Dual Regulation of Cell Death and Cell Survival upon Induction of Cellular Stress by Isopimara-7,15-Dien-19-Oic Acid in Cervical Cancer, HeLa Cells In vitro

Dual Regulation of Cell Death and Cell Survival upon Induction of Cellular Stress by Isopimara-7,15-Dien-19-Oic Acid in Cervical Cancer, HeLa Cells In vitro

The Fritillaria imperialis is an ornamental flower that can be found in various parts of the world including Iraq, Afghanistan, Pakistan, and the Himalayas. The use of this plant as traditional remedy is widely known. This study aims to unveil the anti-cancer potentials of Isopimara-7,15-Dien-19-Oic...

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Main Authors: Nadiah, Abu, Swee, K. Yeap, Ahmad Z., Mat Pauzi, Akhtar, Muhammad Nadeem, Nur R., Zamberi, Jamil, Ismail, Seema, Zareen, Noorjahan Banu, Alitheen
Format: Article
Language:English
Published: Frontiers Media S.A. 2016
Subjects:
QR Microbiology
Online Access:http://umpir.ump.edu.my/id/eprint/16808/
http://umpir.ump.edu.my/id/eprint/16808/
http://umpir.ump.edu.my/id/eprint/16808/
http://umpir.ump.edu.my/id/eprint/16808/1/fist-2016-nadeem-Dual%20Regulation%20of%20Cell%20Death%20and%20Cell%20Survival%20upon8.pdf
id ump-16808
recordtype eprints
spelling ump-168082017-12-26T03:18:27Z http://umpir.ump.edu.my/id/eprint/16808/ Dual Regulation of Cell Death and Cell Survival upon Induction of Cellular Stress by Isopimara-7,15-Dien-19-Oic Acid in Cervical Cancer, HeLa Cells In vitro Nadiah, Abu Swee, K. Yeap Ahmad Z., Mat Pauzi Akhtar, Muhammad Nadeem Nur R., Zamberi Jamil, Ismail Seema, Zareen Noorjahan Banu, Alitheen QR Microbiology The Fritillaria imperialis is an ornamental flower that can be found in various parts of the world including Iraq, Afghanistan, Pakistan, and the Himalayas. The use of this plant as traditional remedy is widely known. This study aims to unveil the anti-cancer potentials of Isopimara-7,15-Dien-19-Oic Acid, extracted from the bulbs of F. imperialis in cervical cancer cell line, HeLa cells. Flow cytometry analysis of cell death, gene expression analysis via cDNA microarray and protein array were performed. Based on the results, Isopimara-7,15-Dien-19-Oic acid simultaneously induced cell death and promoted cell survival. The execution of apoptosis was apparent based on the flow cytometry results and regulation of both pro and anti-apoptotic genes. Additionally, the regulation of anti-oxidant genes were up-regulated especially thioredoxin, glutathione and superoxide dismutase- related genes. Moreover, the treatment also induced the activation of pro-survival heat shock proteins. Collectively, Isopimara-7,15-Dien-19-Oic Acid managed to induce cellular stress in HeLa cells and activate several anti- and pro survival pathways. Frontiers Media S.A. 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/16808/1/fist-2016-nadeem-Dual%20Regulation%20of%20Cell%20Death%20and%20Cell%20Survival%20upon8.pdf Nadiah, Abu and Swee, K. Yeap and Ahmad Z., Mat Pauzi and Akhtar, Muhammad Nadeem and Nur R., Zamberi and Jamil, Ismail and Seema, Zareen and Noorjahan Banu, Alitheen (2016) Dual Regulation of Cell Death and Cell Survival upon Induction of Cellular Stress by Isopimara-7,15-Dien-19-Oic Acid in Cervical Cancer, HeLa Cells In vitro. Frontiers in Pharmacology, 7 (89). pp. 1-11. ISSN 1663-9812 https://doi.org/10.3389/fphar.2016.00089 doi: 10.3389/fphar.2016.00089
