Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads

Enzymes serving as biocatalysts and play an important roles in many industrial field. However, the limitation of enzyme usage due to its high cost and unstable conditions of soluble enzyme to harsh conditions lead to findings an alternative to enhance the enzyme efficiency by immobilization (insolub...

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Main Authors:	Mimi Sakinah, A. M., Siti Sabrina, M. S.
Format:	Conference or Workshop Item
Language:	English English
Published:	2016
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/14760/ http://umpir.ump.edu.my/id/eprint/14760/1/fkksa-2016-sabrina-Combination%20of%20Immobilization%20Techniques.pdf http://umpir.ump.edu.my/id/eprint/14760/7/fkksa-2016-sabrina-Combination%20of%20Immobilization%20Techniques1.pdf

id	ump-14760
recordtype	eprints
spelling	ump-147602018-01-17T06:30:02Z http://umpir.ump.edu.my/id/eprint/14760/ Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads Mimi Sakinah, A. M. Siti Sabrina, M. S. TP Chemical technology Enzymes serving as biocatalysts and play an important roles in many industrial field. However, the limitation of enzyme usage due to its high cost and unstable conditions of soluble enzyme to harsh conditions lead to findings an alternative to enhance the enzyme efficiency by immobilization (insoluble enzyme). The present work reported a combination of immobilization technique of xylanase by entrapment and covalent binding on alginate hydrogel beads. Xylanase enzyme was effectively immobilized within the alginate hydrogel beads by entrapment and covalent binding on the surface of alginate beads using glutaraldehyde as a cross-linked agent. The effects of immobilization parameters include of sodium alginate concentration (% w/v), calcium chloride (M), and glutaraldehyde concentration (% w/v) were studied in order to obtain a better immobilization yield. These effects were studied using one-factor-at-one-time (OFAT) to obtain the best conditions for xylanase immobilization. The analysis of xylanase activity was determined using dinitrosalicyclic (DNS) acid reagent method. Maximal enzyme immobilization yield (>80 %) was achieved at 3.0 % w/v sodium alginate concentration, 0.3 M calcium alginate, and 9 % w/v of glutaraldehyde concentration. The study shows the xylanase can be immobilized efficiently by a combination of immobilization techniques by entrapment and covalent binding on alginate hydrogel beads 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/14760/1/fkksa-2016-sabrina-Combination%20of%20Immobilization%20Techniques.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/14760/7/fkksa-2016-sabrina-Combination%20of%20Immobilization%20Techniques1.pdf Mimi Sakinah, A. M. and Siti Sabrina, M. S. (2016) Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads. In: International Conference in Organic Synthesis 2016 (ICOS2016), 21-24 August 2016 , Kuching, Sarawak. pp. 1-10..
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Mimi Sakinah, A. M. Siti Sabrina, M. S. Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads
description	Enzymes serving as biocatalysts and play an important roles in many industrial field. However, the limitation of enzyme usage due to its high cost and unstable conditions of soluble enzyme to harsh conditions lead to findings an alternative to enhance the enzyme efficiency by immobilization (insoluble enzyme). The present work reported a combination of immobilization technique of xylanase by entrapment and covalent binding on alginate hydrogel beads. Xylanase enzyme was effectively immobilized within the alginate hydrogel beads by entrapment and covalent binding on the surface of alginate beads using glutaraldehyde as a cross-linked agent. The effects of immobilization parameters include of sodium alginate concentration (% w/v), calcium chloride (M), and glutaraldehyde concentration (% w/v) were studied in order to obtain a better immobilization yield. These effects were studied using one-factor-at-one-time (OFAT) to obtain the best conditions for xylanase immobilization. The analysis of xylanase activity was determined using dinitrosalicyclic (DNS) acid reagent method. Maximal enzyme immobilization yield (>80 %) was achieved at 3.0 % w/v sodium alginate concentration, 0.3 M calcium alginate, and 9 % w/v of glutaraldehyde concentration. The study shows the xylanase can be immobilized efficiently by a combination of immobilization techniques by entrapment and covalent binding on alginate hydrogel beads
format	Conference or Workshop Item
author	Mimi Sakinah, A. M. Siti Sabrina, M. S.
author_facet	Mimi Sakinah, A. M. Siti Sabrina, M. S.
author_sort	Mimi Sakinah, A. M.
title	Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads
title_short	Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads
title_full	Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads
title_fullStr	Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads
title_full_unstemmed	Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads
title_sort	combination of immobilization techniques of xylanase by entrapment and covalent binding on alginate hydrogel beads
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/14760/ http://umpir.ump.edu.my/id/eprint/14760/1/fkksa-2016-sabrina-Combination%20of%20Immobilization%20Techniques.pdf http://umpir.ump.edu.my/id/eprint/14760/7/fkksa-2016-sabrina-Combination%20of%20Immobilization%20Techniques1.pdf
first_indexed	2023-09-18T22:18:52Z
last_indexed	2023-09-18T22:18:52Z
_version_	1777415521370636288

Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads

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