Comparative Analysis of Antioxidant and Antiproliferative Activities of Rhodomyrtus Tomentosa Extracts Prepared with Various Solvents

Rhodomyrtus tomentosa (Aiton) Hassk. has a wide spectrum of pharmacological effects and has been used to treat wounds, colic diarrhoea, heartburns, abscesses and gynaecopathy. The potential antiproliferative activities of R. tomentosa extracts from different solvents were evaluated in vitro on HepG2...

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Main Authors:	Hazrulrizawati, Hamid, Roziasyahira, Mutazah, M. M., Yusoff, Nurul Ashikin, Abd Karim, Ahmad Faizal, Abdull Razis
Format:	Article
Language:	English
Published:	Elsevier 2017
Subjects:	RM Therapeutics. Pharmacology
Online Access:	http://umpir.ump.edu.my/id/eprint/15476/ http://umpir.ump.edu.my/id/eprint/15476/ http://umpir.ump.edu.my/id/eprint/15476/ http://umpir.ump.edu.my/id/eprint/15476/1/fist-2017-hazrulrizawati-Comparative%20analysis1.pdf

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spelling	ump-154762018-07-26T07:47:08Z http://umpir.ump.edu.my/id/eprint/15476/ Comparative Analysis of Antioxidant and Antiproliferative Activities of Rhodomyrtus Tomentosa Extracts Prepared with Various Solvents Hazrulrizawati, Hamid Roziasyahira, Mutazah M. M., Yusoff Nurul Ashikin, Abd Karim Ahmad Faizal, Abdull Razis RM Therapeutics. Pharmacology Rhodomyrtus tomentosa (Aiton) Hassk. has a wide spectrum of pharmacological effects and has been used to treat wounds, colic diarrhoea, heartburns, abscesses and gynaecopathy. The potential antiproliferative activities of R. tomentosa extracts from different solvents were evaluated in vitro on HepG2, MCF-7 and HT 29 cell lines while antioxidant activity was monitored by radical scavenging assay (DPPH), copper reducing antioxidant capacity (CUPRAC) and b-carotene bleaching assay. Extracts from R. tomentosa show the viability of the cells in concentration-dependent manner. According to the IC50 obtained, the ethyl acetate extracts showed significant antiproliferative activity on HepG2 (IC50 11.47 ± 0.280 mg/mL), MCF-7(IC50 2.68 ± 0.529 mg/mL) and HT 29 (IC50 16.18 ± 0.538 mg/mL) after 72 h of treatment. Bioassay guided fractionation of the ethyl acetate extract led to the isolation of lupeol. Methanol extracts show significant antioxidant activities in DPPH (EC50 110.25 ± 0.005 mg/ml), CUPRAC (EC50 53.84 ± 0.004) and b-carotene bleaching (EC50 58.62 ± 0.001) due to the presence of high total flavonoid and total phenolic content which were 110.822 ± 0.017 mg butylated hydroxytoluene (BHT)/g and 190.467 ± 0.009 mg gallic acid (GAE)/g respectively. Taken together, the results extracts show the R. tomentosa as a potential source of antioxidant and antiproliferative efficacy. Elsevier 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/15476/1/fist-2017-hazrulrizawati-Comparative%20analysis1.pdf Hazrulrizawati, Hamid and Roziasyahira, Mutazah and M. M., Yusoff and Nurul Ashikin, Abd Karim and Ahmad Faizal, Abdull Razis (2017) Comparative Analysis of Antioxidant and Antiproliferative Activities of Rhodomyrtus Tomentosa Extracts Prepared with Various Solvents. Food and Chemical Toxicology, 108 (Pt. B). pp. 451-457. ISSN 0278-6915 https://doi.org/10.1016/j.fct.2016.10.004 doi: 10.1016/j.fct.2016.10.004
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	RM Therapeutics. Pharmacology
spellingShingle	RM Therapeutics. Pharmacology Hazrulrizawati, Hamid Roziasyahira, Mutazah M. M., Yusoff Nurul Ashikin, Abd Karim Ahmad Faizal, Abdull Razis Comparative Analysis of Antioxidant and Antiproliferative Activities of Rhodomyrtus Tomentosa Extracts Prepared with Various Solvents
description	Rhodomyrtus tomentosa (Aiton) Hassk. has a wide spectrum of pharmacological effects and has been used to treat wounds, colic diarrhoea, heartburns, abscesses and gynaecopathy. The potential antiproliferative activities of R. tomentosa extracts from different solvents were evaluated in vitro on HepG2, MCF-7 and HT 29 cell lines while antioxidant activity was monitored by radical scavenging assay (DPPH), copper reducing antioxidant capacity (CUPRAC) and b-carotene bleaching assay. Extracts from R. tomentosa show the viability of the cells in concentration-dependent manner. According to the IC50 obtained, the ethyl acetate extracts showed significant antiproliferative activity on HepG2 (IC50 11.47 ± 0.280 mg/mL), MCF-7(IC50 2.68 ± 0.529 mg/mL) and HT 29 (IC50 16.18 ± 0.538 mg/mL) after 72 h of treatment. Bioassay guided fractionation of the ethyl acetate extract led to the isolation of lupeol. Methanol extracts show significant antioxidant activities in DPPH (EC50 110.25 ± 0.005 mg/ml), CUPRAC (EC50 53.84 ± 0.004) and b-carotene bleaching (EC50 58.62 ± 0.001) due to the presence of high total flavonoid and total phenolic content which were 110.822 ± 0.017 mg butylated hydroxytoluene (BHT)/g and 190.467 ± 0.009 mg gallic acid (GAE)/g respectively. Taken together, the results extracts show the R. tomentosa as a potential source of antioxidant and antiproliferative efficacy.
format	Article
author	Hazrulrizawati, Hamid Roziasyahira, Mutazah M. M., Yusoff Nurul Ashikin, Abd Karim Ahmad Faizal, Abdull Razis
author_facet	Hazrulrizawati, Hamid Roziasyahira, Mutazah M. M., Yusoff Nurul Ashikin, Abd Karim Ahmad Faizal, Abdull Razis
author_sort	Hazrulrizawati, Hamid
title	Comparative Analysis of Antioxidant and Antiproliferative Activities of Rhodomyrtus Tomentosa Extracts Prepared with Various Solvents
title_short	Comparative Analysis of Antioxidant and Antiproliferative Activities of Rhodomyrtus Tomentosa Extracts Prepared with Various Solvents
title_full	Comparative Analysis of Antioxidant and Antiproliferative Activities of Rhodomyrtus Tomentosa Extracts Prepared with Various Solvents
title_fullStr	Comparative Analysis of Antioxidant and Antiproliferative Activities of Rhodomyrtus Tomentosa Extracts Prepared with Various Solvents
title_full_unstemmed	Comparative Analysis of Antioxidant and Antiproliferative Activities of Rhodomyrtus Tomentosa Extracts Prepared with Various Solvents
title_sort	comparative analysis of antioxidant and antiproliferative activities of rhodomyrtus tomentosa extracts prepared with various solvents
publisher	Elsevier
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/15476/ http://umpir.ump.edu.my/id/eprint/15476/ http://umpir.ump.edu.my/id/eprint/15476/ http://umpir.ump.edu.my/id/eprint/15476/1/fist-2017-hazrulrizawati-Comparative%20analysis1.pdf
first_indexed	2023-09-18T22:20:11Z
last_indexed	2023-09-18T22:20:11Z
_version_	1777415604179828736

Comparative Analysis of Antioxidant and Antiproliferative Activities of Rhodomyrtus Tomentosa Extracts Prepared with Various Solvents

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