Integration of Horseradish Peroxidase/Carbon Nanotube to Construct a Novel Platform for Direct Electrochemistry of Enxymes and Biosensing Applications

In this work, we describe a new 3-dimensional (3D) network of crosslinked Horseradish Peroxidase/Carbon Nanotube (HRP/CNT) on a thiol-modified Au surface in order to build up the effective electrical wiring of the enzyme units with the electrode. This is achieved by the electropolymerization of anil...

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Main Authors:	A. K. M., Kafi, Nina Suhaity, Azmi, M. M., Yusoff
Format:	Article
Language:	English
Published:	Pak Publishing Group 2016
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/16379/ http://umpir.ump.edu.my/id/eprint/16379/ http://umpir.ump.edu.my/id/eprint/16379/1/Integration%20of%20Horsedarsih.pdf

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recordtype	eprints
spelling	ump-163792018-01-24T03:47:11Z http://umpir.ump.edu.my/id/eprint/16379/ Integration of Horseradish Peroxidase/Carbon Nanotube to Construct a Novel Platform for Direct Electrochemistry of Enxymes and Biosensing Applications A. K. M., Kafi Nina Suhaity, Azmi M. M., Yusoff Q Science (General) In this work, we describe a new 3-dimensional (3D) network of crosslinked Horseradish Peroxidase/Carbon Nanotube (HRP/CNT) on a thiol-modified Au surface in order to build up the effective electrical wiring of the enzyme units with the electrode. This is achieved by the electropolymerization of aniline-functionalized carbon nanotubes (CNTs) and 4-aminothiophenol -modified-HRP on a 4-aminothiophenol monolayer-modified Au electrode. The synthesized 3D HRP/CNT network has been characterized with cyclic voltammetry and amperometry, resulting the establishment direct electron transfer between the redox active unit of HRP and the Au surface. Electrochemical measurements reveal that the immobilized HRP exhibits high biological activity and stability and a quasi-reversible redox peak of the redox centre of HRP was observed at about −0.355 and −0.275 V vs. Ag/AgCl. The electron transfer rate constant, KS and electron transfer co-efficient are found to be 0.57 s-1 and 0.42, respectively. Based on the electrocatalytic process by direct electrochemistry of HRP, a biosensor for detecting H2O2 is developed. The developed biosensor exhibits excellent electrocatalytic activity for the reduction of H2O2. The proposed biosensor modified with HRP/CNT 3D network displays a broader linear range and a lower detection limit for H2O2 determination. The linear range is from 1.0×10−7 to 1.2×10−4M with a detection limit of 2.2.0×10−8M at 3σ. The Michaelies–Menten constant Kapp M value is estimated to be 0.18 mM. Moreover, this biosensor exhibits very high sensitivity, good reproducibility and long-time stability. Ease of fabrication, a low cost, fast response and high sensitivity are the main advantages of the new biosensor proposed in this study. These obvious advantages would really help for the real analytical applicability of the proposed biosensor. Pak Publishing Group 2016-09-03 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/16379/1/Integration%20of%20Horsedarsih.pdf A. K. M., Kafi and Nina Suhaity, Azmi and M. M., Yusoff (2016) Integration of Horseradish Peroxidase/Carbon Nanotube to Construct a Novel Platform for Direct Electrochemistry of Enxymes and Biosensing Applications. Abstract of Applied Sciences and Engineering, 11. ISSN 2415-2072 http://www.pakinsight.com/pdf-files/pro/eng/20/ISCASE11-2016-15.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) A. K. M., Kafi Nina Suhaity, Azmi M. M., Yusoff Integration of Horseradish Peroxidase/Carbon Nanotube to Construct a Novel Platform for Direct Electrochemistry of Enxymes and Biosensing Applications
description	In this work, we describe a new 3-dimensional (3D) network of crosslinked Horseradish Peroxidase/Carbon Nanotube (HRP/CNT) on a thiol-modified Au surface in order to build up the effective electrical wiring of the enzyme units with the electrode. This is achieved by the electropolymerization of aniline-functionalized carbon nanotubes (CNTs) and 4-aminothiophenol -modified-HRP on a 4-aminothiophenol monolayer-modified Au electrode. The synthesized 3D HRP/CNT network has been characterized with cyclic voltammetry and amperometry, resulting the establishment direct electron transfer between the redox active unit of HRP and the Au surface. Electrochemical measurements reveal that the immobilized HRP exhibits high biological activity and stability and a quasi-reversible redox peak of the redox centre of HRP was observed at about −0.355 and −0.275 V vs. Ag/AgCl. The electron transfer rate constant, KS and electron transfer co-efficient are found to be 0.57 s-1 and 0.42, respectively. Based on the electrocatalytic process by direct electrochemistry of HRP, a biosensor for detecting H2O2 is developed. The developed biosensor exhibits excellent electrocatalytic activity for the reduction of H2O2. The proposed biosensor modified with HRP/CNT 3D network displays a broader linear range and a lower detection limit for H2O2 determination. The linear range is from 1.0×10−7 to 1.2×10−4M with a detection limit of 2.2.0×10−8M at 3σ. The Michaelies–Menten constant Kapp M value is estimated to be 0.18 mM. Moreover, this biosensor exhibits very high sensitivity, good reproducibility and long-time stability. Ease of fabrication, a low cost, fast response and high sensitivity are the main advantages of the new biosensor proposed in this study. These obvious advantages would really help for the real analytical applicability of the proposed biosensor.
format	Article
author	A. K. M., Kafi Nina Suhaity, Azmi M. M., Yusoff
author_facet	A. K. M., Kafi Nina Suhaity, Azmi M. M., Yusoff
author_sort	A. K. M., Kafi
title	Integration of Horseradish Peroxidase/Carbon Nanotube to Construct a Novel Platform for Direct Electrochemistry of Enxymes and Biosensing Applications
title_short	Integration of Horseradish Peroxidase/Carbon Nanotube to Construct a Novel Platform for Direct Electrochemistry of Enxymes and Biosensing Applications
title_full	Integration of Horseradish Peroxidase/Carbon Nanotube to Construct a Novel Platform for Direct Electrochemistry of Enxymes and Biosensing Applications
title_fullStr	Integration of Horseradish Peroxidase/Carbon Nanotube to Construct a Novel Platform for Direct Electrochemistry of Enxymes and Biosensing Applications
title_full_unstemmed	Integration of Horseradish Peroxidase/Carbon Nanotube to Construct a Novel Platform for Direct Electrochemistry of Enxymes and Biosensing Applications
title_sort	integration of horseradish peroxidase/carbon nanotube to construct a novel platform for direct electrochemistry of enxymes and biosensing applications
publisher	Pak Publishing Group
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/16379/ http://umpir.ump.edu.my/id/eprint/16379/ http://umpir.ump.edu.my/id/eprint/16379/1/Integration%20of%20Horsedarsih.pdf
first_indexed	2023-09-18T22:22:00Z
last_indexed	2023-09-18T22:22:00Z
_version_	1777415718254411776

Integration of Horseradish Peroxidase/Carbon Nanotube to Construct a Novel Platform for Direct Electrochemistry of Enxymes and Biosensing Applications

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