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic QR Microbiology
spellingShingle QR Microbiology
Nadiah, Abu
Swee, K. Yeap
Ahmad Z., Mat Pauzi
Akhtar, Muhammad Nadeem
Nur R., Zamberi
Jamil, Ismail
Seema, Zareen
Noorjahan Banu, Alitheen
Dual Regulation of Cell Death and Cell Survival upon Induction of Cellular Stress by Isopimara-7,15-Dien-19-Oic Acid in Cervical Cancer, HeLa Cells In vitro
description The Fritillaria imperialis is an ornamental flower that can be found in various parts of the world including Iraq, Afghanistan, Pakistan, and the Himalayas. The use of this plant as traditional remedy is widely known. This study aims to unveil the anti-cancer potentials of Isopimara-7,15-Dien-19-Oic Acid, extracted from the bulbs of F. imperialis in cervical cancer cell line, HeLa cells. Flow cytometry analysis of cell death, gene expression analysis via cDNA microarray and protein array were performed. Based on the results, Isopimara-7,15-Dien-19-Oic acid simultaneously induced cell death and promoted cell survival. The execution of apoptosis was apparent based on the flow cytometry results and regulation of both pro and anti-apoptotic genes. Additionally, the regulation of anti-oxidant genes were up-regulated especially thioredoxin, glutathione and superoxide dismutase- related genes. Moreover, the treatment also induced the activation of pro-survival heat shock proteins. Collectively, Isopimara-7,15-Dien-19-Oic Acid managed to induce cellular stress in HeLa cells and activate several anti- and pro survival pathways.
format Article
author Nadiah, Abu
Swee, K. Yeap
Ahmad Z., Mat Pauzi
Akhtar, Muhammad Nadeem
Nur R., Zamberi
Jamil, Ismail
Seema, Zareen
Noorjahan Banu, Alitheen
author_facet Nadiah, Abu
Swee, K. Yeap
Ahmad Z., Mat Pauzi
Akhtar, Muhammad Nadeem
Nur R., Zamberi
Jamil, Ismail
Seema, Zareen
Noorjahan Banu, Alitheen
author_sort Nadiah, Abu
title Dual Regulation of Cell Death and Cell Survival upon Induction of Cellular Stress by Isopimara-7,15-Dien-19-Oic Acid in Cervical Cancer, HeLa Cells In vitro
title_short Dual Regulation of Cell Death and Cell Survival upon Induction of Cellular Stress by Isopimara-7,15-Dien-19-Oic Acid in Cervical Cancer, HeLa Cells In vitro
title_full Dual Regulation of Cell Death and Cell Survival upon Induction of Cellular Stress by Isopimara-7,15-Dien-19-Oic Acid in Cervical Cancer, HeLa Cells In vitro
title_fullStr Dual Regulation of Cell Death and Cell Survival upon Induction of Cellular Stress by Isopimara-7,15-Dien-19-Oic Acid in Cervical Cancer, HeLa Cells In vitro
title_full_unstemmed Dual Regulation of Cell Death and Cell Survival upon Induction of Cellular Stress by Isopimara-7,15-Dien-19-Oic Acid in Cervical Cancer, HeLa Cells In vitro
title_sort dual regulation of cell death and cell survival upon induction of cellular stress by isopimara-7,15-dien-19-oic acid in cervical cancer, hela cells in vitro
publisher Frontiers Media S.A.
publishDate 2016
url http://umpir.ump.edu.my/id/eprint/16808/
http://umpir.ump.edu.my/id/eprint/16808/
http://umpir.ump.edu.my/id/eprint/16808/
http://umpir.ump.edu.my/id/eprint/16808/1/fist-2016-nadeem-Dual%20Regulation%20of%20Cell%20Death%20and%20Cell%20Survival%20upon8.pdf
first_indexed 2023-09-18T22:22:48Z
last_indexed 2023-09-18T22:22:48Z
_version_ 1777415769474203648

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