Synthetic Curcumin Derivative DK1 Possessed G2/M Arrest and Induced Apoptosis Through Accumulation of Intracellular ROS in MCF-7 Breast Cancer Cells
Curcumin is a lead compound of the rhizomes of Curcuma longa and possess a broad range of pharmacological activities. Chemically, curcumin is 1,3-dicarbonyl class of compound, which exhibits keto-enol tautomerism. Despite of its strong biological properties, curcumin has yet been recommended as a th...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BioMed Central
2017
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/17120/ http://umpir.ump.edu.my/id/eprint/17120/ http://umpir.ump.edu.my/id/eprint/17120/ http://umpir.ump.edu.my/id/eprint/17120/1/fist-2017-nadeem-Synthetic%20curcumin%20derivative%20DK1.pdf |
| Summary: | Curcumin is a lead compound of the rhizomes of Curcuma longa and possess a broad range of pharmacological activities. Chemically, curcumin is 1,3-dicarbonyl class of compound, which exhibits keto-enol tautomerism. Despite of its strong biological properties, curcumin has yet been recommended as a therapeutic agent because of its poor bioavailability. A curcumin derivative (Z)-3-hydroxy-1-(2-hydroxyphenyl)-3-phenylprop-2-en-1-one (DK1) was synthesized and its cytotoxicity was tested on breast cancer cell MCF-7 and normal cell MCF-10A using MTT assay. Meanwhile, cell cycle regulation and apoptosis on MCF-7 cell were evaluated using flow cytometry. Regulation of cell cycle and apoptosis related genes expression was investigated by quantitative real time polymerase chain reaction (qRT-PCR), western blot and caspases activity analyses. Activation of oxidative stress on MCF-7 were evaluated by measuring ROS and GSH levels. |
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