Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks
We report on a novel 3-dimensional (3D) network of crosslinked Cytochrome C/Carbon Nanotube (CytC/CNT) on a thiol-modified gold surface which can establish direct electrical communication between the redox center of Cytochrome C and the electrode. Cyclic voltammograms (CVs) results showed a pair of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Scientific Publishers
2017
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/15616/ http://umpir.ump.edu.my/id/eprint/15616/ http://umpir.ump.edu.my/id/eprint/15616/ http://umpir.ump.edu.my/id/eprint/15616/1/fist-2017-kafi-Superoxide%20Radical%20Biosensor%20Based1.pdf |
| Summary: | We report on a novel 3-dimensional (3D) network of crosslinked Cytochrome C/Carbon Nanotube (CytC/CNT) on a thiol-modified gold surface which can establish direct electrical communication between the redox center of Cytochrome C and the electrode. Cyclic voltammograms (CVs) results showed a pair of well defined redox peaks for Cytochrome C, located at about −0.03 and +0.06 V,
cathodic and anodic respectively. Additionally, the formal potential E0 of adsorbed Cyt c was found to be 15 mV, a value close to that of native Cyt c. Based on 3D Cytochrome c and carbon nanotube network, a sensitive superoxide radical biosensor has been proposed. The biosensor showed high sensitivity and lower detection limit of 0.3 �M of superoxide. |
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