Characterization of α-Cyclodextrin Glucanotransferase from Bacillus licheniformis

Objectives: Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme, which undergoes cyclization reaction to converts starch into Cyclodextrin (CD). Due to their potential properties, CDs had been discovered to have numerous application in food industries, pharmaceutical, agr...

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Main Authors:	N., Jamil, Rohaida, Che Man, Shalyda, Md Shaarani, Siti Zubaidah, Sulaiman, Siti Kholijah, Abdul Mudalip, Zatul Iffah, Mohd Arshad
Format:	Article
Language:	English
Published:	Informatics Publishing Limited 2017
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/17468/ http://umpir.ump.edu.my/id/eprint/17468/ http://umpir.ump.edu.my/id/eprint/17468/ http://umpir.ump.edu.my/id/eprint/17468/1/fkksa-2017-rohaida-Characterization%20of%20%CE%B1-Cyclodextrin.pdf

id	ump-17468
recordtype	eprints
spelling	ump-174682017-08-16T03:00:28Z http://umpir.ump.edu.my/id/eprint/17468/ Characterization of α-Cyclodextrin Glucanotransferase from Bacillus licheniformis N., Jamil Rohaida, Che Man Shalyda, Md Shaarani Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Zatul Iffah, Mohd Arshad TP Chemical technology Objectives: Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme, which undergoes cyclization reaction to converts starch into Cyclodextrin (CD). Due to their potential properties, CDs had been discovered to have numerous application in food industries, pharmaceutical, agriculture and also environmental engineering. To improve the production of cyclodextrin (CD) in the future, characterization of α-CGTase on the effect of operating conditions was investigated. Methods/Statistical Analysis: The α-CGTase from Bacillus licheniformis was characterized by examining their cyclization activity. The enzymatic activity of the enzyme towards the temperature and pH was determined by methyl orange method. Findings: The enzyme was estimated to be 70 kDa by the gel electrophoresis. The cyclization activity of α-CGTase was highest at a temperature of 40°C and pH 6.0. The α-CGTase enzyme was able to extend its thermostability up to 60°C with pH stability between pH 6.0 and pH 8.0. Application/Improvements: High production of CD is expected to obtain by using the optimal conditions which in turn may be beneficial for the industrial purpose. Informatics Publishing Limited 2017 Article PeerReviewed application/pdf en cc_by http://umpir.ump.edu.my/id/eprint/17468/1/fkksa-2017-rohaida-Characterization%20of%20%CE%B1-Cyclodextrin.pdf N., Jamil and Rohaida, Che Man and Shalyda, Md Shaarani and Siti Zubaidah, Sulaiman and Siti Kholijah, Abdul Mudalip and Zatul Iffah, Mohd Arshad (2017) Characterization of α-Cyclodextrin Glucanotransferase from Bacillus licheniformis. Indian Journal of Science and Technology, 10 (7). pp. 1-5. ISSN 0974-6846 (Print); 0974-5645 (Online) http://www.indjst.org/index.php/indjst/article/view/111220 DOI: 10.17485/ijst/2017/v10i7/111220
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology N., Jamil Rohaida, Che Man Shalyda, Md Shaarani Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Zatul Iffah, Mohd Arshad Characterization of α-Cyclodextrin Glucanotransferase from Bacillus licheniformis
description	Objectives: Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme, which undergoes cyclization reaction to converts starch into Cyclodextrin (CD). Due to their potential properties, CDs had been discovered to have numerous application in food industries, pharmaceutical, agriculture and also environmental engineering. To improve the production of cyclodextrin (CD) in the future, characterization of α-CGTase on the effect of operating conditions was investigated. Methods/Statistical Analysis: The α-CGTase from Bacillus licheniformis was characterized by examining their cyclization activity. The enzymatic activity of the enzyme towards the temperature and pH was determined by methyl orange method. Findings: The enzyme was estimated to be 70 kDa by the gel electrophoresis. The cyclization activity of α-CGTase was highest at a temperature of 40°C and pH 6.0. The α-CGTase enzyme was able to extend its thermostability up to 60°C with pH stability between pH 6.0 and pH 8.0. Application/Improvements: High production of CD is expected to obtain by using the optimal conditions which in turn may be beneficial for the industrial purpose.
format	Article
author	N., Jamil Rohaida, Che Man Shalyda, Md Shaarani Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Zatul Iffah, Mohd Arshad
author_facet	N., Jamil Rohaida, Che Man Shalyda, Md Shaarani Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Zatul Iffah, Mohd Arshad
author_sort	N., Jamil
title	Characterization of α-Cyclodextrin Glucanotransferase from Bacillus licheniformis
title_short	Characterization of α-Cyclodextrin Glucanotransferase from Bacillus licheniformis
title_full	Characterization of α-Cyclodextrin Glucanotransferase from Bacillus licheniformis
title_fullStr	Characterization of α-Cyclodextrin Glucanotransferase from Bacillus licheniformis
title_full_unstemmed	Characterization of α-Cyclodextrin Glucanotransferase from Bacillus licheniformis
title_sort	characterization of α-cyclodextrin glucanotransferase from bacillus licheniformis
publisher	Informatics Publishing Limited
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/17468/ http://umpir.ump.edu.my/id/eprint/17468/ http://umpir.ump.edu.my/id/eprint/17468/ http://umpir.ump.edu.my/id/eprint/17468/1/fkksa-2017-rohaida-Characterization%20of%20%CE%B1-Cyclodextrin.pdf
first_indexed	2023-09-18T22:24:08Z
last_indexed	2023-09-18T22:24:08Z
_version_	1777415852863258624

Characterization of α-Cyclodextrin Glucanotransferase from Bacillus licheniformis

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