Optimization of Succinic Acid from Fermentation Process by Using Immobiized Escherichia Coli Cells Via Anaerobic Fermentation

Optimization of Succinic Acid from Fermentation Process by Using Immobiized Escherichia Coli Cells Via Anaerobic Fermentation

Studies on succinic acid production by using immobilized cells are essential in the optimization and development of anaerobic fermentation process while glycerol treated used as substrate. One factor at one time (OFAT) method was performed as preliminary studies for selection of parameters range an...

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Main Authors: Nik Nor Aziati, Abdul Aziz, Mimi Sakinah, A. M.
Format: Conference or Workshop Item
Language:English
Published: Universiti Malaysia Pahang 2016
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/15548/
http://umpir.ump.edu.my/id/eprint/15548/
http://umpir.ump.edu.my/id/eprint/15548/1/P121%20pg908-912.pdf
id ump-15548
recordtype eprints
spelling ump-155482018-01-17T06:50:48Z http://umpir.ump.edu.my/id/eprint/15548/ Optimization of Succinic Acid from Fermentation Process by Using Immobiized Escherichia Coli Cells Via Anaerobic Fermentation Nik Nor Aziati, Abdul Aziz Mimi Sakinah, A. M. TP Chemical technology Studies on succinic acid production by using immobilized cells are essential in the optimization and development of anaerobic fermentation process while glycerol treated used as substrate. One factor at one time (OFAT) method was performed as preliminary studies for selection of parameters range and subsequently determine its optimum condition using response surface methodology (RSM). The optimum parameter combination was found at time 3.31 hour, 40 gram mass substrate (glycerol residue treated) and 15 % inoculums. Under these optimum conditions, 125.059 g/L succinic acid concentration was obtained. The succinic acid was found linearly dependent on time, mass substrates and inoculums. Multiple regression models with high R2 values were developed to correlate the succinic acid concentration as a function of time, mass substrate and inoculums. An analysis of variance (ANOVA) showed that the inoculums had slightly higher significant effect on succinic acid concentration if compared to time and mass substrates. Overall, this research showed that by using immobilized cells of Escherichia coli the production of succinic acid can be optimize and higher production of succinic acid. From this situation, the further study should be taken in genetic modification of Escherichia coli in immobilized cells to produce succinic acid as a major product to improve the fermentation process in producing the succinic acid for the future. Universiti Malaysia Pahang 2016 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/15548/1/P121%20pg908-912.pdf Nik Nor Aziati, Abdul Aziz and Mimi Sakinah, A. M. (2016) Optimization of Succinic Acid from Fermentation Process by Using Immobiized Escherichia Coli Cells Via Anaerobic Fermentation. In: Proceedings of The National Conference for Postgraduate Research (NCON-PGR 2016), 24-25 September 2016 , Universiti Malaysia Pahang (UMP), Pekan, Pahang. pp. 908-912.. http://ee.ump.edu.my/ncon/wp-content/uploads/2016/10/Proceeding-NCON-PGR-2016.zip
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Nik Nor Aziati, Abdul Aziz
Mimi Sakinah, A. M.
Optimization of Succinic Acid from Fermentation Process by Using Immobiized Escherichia Coli Cells Via Anaerobic Fermentation
description Studies on succinic acid production by using immobilized cells are essential in the optimization and development of anaerobic fermentation process while glycerol treated used as substrate. One factor at one time (OFAT) method was performed as preliminary studies for selection of parameters range and subsequently determine its optimum condition using response surface methodology (RSM). The optimum parameter combination was found at time 3.31 hour, 40 gram mass substrate (glycerol residue treated) and 15 % inoculums. Under these optimum conditions, 125.059 g/L succinic acid concentration was obtained. The succinic acid was found linearly dependent on time, mass substrates and inoculums. Multiple regression models with high R2 values were developed to correlate the succinic acid concentration as a function of time, mass substrate and inoculums. An analysis of variance (ANOVA) showed that the inoculums had slightly higher significant effect on succinic acid concentration if compared to time and mass substrates. Overall, this research showed that by using immobilized cells of Escherichia coli the production of succinic acid can be optimize and higher production of succinic acid. From this situation, the further study should be taken in genetic modification of Escherichia coli in immobilized cells to produce succinic acid as a major product to improve the fermentation process in producing the succinic acid for the future.
format Conference or Workshop Item
author Nik Nor Aziati, Abdul Aziz
Mimi Sakinah, A. M.
author_facet Nik Nor Aziati, Abdul Aziz
Mimi Sakinah, A. M.
author_sort Nik Nor Aziati, Abdul Aziz
title Optimization of Succinic Acid from Fermentation Process by Using Immobiized Escherichia Coli Cells Via Anaerobic Fermentation
title_short Optimization of Succinic Acid from Fermentation Process by Using Immobiized Escherichia Coli Cells Via Anaerobic Fermentation
title_full Optimization of Succinic Acid from Fermentation Process by Using Immobiized Escherichia Coli Cells Via Anaerobic Fermentation
title_fullStr Optimization of Succinic Acid from Fermentation Process by Using Immobiized Escherichia Coli Cells Via Anaerobic Fermentation
title_full_unstemmed Optimization of Succinic Acid from Fermentation Process by Using Immobiized Escherichia Coli Cells Via Anaerobic Fermentation
title_sort optimization of succinic acid from fermentation process by using immobiized escherichia coli cells via anaerobic fermentation
publisher Universiti Malaysia Pahang
publishDate 2016
url http://umpir.ump.edu.my/id/eprint/15548/
http://umpir.ump.edu.my/id/eprint/15548/
http://umpir.ump.edu.my/id/eprint/15548/1/P121%20pg908-912.pdf
first_indexed 2023-09-18T22:20:20Z
last_indexed 2023-09-18T22:20:20Z
_version_ 1777415613412540416

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