatin Syazwana, Hashim Wan Mohd Hafizuddin, Wan Yussof Mah, Kah Hong Nurul Fatihah, Mahamad Roli Syed Mohd Saufi, Tuan Chik Mazrul Nizam, Abu Seman Abdul Wahab, Mohammad
author_sort	Fatin Syazwana, Hashim
title	Factors Affecting Enzymatic Hydrolysis from Pretreated Fibre Pressed Oil Palm Frond Using Sacchariseb C6
title_short	Factors Affecting Enzymatic Hydrolysis from Pretreated Fibre Pressed Oil Palm Frond Using Sacchariseb C6
title_full	Factors Affecting Enzymatic Hydrolysis from Pretreated Fibre Pressed Oil Palm Frond Using Sacchariseb C6
title_fullStr	Factors Affecting Enzymatic Hydrolysis from Pretreated Fibre Pressed Oil Palm Frond Using Sacchariseb C6
title_full_unstemmed	Factors Affecting Enzymatic Hydrolysis from Pretreated Fibre Pressed Oil Palm Frond Using Sacchariseb C6
title_sort	factors affecting enzymatic hydrolysis from pretreated fibre pressed oil palm frond using sacchariseb c6
publisher	Penerbit Universiti Sains Malaysia
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/17499/ http://umpir.ump.edu.my/id/eprint/17499/ http://umpir.ump.edu.my/id/eprint/17499/ http://umpir.ump.edu.my/id/eprint/17499/1/fkksa-2017-hafizuddin-Factors%20Affecting%20Enzymatic%20Hydrolysis%20from%20Pretreated.pdf
first_indexed	2023-09-18T22:24:12Z
last_indexed	2023-09-18T22:24:12Z
_version_	1777415857167663104

Factors Affecting Enzymatic Hydrolysis from Pretreated Fibre Pressed Oil Palm Frond Using Sacchariseb C6

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