Effect of Time, Inoculum size (%) and Mass Substrate on succinic acid production from glycerol residue using immobilized Escherichia Coli

Effect of Time, Inoculum size (%) and Mass Substrate on succinic acid production from glycerol residue using immobilized Escherichia Coli

The study on succinic acid production from glycerol residue using immobilized Escherichia coli in a simple fermentation process has shown a potentially low-cost production process. In this research, the bacterial cells were prepared by the entrapment method. This method was chosen since it was repor...

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Main Authors: Nik Nor Aziati, Abdul Aziz, Mimi Sakinah, A. M.
Format: Article
Language:English
Published: AENSI Publishing 2016
Subjects:
QD Chemistry
Online Access:http://umpir.ump.edu.my/id/eprint/17285/
http://umpir.ump.edu.my/id/eprint/17285/
http://umpir.ump.edu.my/id/eprint/17285/1/ftech-2016-mimi-Effect%20of%20Time%2C%20Inoculum%20size.pdf
id ump-17285
recordtype eprints
spelling ump-172852018-01-17T06:44:05Z http://umpir.ump.edu.my/id/eprint/17285/ Effect of Time, Inoculum size (%) and Mass Substrate on succinic acid production from glycerol residue using immobilized Escherichia Coli Nik Nor Aziati, Abdul Aziz Mimi Sakinah, A. M. QD Chemistry The study on succinic acid production from glycerol residue using immobilized Escherichia coli in a simple fermentation process has shown a potentially low-cost production process. In this research, the bacterial cells were prepared by the entrapment method. This method was chosen since it was reported to be an effective method for large scale production of succinic acid. The effects of different times, inoculum size, and mass substrate values on succinic acid production were studied. Batch culture technique was employed to grow the Escherichia coli and the entrapment method for the immobilization of the cells. The optimum condition was observed as 30 g mass substrate (117.99 g/L succinic acid production), inoculum size 20 % working volume (102.30 g/L succinic acid production),and time of 4 h (110.20 g/L succinic acid production). This process condition gave the maximum succinic acid concentration. Preliminary characterization of the raw material was done by using the High-Performance Liquid Chromatography and Fourier Transform Infrared Spectrometry (FTIR). The results were then compared to the raw material (glycerol residue), pretreated glycerol (after pretreatment) and succinic acid concentrations. The effects of mass substrate, inoculums densities, and time were significant on the succinic acid production by immobilized cells. AENSI Publishing 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/17285/1/ftech-2016-mimi-Effect%20of%20Time%2C%20Inoculum%20size.pdf Nik Nor Aziati, Abdul Aziz and Mimi Sakinah, A. M. (2016) Effect of Time, Inoculum size (%) and Mass Substrate on succinic acid production from glycerol residue using immobilized Escherichia Coli. Australian Journal of Basic and Applied Sciences, 10 (17 Sp.). pp. 75-83. ISSN 1991-8178 http://www.ajbasweb.com/old/ajbas/2016/Special%20ICCEIB/75-83.pdf
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
Nik Nor Aziati, Abdul Aziz
Mimi Sakinah, A. M.
Effect of Time, Inoculum size (%) and Mass Substrate on succinic acid production from glycerol residue using immobilized Escherichia Coli
description The study on succinic acid production from glycerol residue using immobilized Escherichia coli in a simple fermentation process has shown a potentially low-cost production process. In this research, the bacterial cells were prepared by the entrapment method. This method was chosen since it was reported to be an effective method for large scale production of succinic acid. The effects of different times, inoculum size, and mass substrate values on succinic acid production were studied. Batch culture technique was employed to grow the Escherichia coli and the entrapment method for the immobilization of the cells. The optimum condition was observed as 30 g mass substrate (117.99 g/L succinic acid production), inoculum size 20 % working volume (102.30 g/L succinic acid production),and time of 4 h (110.20 g/L succinic acid production). This process condition gave the maximum succinic acid concentration. Preliminary characterization of the raw material was done by using the High-Performance Liquid Chromatography and Fourier Transform Infrared Spectrometry (FTIR). The results were then compared to the raw material (glycerol residue), pretreated glycerol (after pretreatment) and succinic acid concentrations. The effects of mass substrate, inoculums densities, and time were significant on the succinic acid production by immobilized cells.
format Article
author Nik Nor Aziati, Abdul Aziz
Mimi Sakinah, A. M.
author_facet Nik Nor Aziati, Abdul Aziz
Mimi Sakinah, A. M.
author_sort Nik Nor Aziati, Abdul Aziz
title Effect of Time, Inoculum size (%) and Mass Substrate on succinic acid production from glycerol residue using immobilized Escherichia Coli
title_short Effect of Time, Inoculum size (%) and Mass Substrate on succinic acid production from glycerol residue using immobilized Escherichia Coli
title_full Effect of Time, Inoculum size (%) and Mass Substrate on succinic acid production from glycerol residue using immobilized Escherichia Coli
title_fullStr Effect of Time, Inoculum size (%) and Mass Substrate on succinic acid production from glycerol residue using immobilized Escherichia Coli
title_full_unstemmed Effect of Time, Inoculum size (%) and Mass Substrate on succinic acid production from glycerol residue using immobilized Escherichia Coli
title_sort effect of time, inoculum size (%) and mass substrate on succinic acid production from glycerol residue using immobilized escherichia coli
publisher AENSI Publishing
publishDate 2016
url http://umpir.ump.edu.my/id/eprint/17285/
http://umpir.ump.edu.my/id/eprint/17285/
http://umpir.ump.edu.my/id/eprint/17285/1/ftech-2016-mimi-Effect%20of%20Time%2C%20Inoculum%20size.pdf
first_indexed 2023-09-18T22:23:45Z
last_indexed 2023-09-18T22:23:45Z
_version_ 1777415828770127872

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