Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors

In order to maximize enzymatic xylan depolymerization while simultaneously purifying the resulting monosaccharide (xylose), different ultrafiltration (UF) membrane reactor configurations were evaluated. Initial results showed that the two hydrolytic enzymes required for complete depolymerization of...

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Main Authors:	Mohd Shafiq, Mohd Sueb, Luo, Jianquan, Meyer, Anne S., Jørgensen, Henning, Pinelo, Manuel
Format:	Article
Language:	English
Published:	Elsevier Ltd 2017
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/16536/ http://umpir.ump.edu.my/id/eprint/16536/ http://umpir.ump.edu.my/id/eprint/16536/ http://umpir.ump.edu.my/id/eprint/16536/1/Impact%20of%20the%20fouling%20mechanism%20on%20enzymatic%20depolymerization%20of%20xylan%20in%20different%20configurations%20of%20membrane%20reactors.pdf

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spelling	ump-165362017-04-25T07:28:25Z http://umpir.ump.edu.my/id/eprint/16536/ Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors Mohd Shafiq, Mohd Sueb Luo, Jianquan Meyer, Anne S. Jørgensen, Henning Pinelo, Manuel TP Chemical technology In order to maximize enzymatic xylan depolymerization while simultaneously purifying the resulting monosaccharide (xylose), different ultrafiltration (UF) membrane reactor configurations were evaluated. Initial results showed that the two hydrolytic enzymes required for complete depolymerization of xylan, endo-1,4-β-xylanase and β-xylosidase, promoted different types of fouling, which had a direct impact on the extent of xylan hydrolysis achieved during reaction. Endo-1,4-β-xylanase generated DP 1–6 xylo-oligomers. These products contributed to partial pore blocking of the 1 kDa polysulfone membrane and caused irreversible flux loss (∼20%). The presence of β-xylosidase could not prevent deposition of xylan and xylooligomers on the UF membrane surface. Mulder’s modelling of the filtration parameters affirmed that this xylan and xylooligosaccharide deposition formed a cake layer on the membrane which hindered enzymatic attack in addition to fouling. Reaction with both enzymes followed by UF was found to be the optimal configuration, providing at least 40% higher xylan hydrolysis than the cascade configuration (involving sequential reaction with each of the enzymes separately) and the simultaneous reaction-filtration with both enzymes, respectively. This study thus confirmed that the reactor configuration has a crucial impact on the performance of both the reaction and the separation process of xylose during enzymatic xylan degradation, and that the type of fouling mechanism varies in response to the type of enzyme treatment. Elsevier Ltd 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/16536/1/Impact%20of%20the%20fouling%20mechanism%20on%20enzymatic%20depolymerization%20of%20xylan%20in%20different%20configurations%20of%20membrane%20reactors.pdf Mohd Shafiq, Mohd Sueb and Luo, Jianquan and Meyer, Anne S. and Jørgensen, Henning and Pinelo, Manuel (2017) Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors. Separation and Purification Technology, 178. pp. 154-162. ISSN 1383-5866 http://doi.org/10.1016/j.seppur.2017.01.038 DOI: 10.1016/j.seppur.2017.01.038
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Mohd Shafiq, Mohd Sueb Luo, Jianquan Meyer, Anne S. Jørgensen, Henning Pinelo, Manuel Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors
description	In order to maximize enzymatic xylan depolymerization while simultaneously purifying the resulting monosaccharide (xylose), different ultrafiltration (UF) membrane reactor configurations were evaluated. Initial results showed that the two hydrolytic enzymes required for complete depolymerization of xylan, endo-1,4-β-xylanase and β-xylosidase, promoted different types of fouling, which had a direct impact on the extent of xylan hydrolysis achieved during reaction. Endo-1,4-β-xylanase generated DP 1–6 xylo-oligomers. These products contributed to partial pore blocking of the 1 kDa polysulfone membrane and caused irreversible flux loss (∼20%). The presence of β-xylosidase could not prevent deposition of xylan and xylooligomers on the UF membrane surface. Mulder’s modelling of the filtration parameters affirmed that this xylan and xylooligosaccharide deposition formed a cake layer on the membrane which hindered enzymatic attack in addition to fouling. Reaction with both enzymes followed by UF was found to be the optimal configuration, providing at least 40% higher xylan hydrolysis than the cascade configuration (involving sequential reaction with each of the enzymes separately) and the simultaneous reaction-filtration with both enzymes, respectively. This study thus confirmed that the reactor configuration has a crucial impact on the performance of both the reaction and the separation process of xylose during enzymatic xylan degradation, and that the type of fouling mechanism varies in response to the type of enzyme treatment.
format	Article
author	Mohd Shafiq, Mohd Sueb Luo, Jianquan Meyer, Anne S. Jørgensen, Henning Pinelo, Manuel
author_facet	Mohd Shafiq, Mohd Sueb Luo, Jianquan Meyer, Anne S. Jørgensen, Henning Pinelo, Manuel
author_sort	Mohd Shafiq, Mohd Sueb
title	Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors
title_short	Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors
title_full	Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors
title_fullStr	Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors
title_full_unstemmed	Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors
title_sort	impact of the fouling mechanism on enzymatic depolymerization of xylan in different configurations of membrane reactors
publisher	Elsevier Ltd
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/16536/ http://umpir.ump.edu.my/id/eprint/16536/ http://umpir.ump.edu.my/id/eprint/16536/ http://umpir.ump.edu.my/id/eprint/16536/1/Impact%20of%20the%20fouling%20mechanism%20on%20enzymatic%20depolymerization%20of%20xylan%20in%20different%20configurations%20of%20membrane%20reactors.pdf
first_indexed	2023-09-18T22:22:17Z
last_indexed	2023-09-18T22:22:17Z
_version_	1777415736885510144

Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors

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