Optimization of the Enzymatic Saccharification Process of Empty Fruit Bunch Pretreated with Laccase Enzyme

The saccharification of laccase-pretreated empty fruit bunch (EFB) was optimized in a lab-scale experiment using one-factor-at-a-time (OFAT) and response surface methodology (RSM). After pretreatment, the degree of delignification was checked by noting the weight loss (%) after pretreatment, and als...

Full description

Bibliographic Details
Main Authors:	Rashid, Shah Samiur, Ishmael, Ukaegbu Chinonso, Jaya Vejayan, Palliah, Mohammed Fazli, Farida Asras, Sharifah Suhaiza, Nik Wan Ahmad
Format:	Article
Language:	English English
Published:	North Carolina State University 2016
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/14679/ http://umpir.ump.edu.my/id/eprint/14679/ http://umpir.ump.edu.my/id/eprint/14679/1/Optimization%20of%20the%20Enzymatic%20Saccharification%20Process%20of%20Empty%20Fruit%20Bunch%20Pretreated%20with%20Laccase%20Enzyme.pdf http://umpir.ump.edu.my/id/eprint/14679/6/fist-2016-jaya-Optimization%20of%20the%20Enzymatic%20Saccharification.pdf

id	ump-14679
recordtype	eprints
spelling	ump-146792020-02-21T07:36:05Z http://umpir.ump.edu.my/id/eprint/14679/ Optimization of the Enzymatic Saccharification Process of Empty Fruit Bunch Pretreated with Laccase Enzyme Rashid, Shah Samiur Ishmael, Ukaegbu Chinonso Jaya Vejayan, Palliah Mohammed Fazli, Farida Asras Sharifah Suhaiza, Nik Wan Ahmad Q Science (General) The saccharification of laccase-pretreated empty fruit bunch (EFB) was optimized in a lab-scale experiment using one-factor-at-a-time (OFAT) and response surface methodology (RSM). After pretreatment, the degree of delignification was checked by noting the weight loss (%) after pretreatment, and also by the quantity of total sugar produced after saccharification with cellulase enzyme. OFAT studies of saccharification of the pretreated EFB showed that the biomass was best saccharified using cellulase enzyme at the following conditions: enzyme concentration of 30 IU/g of EFB, substrate concentration of 5.0% w/v, 50 °C, saccharification time of 24 h, and pH 5. This combination exhibited the highest yield of total sugar (28% w/w). Although 29% w/w yield was achieved with an enzyme concentration of 40 IU/g of EFB, this increase in yield was not proportional to the increased enzyme concentration and, therefore, was considered insignificant. Statistical analysis of the combined effects of pH and temperature showed that pH had a more significant effect than the temperature on the saccharification process, based on a P < 0.05 significance level. The effect of pH on total sugar production was more significant than the temperature in both linear and quadratic functions. In sum, the saccharification of laccase-pretreated EFB should follow the optimized process conditions achieved in the current study. North Carolina State University 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/14679/1/Optimization%20of%20the%20Enzymatic%20Saccharification%20Process%20of%20Empty%20Fruit%20Bunch%20Pretreated%20with%20Laccase%20Enzyme.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/14679/6/fist-2016-jaya-Optimization%20of%20the%20Enzymatic%20Saccharification.pdf Rashid, Shah Samiur and Ishmael, Ukaegbu Chinonso and Jaya Vejayan, Palliah and Mohammed Fazli, Farida Asras and Sharifah Suhaiza, Nik Wan Ahmad (2016) Optimization of the Enzymatic Saccharification Process of Empty Fruit Bunch Pretreated with Laccase Enzyme. BioResources, 11 (2). pp. 5138-5154. ISSN 1930-2126 (print); 1930-2126 (online) http://152.1.0.246/index.php/BioRes/article/view/BioRes_11_2_5138_Shah_Optimization_Enzymatic_Saccharification
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Rashid, Shah Samiur Ishmael, Ukaegbu Chinonso Jaya Vejayan, Palliah Mohammed Fazli, Farida Asras Sharifah Suhaiza, Nik Wan Ahmad Optimization of the Enzymatic Saccharification Process of Empty Fruit Bunch Pretreated with Laccase Enzyme
description	The saccharification of laccase-pretreated empty fruit bunch (EFB) was optimized in a lab-scale experiment using one-factor-at-a-time (OFAT) and response surface methodology (RSM). After pretreatment, the degree of delignification was checked by noting the weight loss (%) after pretreatment, and also by the quantity of total sugar produced after saccharification with cellulase enzyme. OFAT studies of saccharification of the pretreated EFB showed that the biomass was best saccharified using cellulase enzyme at the following conditions: enzyme concentration of 30 IU/g of EFB, substrate concentration of 5.0% w/v, 50 °C, saccharification time of 24 h, and pH 5. This combination exhibited the highest yield of total sugar (28% w/w). Although 29% w/w yield was achieved with an enzyme concentration of 40 IU/g of EFB, this increase in yield was not proportional to the increased enzyme concentration and, therefore, was considered insignificant. Statistical analysis of the combined effects of pH and temperature showed that pH had a more significant effect than the temperature on the saccharification process, based on a P < 0.05 significance level. The effect of pH on total sugar production was more significant than the temperature in both linear and quadratic functions. In sum, the saccharification of laccase-pretreated EFB should follow the optimized process conditions achieved in the current study.
format	Article
author	Rashid, Shah Samiur Ishmael, Ukaegbu Chinonso Jaya Vejayan, Palliah Mohammed Fazli, Farida Asras Sharifah Suhaiza, Nik Wan Ahmad
author_facet	Rashid, Shah Samiur Ishmael, Ukaegbu Chinonso Jaya Vejayan, Palliah Mohammed Fazli, Farida Asras Sharifah Suhaiza, Nik Wan Ahmad
author_sort	Rashid, Shah Samiur
title	Optimization of the Enzymatic Saccharification Process of Empty Fruit Bunch Pretreated with Laccase Enzyme
title_short	Optimization of the Enzymatic Saccharification Process of Empty Fruit Bunch Pretreated with Laccase Enzyme
title_full	Optimization of the Enzymatic Saccharification Process of Empty Fruit Bunch Pretreated with Laccase Enzyme
title_fullStr	Optimization of the Enzymatic Saccharification Process of Empty Fruit Bunch Pretreated with Laccase Enzyme
title_full_unstemmed	Optimization of the Enzymatic Saccharification Process of Empty Fruit Bunch Pretreated with Laccase Enzyme
title_sort	optimization of the enzymatic saccharification process of empty fruit bunch pretreated with laccase enzyme
publisher	North Carolina State University
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/14679/ http://umpir.ump.edu.my/id/eprint/14679/ http://umpir.ump.edu.my/id/eprint/14679/1/Optimization%20of%20the%20Enzymatic%20Saccharification%20Process%20of%20Empty%20Fruit%20Bunch%20Pretreated%20with%20Laccase%20Enzyme.pdf http://umpir.ump.edu.my/id/eprint/14679/6/fist-2016-jaya-Optimization%20of%20the%20Enzymatic%20Saccharification.pdf
first_indexed	2023-09-18T22:18:41Z
last_indexed	2023-09-18T22:18:41Z
_version_	1777415509874049024

Optimization of the Enzymatic Saccharification Process of Empty Fruit Bunch Pretreated with Laccase Enzyme

Similar Items