Cytotoxicity of Clinacanthus Nutans Extracts on Human Hepatoma (HepG2) Cell Line

Objective: Clinacanthus nutans is one of the herbs that has been used in Asia as a traditional medicine for the treatment of serious diseases. The aim of this study is to investigate the phytochemical constituents (flavonoids and phenolics) and cytotoxicity against human hepatoma (HepG2) cancer ce...

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Main Authors:	Hazrulrizawati, Hamid, Izzah Hayati, Yahaya
Format:	Article
Language:	English
Published:	Innovare Academic Sciences 2016
Subjects:	RM Therapeutics. Pharmacology
Online Access:	http://umpir.ump.edu.my/id/eprint/14494/ http://umpir.ump.edu.my/id/eprint/14494/ http://umpir.ump.edu.my/id/eprint/14494/ http://umpir.ump.edu.my/id/eprint/14494/1/13626-55421-1-CE-1-izzah.pdf

id	ump-14494
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spelling	ump-144942017-01-10T06:48:32Z http://umpir.ump.edu.my/id/eprint/14494/ Cytotoxicity of Clinacanthus Nutans Extracts on Human Hepatoma (HepG2) Cell Line Hazrulrizawati, Hamid Izzah Hayati, Yahaya RM Therapeutics. Pharmacology Objective: Clinacanthus nutans is one of the herbs that has been used in Asia as a traditional medicine for the treatment of serious diseases. The aim of this study is to investigate the phytochemical constituents (flavonoids and phenolics) and cytotoxicity against human hepatoma (HepG2) cancer cell lines of C. nutans extracts. Methods: The fractions from C. nutans were extracted from hexane, methanol, chloroform and ethyl acetate by the solvent-solvent extraction method. The crude extracts (10 mg/ml) were tested against HepG2 cell lines using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Phytochemical screening was done to determine the total phenolic content (TPC) and total flavonoid content (TFC). Results: Methanol extract showed the strongest cytotoxic activity against HepG2 cell line with IC 50 of 43.9367μg/ml after 24 h of treatment compared to chloroform extract and ethyl acetate, 55.6112µg/ml and 62.0655µg/ml, respectively. Hexane extract formed the lowest cytotoxicity activity with IC50 of 68.3807µg/ml. Total phenolic content (TPC) was found to be highest in chloroform, which was 119.29 mg of gallic acid equivalent (GAE) and total flavonoid content (TFC), methanol was performed the highest value, which is 937.67 mg of butylated hydroxytoluene (BHT). Conclusion: Different active compounds present in the extracts may contribute different cytotoxicity effects of crude extracts. The relationship data of total phenolic, total flavonoid, and cytotoxic potential of C. nutans, indicates that these plants might contain valuable active compounds as a chemotherapeutic agent. Further investigations to elucidate the chemical structures of active compounds are necessary for potential compounds discovery in drugs. Innovare Academic Sciences 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/14494/1/13626-55421-1-CE-1-izzah.pdf Hazrulrizawati, Hamid and Izzah Hayati, Yahaya (2016) Cytotoxicity of Clinacanthus Nutans Extracts on Human Hepatoma (HepG2) Cell Line. International Journal of Pharmacy and Pharmaceutical Sciences, 8 (10). pp. 293-295. ISSN 0975–1491 http://dx.doi.org/10.22159/ijpps.2016v8i10.13626 dx.doi.org/10.22159/ijpps.2016v8i10.13626
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	RM Therapeutics. Pharmacology
spellingShingle	RM Therapeutics. Pharmacology Hazrulrizawati, Hamid Izzah Hayati, Yahaya Cytotoxicity of Clinacanthus Nutans Extracts on Human Hepatoma (HepG2) Cell Line
description	Objective: Clinacanthus nutans is one of the herbs that has been used in Asia as a traditional medicine for the treatment of serious diseases. The aim of this study is to investigate the phytochemical constituents (flavonoids and phenolics) and cytotoxicity against human hepatoma (HepG2) cancer cell lines of C. nutans extracts. Methods: The fractions from C. nutans were extracted from hexane, methanol, chloroform and ethyl acetate by the solvent-solvent extraction method. The crude extracts (10 mg/ml) were tested against HepG2 cell lines using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Phytochemical screening was done to determine the total phenolic content (TPC) and total flavonoid content (TFC). Results: Methanol extract showed the strongest cytotoxic activity against HepG2 cell line with IC 50 of 43.9367μg/ml after 24 h of treatment compared to chloroform extract and ethyl acetate, 55.6112µg/ml and 62.0655µg/ml, respectively. Hexane extract formed the lowest cytotoxicity activity with IC50 of 68.3807µg/ml. Total phenolic content (TPC) was found to be highest in chloroform, which was 119.29 mg of gallic acid equivalent (GAE) and total flavonoid content (TFC), methanol was performed the highest value, which is 937.67 mg of butylated hydroxytoluene (BHT). Conclusion: Different active compounds present in the extracts may contribute different cytotoxicity effects of crude extracts. The relationship data of total phenolic, total flavonoid, and cytotoxic potential of C. nutans, indicates that these plants might contain valuable active compounds as a chemotherapeutic agent. Further investigations to elucidate the chemical structures of active compounds are necessary for potential compounds discovery in drugs.
format	Article
author	Hazrulrizawati, Hamid Izzah Hayati, Yahaya
author_facet	Hazrulrizawati, Hamid Izzah Hayati, Yahaya
author_sort	Hazrulrizawati, Hamid
title	Cytotoxicity of Clinacanthus Nutans Extracts on Human Hepatoma (HepG2) Cell Line
title_short	Cytotoxicity of Clinacanthus Nutans Extracts on Human Hepatoma (HepG2) Cell Line
title_full	Cytotoxicity of Clinacanthus Nutans Extracts on Human Hepatoma (HepG2) Cell Line
title_fullStr	Cytotoxicity of Clinacanthus Nutans Extracts on Human Hepatoma (HepG2) Cell Line
title_full_unstemmed	Cytotoxicity of Clinacanthus Nutans Extracts on Human Hepatoma (HepG2) Cell Line
title_sort	cytotoxicity of clinacanthus nutans extracts on human hepatoma (hepg2) cell line
publisher	Innovare Academic Sciences
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/14494/ http://umpir.ump.edu.my/id/eprint/14494/ http://umpir.ump.edu.my/id/eprint/14494/ http://umpir.ump.edu.my/id/eprint/14494/1/13626-55421-1-CE-1-izzah.pdf
first_indexed	2023-09-18T22:18:18Z
last_indexed	2023-09-18T22:18:18Z
_version_	1777415486098636800

Cytotoxicity of Clinacanthus Nutans Extracts on Human Hepatoma (HepG2) Cell Line

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