Determination of Green Fluorescent Protein by Ultraviolet-Excited Gel Imaging

The green fluorescent protein has gained interest in bioanalytical applications due to its visible fluorescence. As the usage of green fluorescent protein increases, more appropriate fluorescence instrumentation is required. Most fluorescence instrumentation uses ultraviolet light as the excitation...

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Main Authors:	Hor, Chee Heng, Chew, Few Ne
Format:	Article
Language:	English
Published:	Taylor & Francis 2016
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/14577/ http://umpir.ump.edu.my/id/eprint/14577/ http://umpir.ump.edu.my/id/eprint/14577/ http://umpir.ump.edu.my/id/eprint/14577/7/fkksa-2016-chew-Determination%20Of%20Green%20Fluorescent%20Protein.pdf

id	ump-14577
recordtype	eprints
spelling	ump-145772017-12-15T06:44:55Z http://umpir.ump.edu.my/id/eprint/14577/ Determination of Green Fluorescent Protein by Ultraviolet-Excited Gel Imaging Hor, Chee Heng Chew, Few Ne TP Chemical technology The green fluorescent protein has gained interest in bioanalytical applications due to its visible fluorescence. As the usage of green fluorescent protein increases, more appropriate fluorescence instrumentation is required. Most fluorescence instrumentation uses ultraviolet light as the excitation source for the determination of green fluorescent protein. However, ultraviolet radiation may damage biological molecules and affect the quantitative analysis. In this study, the effects of the ultraviolet radiation period and the mass of green fluorescent protein on the fluorescence determination were characterized using gel imaging. The ultraviolet illumination period affected the green fluorescent protein fluorescence intensity. The fluorescence increased with the ultraviolet illumination time from 30–90 s. However, the fluorescence intensity decreased when the excitation period was longer, probably due to photobleaching. The photobleaching decreased when a higher concentration of enhanced green fluorescent protein was employed. This gel imaging study has provided a better understanding of the optimum conditions for the determination of green fluorescent protein. Taylor & Francis 2016-04-11 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/14577/7/fkksa-2016-chew-Determination%20Of%20Green%20Fluorescent%20Protein.pdf Hor, Chee Heng and Chew, Few Ne (2016) Determination of Green Fluorescent Protein by Ultraviolet-Excited Gel Imaging. Instrumentation Science and Technology, 44 (5). pp. 558-565. ISSN 1525-6030 http://dx.doi.org/10.1080/10739149.2016.1170032 DOI: 10.1080/10739149.2016.1170032
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Hor, Chee Heng Chew, Few Ne Determination of Green Fluorescent Protein by Ultraviolet-Excited Gel Imaging
description	The green fluorescent protein has gained interest in bioanalytical applications due to its visible fluorescence. As the usage of green fluorescent protein increases, more appropriate fluorescence instrumentation is required. Most fluorescence instrumentation uses ultraviolet light as the excitation source for the determination of green fluorescent protein. However, ultraviolet radiation may damage biological molecules and affect the quantitative analysis. In this study, the effects of the ultraviolet radiation period and the mass of green fluorescent protein on the fluorescence determination were characterized using gel imaging. The ultraviolet illumination period affected the green fluorescent protein fluorescence intensity. The fluorescence increased with the ultraviolet illumination time from 30–90 s. However, the fluorescence intensity decreased when the excitation period was longer, probably due to photobleaching. The photobleaching decreased when a higher concentration of enhanced green fluorescent protein was employed. This gel imaging study has provided a better understanding of the optimum conditions for the determination of green fluorescent protein.
format	Article
author	Hor, Chee Heng Chew, Few Ne
author_facet	Hor, Chee Heng Chew, Few Ne
author_sort	Hor, Chee Heng
title	Determination of Green Fluorescent Protein by Ultraviolet-Excited Gel Imaging
title_short	Determination of Green Fluorescent Protein by Ultraviolet-Excited Gel Imaging
title_full	Determination of Green Fluorescent Protein by Ultraviolet-Excited Gel Imaging
title_fullStr	Determination of Green Fluorescent Protein by Ultraviolet-Excited Gel Imaging
title_full_unstemmed	Determination of Green Fluorescent Protein by Ultraviolet-Excited Gel Imaging
title_sort	determination of green fluorescent protein by ultraviolet-excited gel imaging
publisher	Taylor & Francis
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/14577/ http://umpir.ump.edu.my/id/eprint/14577/ http://umpir.ump.edu.my/id/eprint/14577/ http://umpir.ump.edu.my/id/eprint/14577/7/fkksa-2016-chew-Determination%20Of%20Green%20Fluorescent%20Protein.pdf
first_indexed	2023-09-18T22:18:29Z
last_indexed	2023-09-18T22:18:29Z
_version_	1777415497934962688

Determination of Green Fluorescent Protein by Ultraviolet-Excited Gel Imaging

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