Development of a graphene based immuno-biosensor for hepatitis b virus surface antigen detection

Development of a graphene based immuno-biosensor for hepatitis b virus surface antigen detection

Hepatitis B virus (HBV) is a blood-borne and transfusion-transmitted human pathogen that has a large impact on blood safety and public health worldwide whereas reduced graphene oxide (RGO) is a derivatives of graphene which has gain much attention in electrochemical immunosensors. HBV infects the li...

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Bibliographic Details
Main Author: Geraldine Chan, Sue Ching
Format: Thesis
Language:English
English
Published: 2016
Subjects:
QD Chemistry
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/15784/
http://umpir.ump.edu.my/id/eprint/15784/
http://umpir.ump.edu.my/id/eprint/15784/1/Development%20of%20a%20graphene%20based%20immuno-biosensor%20for%20hepatitis%20b%20virus%20surface%20antigen%20detection%20-%20Table%20of%20contents%20-%20FKKSA-Geraldine%20Chan%20Sue%20Ching-CD9903.pdf
http://umpir.ump.edu.my/id/eprint/15784/2/Development%20of%20a%20graphene%20based%20immuno-biosensor%20for%20hepatitis%20b%20virus%20surface%20antigen%20detection%20-%20Abstract%20-%20FKKSA-Geraldine%20Chan%20Sue%20Ching-CD9903.pdf
id ump-15784
recordtype eprints
spelling ump-157842018-01-02T06:48:51Z http://umpir.ump.edu.my/id/eprint/15784/ Development of a graphene based immuno-biosensor for hepatitis b virus surface antigen detection Geraldine Chan, Sue Ching QD Chemistry TP Chemical technology Hepatitis B virus (HBV) is a blood-borne and transfusion-transmitted human pathogen that has a large impact on blood safety and public health worldwide whereas reduced graphene oxide (RGO) is a derivatives of graphene which has gain much attention in electrochemical immunosensors. HBV infects the liver and causes chronic and acute Hepatitis. The best treatment for this disease is for the early detection before the occurrence of severe liver damages. The presence of Hepatitis B surface antigen (HBsAg) is evidence of presence of HBV infections. Presently, the conventional methods shows weakness in terms of time and efficiency. In this research, it aims to immobilize the HBsAg antibody (HBsAb) onto functionalized RGO as well as to study the interaction between HBsAb and HBsAg by electrochemical probe of the functionalized RGO. RGO was synthesized via modified Hummer’s methods and reduced using hydrazine hydrate. RGO is functionalized with nafion and thionine prior to immobilization of HBsAb. Qualitative analysis of functionalized RGO was conducted via ultraviolet-visible spectrophotometry (UV-Vis), fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and field emission scanning electron microscopy (FESEM). Electrochemical analysis of functionalized RGO probe electrode on the detection of HBsAg was done via cyclic voltammetry (CV). As a conclusion, successful immobilization of HBsAg on functionalized RGO was proven to obey Freundlich adsorption isotherm with maximum adsorption capacity of 31 ng HBsAg/g and further confirmed with the change in surface structure observed as well as the presents of functional groups detected. Interaction between HBsAg and HBsAb causes the colour change when tetramethylbenzidine (TMB) was added and step changes to occur during CV with a limit of detection at 0.5 ng/mL. 2016-04 Thesis NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/15784/1/Development%20of%20a%20graphene%20based%20immuno-biosensor%20for%20hepatitis%20b%20virus%20surface%20antigen%20detection%20-%20Table%20of%20contents%20-%20FKKSA-Geraldine%20Chan%20Sue%20Ching-CD9903.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/15784/2/Development%20of%20a%20graphene%20based%20immuno-biosensor%20for%20hepatitis%20b%20virus%20surface%20antigen%20detection%20-%20Abstract%20-%20FKKSA-Geraldine%20Chan%20Sue%20Ching-CD9903.pdf Geraldine Chan, Sue Ching (2016) Development of a graphene based immuno-biosensor for hepatitis b virus surface antigen detection. Masters thesis, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:97795&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
English
topic QD Chemistry
TP Chemical technology
spellingShingle QD Chemistry
TP Chemical technology
Geraldine Chan, Sue Ching
Development of a graphene based immuno-biosensor for hepatitis b virus surface antigen detection
description Hepatitis B virus (HBV) is a blood-borne and transfusion-transmitted human pathogen that has a large impact on blood safety and public health worldwide whereas reduced graphene oxide (RGO) is a derivatives of graphene which has gain much attention in electrochemical immunosensors. HBV infects the liver and causes chronic and acute Hepatitis. The best treatment for this disease is for the early detection before the occurrence of severe liver damages. The presence of Hepatitis B surface antigen (HBsAg) is evidence of presence of HBV infections. Presently, the conventional methods shows weakness in terms of time and efficiency. In this research, it aims to immobilize the HBsAg antibody (HBsAb) onto functionalized RGO as well as to study the interaction between HBsAb and HBsAg by electrochemical probe of the functionalized RGO. RGO was synthesized via modified Hummer’s methods and reduced using hydrazine hydrate. RGO is functionalized with nafion and thionine prior to immobilization of HBsAb. Qualitative analysis of functionalized RGO was conducted via ultraviolet-visible spectrophotometry (UV-Vis), fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and field emission scanning electron microscopy (FESEM). Electrochemical analysis of functionalized RGO probe electrode on the detection of HBsAg was done via cyclic voltammetry (CV). As a conclusion, successful immobilization of HBsAg on functionalized RGO was proven to obey Freundlich adsorption isotherm with maximum adsorption capacity of 31 ng HBsAg/g and further confirmed with the change in surface structure observed as well as the presents of functional groups detected. Interaction between HBsAg and HBsAb causes the colour change when tetramethylbenzidine (TMB) was added and step changes to occur during CV with a limit of detection at 0.5 ng/mL.
format Thesis
author Geraldine Chan, Sue Ching
author_facet Geraldine Chan, Sue Ching
author_sort Geraldine Chan, Sue Ching
title Development of a graphene based immuno-biosensor for hepatitis b virus surface antigen detection
title_short Development of a graphene based immuno-biosensor for hepatitis b virus surface antigen detection
title_full Development of a graphene based immuno-biosensor for hepatitis b virus surface antigen detection
title_fullStr Development of a graphene based immuno-biosensor for hepatitis b virus surface antigen detection
title_full_unstemmed Development of a graphene based immuno-biosensor for hepatitis b virus surface antigen detection
title_sort development of a graphene based immuno-biosensor for hepatitis b virus surface antigen detection
publishDate 2016
url http://umpir.ump.edu.my/id/eprint/15784/
http://umpir.ump.edu.my/id/eprint/15784/
http://umpir.ump.edu.my/id/eprint/15784/1/Development%20of%20a%20graphene%20based%20immuno-biosensor%20for%20hepatitis%20b%20virus%20surface%20antigen%20detection%20-%20Table%20of%20contents%20-%20FKKSA-Geraldine%20Chan%20Sue%20Ching-CD9903.pdf
http://umpir.ump.edu.my/id/eprint/15784/2/Development%20of%20a%20graphene%20based%20immuno-biosensor%20for%20hepatitis%20b%20virus%20surface%20antigen%20detection%20-%20Abstract%20-%20FKKSA-Geraldine%20Chan%20Sue%20Ching-CD9903.pdf
first_indexed 2023-09-18T22:20:50Z
last_indexed 2023-09-18T22:20:50Z
_version_ 1777415644826828800

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