Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations

Continuous removal of ethanol during production by the anaerobic bacterium Zymomonas mobilis mitigated ethanol inhibition. Ethanol inhibited its own production at a concentration of >20 g L−1. Ethanol productivity was enhanced by continuous in situ extraction with water-immiscible organic solvent...

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Main Authors:	Azilah, Ajit, Ahmad Ziad, Sulaiman, Chisti, Yusuf
Format:	Article
Published:	Elsevier Ltd 2017
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/16450/ http://umpir.ump.edu.my/id/eprint/16450/ http://umpir.ump.edu.my/id/eprint/16450/

id	ump-16450
recordtype	eprints
spelling	ump-164502018-01-30T02:58:34Z http://umpir.ump.edu.my/id/eprint/16450/ Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations Azilah, Ajit Ahmad Ziad, Sulaiman Chisti, Yusuf TP Chemical technology Continuous removal of ethanol during production by the anaerobic bacterium Zymomonas mobilis mitigated ethanol inhibition. Ethanol inhibited its own production at a concentration of >20 g L−1. Ethanol productivity was enhanced by continuous in situ extraction with water-immiscible organic solvents. Several solvents were evaluated for biocompatibility with Z. mobilis and the ability to extract ethanol from a batch fermentation. In situ batch extractive fermentations were carried out in a 2-L stirred bioreactor (1:1 volume ratio of solvent and fermentation broth) with an optimal glucose concentration of 150 g L−1, 35 °C, and an agitation speed of 150 rpm. Oleyl alcohol, iso-octadecanol and 2-octyl-1-dodecanol were the three solvents that were most compatible with Z. mobilis. With these solvents, the cell viability relative to control (no solvent) was 1.48 ± 0.40, 1.03 ± 0.18 and 1.05 ± 0.07, respectively, after two days of exposure. All solvents tested improved the final biomass concentration, the glucose consumption and ethanol production relative to control, but iso-octadecanol was clearly the most effective solvent. Using iso-octadecanol, the maximum ethanol concentration of 75 ± 3 g L−1 was attained. This was nearly 1.25-fold that of the control fermentation. For this solvent, the ethanol yield on glucose was 0.485 ± 0.005 g g−1 compared to a yield of 0.468 ± 0.005 g g−1 for the control culture. Elsevier Ltd 2017 Article PeerReviewed Azilah, Ajit and Ahmad Ziad, Sulaiman and Chisti, Yusuf (2017) Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations. Food and Bioproducts Processing, 102. pp. 123-135. ISSN 0960-3085 http://dx.doi.org/10.1016/j.fbp.2016.12.006 DOI: 10.1016/j.fbp.2016.12.006
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
topic	TP Chemical technology
spellingShingle	TP Chemical technology Azilah, Ajit Ahmad Ziad, Sulaiman Chisti, Yusuf Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations
description	Continuous removal of ethanol during production by the anaerobic bacterium Zymomonas mobilis mitigated ethanol inhibition. Ethanol inhibited its own production at a concentration of >20 g L−1. Ethanol productivity was enhanced by continuous in situ extraction with water-immiscible organic solvents. Several solvents were evaluated for biocompatibility with Z. mobilis and the ability to extract ethanol from a batch fermentation. In situ batch extractive fermentations were carried out in a 2-L stirred bioreactor (1:1 volume ratio of solvent and fermentation broth) with an optimal glucose concentration of 150 g L−1, 35 °C, and an agitation speed of 150 rpm. Oleyl alcohol, iso-octadecanol and 2-octyl-1-dodecanol were the three solvents that were most compatible with Z. mobilis. With these solvents, the cell viability relative to control (no solvent) was 1.48 ± 0.40, 1.03 ± 0.18 and 1.05 ± 0.07, respectively, after two days of exposure. All solvents tested improved the final biomass concentration, the glucose consumption and ethanol production relative to control, but iso-octadecanol was clearly the most effective solvent. Using iso-octadecanol, the maximum ethanol concentration of 75 ± 3 g L−1 was attained. This was nearly 1.25-fold that of the control fermentation. For this solvent, the ethanol yield on glucose was 0.485 ± 0.005 g g−1 compared to a yield of 0.468 ± 0.005 g g−1 for the control culture.
format	Article
author	Azilah, Ajit Ahmad Ziad, Sulaiman Chisti, Yusuf
author_facet	Azilah, Ajit Ahmad Ziad, Sulaiman Chisti, Yusuf
author_sort	Azilah, Ajit
title	Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations
title_short	Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations
title_full	Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations
title_fullStr	Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations
title_full_unstemmed	Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations
title_sort	production of bioethanol by zymomonas mobilis in high-gravity extractive fermentations
publisher	Elsevier Ltd
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/16450/ http://umpir.ump.edu.my/id/eprint/16450/ http://umpir.ump.edu.my/id/eprint/16450/
first_indexed	2023-09-18T22:22:08Z
last_indexed	2023-09-18T22:22:08Z
_version_	1777415727195619328

Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations

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