Preliminary Phylogenetic Analysis of Thermophilic Bacteria Producing Protease isolated from Kuantan Malaysia

Thermophilic bacteria grow in relatively high temperature between 50 to 60°C. These organisms can produce specific enzymes that have been used in industrial and biotechnological applications. The basic method to derive a sequence for a uncultured bacteria is to use universal primers against the 16S...

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Main Authors:	Makky, Essam A., Shaiful Zahid, M. Z., Manaf, Almatar, M. M., Yusoff
Format:	Article
Language:	English
Published:	ISSR 2016
Subjects:	QR Microbiology
Online Access:	http://umpir.ump.edu.my/id/eprint/16419/ http://umpir.ump.edu.my/id/eprint/16419/ http://umpir.ump.edu.my/id/eprint/16419/1/final-essam.pdf

id	ump-16419
recordtype	eprints
spelling	ump-164192018-07-27T03:14:26Z http://umpir.ump.edu.my/id/eprint/16419/ Preliminary Phylogenetic Analysis of Thermophilic Bacteria Producing Protease isolated from Kuantan Malaysia Makky, Essam A. Shaiful Zahid, M. Z. Manaf, Almatar M. M., Yusoff QR Microbiology Thermophilic bacteria grow in relatively high temperature between 50 to 60°C. These organisms can produce specific enzymes that have been used in industrial and biotechnological applications. The basic method to derive a sequence for a uncultured bacteria is to use universal primers against the 16S rRNA gene region in a PCR step to increase the amount of DNA and then to sequence the amplicon. This study was carried out to screen the ability of thermophilic bacterial isolates for degrading protein by protease and to identify the most potent isolates using molecular techniques inferred by 16S rRNA gene. We are dependent on accurate sequences in databases, appropriate names associated with those sequences, and an accurate sequence for the isolate to be identified. Thirty seven bacterial isolates were cultured and characterized using nutrient casein plate method. Twenty out of thirty seven bacterial isolates have been recognized to be positive for casein test and selected for Gram staining method. Total DNA genomic were extracted by using I-genomic DNA Extraction Mini Kit, and polymerase chain reaction (PCR) operation assay was performed for appropriate target isolates. The phylogenetic analysis of these strains revealed that the genus of Bacillus is the most closely matched to the data from the GenBank. Bacillus subtilis, Bacillus sp, Bacillus amyloliquefaciens and Bacillus licheniformis were identified as species for the potential bacterial isolates. Two samples which are RSS6 and JF OUTER 2B have not been characterized through the phylogenetic tree. This might refer to either rare or new bacteria. ISSR 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/16419/1/final-essam.pdf Makky, Essam A. and Shaiful Zahid, M. Z. and Manaf, Almatar and M. M., Yusoff (2016) Preliminary Phylogenetic Analysis of Thermophilic Bacteria Producing Protease isolated from Kuantan Malaysia. Journal of Biotechnology Science Research (JBSR), 3 (5). pp. 166-174. ISSN 2409-9775 http://issres.net/jbsr/pubsystem/index.php/jbsr/article/view/30
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Makky, Essam A. Shaiful Zahid, M. Z. Manaf, Almatar M. M., Yusoff Preliminary Phylogenetic Analysis of Thermophilic Bacteria Producing Protease isolated from Kuantan Malaysia
description	Thermophilic bacteria grow in relatively high temperature between 50 to 60°C. These organisms can produce specific enzymes that have been used in industrial and biotechnological applications. The basic method to derive a sequence for a uncultured bacteria is to use universal primers against the 16S rRNA gene region in a PCR step to increase the amount of DNA and then to sequence the amplicon. This study was carried out to screen the ability of thermophilic bacterial isolates for degrading protein by protease and to identify the most potent isolates using molecular techniques inferred by 16S rRNA gene. We are dependent on accurate sequences in databases, appropriate names associated with those sequences, and an accurate sequence for the isolate to be identified. Thirty seven bacterial isolates were cultured and characterized using nutrient casein plate method. Twenty out of thirty seven bacterial isolates have been recognized to be positive for casein test and selected for Gram staining method. Total DNA genomic were extracted by using I-genomic DNA Extraction Mini Kit, and polymerase chain reaction (PCR) operation assay was performed for appropriate target isolates. The phylogenetic analysis of these strains revealed that the genus of Bacillus is the most closely matched to the data from the GenBank. Bacillus subtilis, Bacillus sp, Bacillus amyloliquefaciens and Bacillus licheniformis were identified as species for the potential bacterial isolates. Two samples which are RSS6 and JF OUTER 2B have not been characterized through the phylogenetic tree. This might refer to either rare or new bacteria.
format	Article
author	Makky, Essam A. Shaiful Zahid, M. Z. Manaf, Almatar M. M., Yusoff
author_facet	Makky, Essam A. Shaiful Zahid, M. Z. Manaf, Almatar M. M., Yusoff
author_sort	Makky, Essam A.
title	Preliminary Phylogenetic Analysis of Thermophilic Bacteria Producing Protease isolated from Kuantan Malaysia
title_short	Preliminary Phylogenetic Analysis of Thermophilic Bacteria Producing Protease isolated from Kuantan Malaysia
title_full	Preliminary Phylogenetic Analysis of Thermophilic Bacteria Producing Protease isolated from Kuantan Malaysia
title_fullStr	Preliminary Phylogenetic Analysis of Thermophilic Bacteria Producing Protease isolated from Kuantan Malaysia
title_full_unstemmed	Preliminary Phylogenetic Analysis of Thermophilic Bacteria Producing Protease isolated from Kuantan Malaysia
title_sort	preliminary phylogenetic analysis of thermophilic bacteria producing protease isolated from kuantan malaysia
publisher	ISSR
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/16419/ http://umpir.ump.edu.my/id/eprint/16419/ http://umpir.ump.edu.my/id/eprint/16419/1/final-essam.pdf
first_indexed	2023-09-18T22:22:04Z
last_indexed	2023-09-18T22:22:04Z
_version_	1777415723152310272

Preliminary Phylogenetic Analysis of Thermophilic Bacteria Producing Protease isolated from Kuantan Malaysia

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