Statistical Modeling and Optimization of Enzymatic Pretreatment of Empty Fruit Bunches with Laccase Enzyme

Laccase enzyme was used as a pretreatment agent to delignify empty fruit bunches (EFB) for sugar production. The degree of delignification of the biomass was assessed directly by the percentage of pre-pretreatment weight loss (%) after pretreatment and indirectly by the amount of total sugar produce...

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Main Authors:	Ishmael, Ukaegbu Chinonso, Rashid, Shah Samiur, Jaya Vejayan, Palliah, Mohd Fazli, Farida Asras, Sharifah Suhaiza, Nik Wan Ahmad, Ayodele, Bamidele V.
Format:	Article
Language:	English
Published:	BioResources 2016
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/16964/ http://umpir.ump.edu.my/id/eprint/16964/ http://umpir.ump.edu.my/id/eprint/16964/1/2016-4424.pdf

id	ump-16964
recordtype	eprints
spelling	ump-169642019-08-28T07:04:19Z http://umpir.ump.edu.my/id/eprint/16964/ Statistical Modeling and Optimization of Enzymatic Pretreatment of Empty Fruit Bunches with Laccase Enzyme Ishmael, Ukaegbu Chinonso Rashid, Shah Samiur Jaya Vejayan, Palliah Mohd Fazli, Farida Asras Sharifah Suhaiza, Nik Wan Ahmad Ayodele, Bamidele V. TP Chemical technology Laccase enzyme was used as a pretreatment agent to delignify empty fruit bunches (EFB) for sugar production. The degree of delignification of the biomass was assessed directly by the percentage of pre-pretreatment weight loss (%) after pretreatment and indirectly by the amount of total sugar produced after saccharification of the pretreated biomass with cellulase enzymes. Process parameters such as pretreatment time, temperature, enzyme concentration, substrate concentration, pH, and substrate size were studied using a one-factor-at-a-time (OFAT) analysis. The combined effect of temperature and pH on the pretreatment was studied using the face-centered central composite design (FCCCD) of response surface methodology (RSM). The optimized conditions for EFB pretreatment using laccase enzyme were achieved as follows: sample size, 2 mm; temperature, 25 °C; time, 4 h; substrate concentration, 5% (w/v); pH 5; and enzyme concentration, 20 IU/g of EFB. Although higher pretreatment was achieved with substrates of 1 mm size and at a temperature of 35 °C, these conditions were not considered energetically sustainable because of the need for energy during milling for sample size reduction and energy for temperature maintenance at 35 °C. BioResources 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/16964/1/2016-4424.pdf Ishmael, Ukaegbu Chinonso and Rashid, Shah Samiur and Jaya Vejayan, Palliah and Mohd Fazli, Farida Asras and Sharifah Suhaiza, Nik Wan Ahmad and Ayodele, Bamidele V. (2016) Statistical Modeling and Optimization of Enzymatic Pretreatment of Empty Fruit Bunches with Laccase Enzyme. BioResources , 11 (2). pp. 5013-5032. ISSN 1930-2126 http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_2_5013_Ishmael_Modeling_Optimization_Enzymatic_Pretreatment
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Ishmael, Ukaegbu Chinonso Rashid, Shah Samiur Jaya Vejayan, Palliah Mohd Fazli, Farida Asras Sharifah Suhaiza, Nik Wan Ahmad Ayodele, Bamidele V. Statistical Modeling and Optimization of Enzymatic Pretreatment of Empty Fruit Bunches with Laccase Enzyme
description	Laccase enzyme was used as a pretreatment agent to delignify empty fruit bunches (EFB) for sugar production. The degree of delignification of the biomass was assessed directly by the percentage of pre-pretreatment weight loss (%) after pretreatment and indirectly by the amount of total sugar produced after saccharification of the pretreated biomass with cellulase enzymes. Process parameters such as pretreatment time, temperature, enzyme concentration, substrate concentration, pH, and substrate size were studied using a one-factor-at-a-time (OFAT) analysis. The combined effect of temperature and pH on the pretreatment was studied using the face-centered central composite design (FCCCD) of response surface methodology (RSM). The optimized conditions for EFB pretreatment using laccase enzyme were achieved as follows: sample size, 2 mm; temperature, 25 °C; time, 4 h; substrate concentration, 5% (w/v); pH 5; and enzyme concentration, 20 IU/g of EFB. Although higher pretreatment was achieved with substrates of 1 mm size and at a temperature of 35 °C, these conditions were not considered energetically sustainable because of the need for energy during milling for sample size reduction and energy for temperature maintenance at 35 °C.
format	Article
author	Ishmael, Ukaegbu Chinonso Rashid, Shah Samiur Jaya Vejayan, Palliah Mohd Fazli, Farida Asras Sharifah Suhaiza, Nik Wan Ahmad Ayodele, Bamidele V.
author_facet	Ishmael, Ukaegbu Chinonso Rashid, Shah Samiur Jaya Vejayan, Palliah Mohd Fazli, Farida Asras Sharifah Suhaiza, Nik Wan Ahmad Ayodele, Bamidele V.
author_sort	Ishmael, Ukaegbu Chinonso
title	Statistical Modeling and Optimization of Enzymatic Pretreatment of Empty Fruit Bunches with Laccase Enzyme
title_short	Statistical Modeling and Optimization of Enzymatic Pretreatment of Empty Fruit Bunches with Laccase Enzyme
title_full	Statistical Modeling and Optimization of Enzymatic Pretreatment of Empty Fruit Bunches with Laccase Enzyme
title_fullStr	Statistical Modeling and Optimization of Enzymatic Pretreatment of Empty Fruit Bunches with Laccase Enzyme
title_full_unstemmed	Statistical Modeling and Optimization of Enzymatic Pretreatment of Empty Fruit Bunches with Laccase Enzyme
title_sort	statistical modeling and optimization of enzymatic pretreatment of empty fruit bunches with laccase enzyme
publisher	BioResources
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/16964/ http://umpir.ump.edu.my/id/eprint/16964/ http://umpir.ump.edu.my/id/eprint/16964/1/2016-4424.pdf
first_indexed	2023-09-18T22:23:05Z
last_indexed	2023-09-18T22:23:05Z
_version_	1777415787254906880

Statistical Modeling and Optimization of Enzymatic Pretreatment of Empty Fruit Bunches with Laccase Enzyme

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