Bioremediation of Disposed X-Ray Film For Enzymes Production

Annually, the production of X-ray films sheets can utilize up to 1000 tons of total silver chemically produced worldwide and being wasted when these films are used. To avoid waste, the biological methods are used to study the effect of disposed X-ray on production of two different types of enzyme...

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Main Authors:	Noor Afifah, Fauzi, Siti Hajar, Mohd Rasdi, Makky, Essam A., Mohd Hasbi, Ab. Rahim
Format:	Article
Language:	English English
Published:	Scholarly Peer Review Publishing System 2016
Subjects:	QR Microbiology
Online Access:	http://umpir.ump.edu.my/id/eprint/13545/ http://umpir.ump.edu.my/id/eprint/13545/ http://umpir.ump.edu.my/id/eprint/13545/1/GJAR_2016.pdf http://umpir.ump.edu.my/id/eprint/13545/7/fist-2016-essam-Bioremediation%20of%20Disposed%20X-Ray.pdf

id	ump-13545
recordtype	eprints
spelling	ump-135452018-11-22T07:18:12Z http://umpir.ump.edu.my/id/eprint/13545/ Bioremediation of Disposed X-Ray Film For Enzymes Production Noor Afifah, Fauzi Siti Hajar, Mohd Rasdi Makky, Essam A. Mohd Hasbi, Ab. Rahim QR Microbiology Annually, the production of X-ray films sheets can utilize up to 1000 tons of total silver chemically produced worldwide and being wasted when these films are used. To avoid waste, the biological methods are used to study the effect of disposed X-ray on production of two different types of enzyme by using microbial power. Firstly, production of CMCase enzyme by using disposed X-ray as a carbon source. Secondly, production of nitrate reductase enzyme responsible to catalyse the reduction of silver nitrate inside X-ray film. Both bring the disposed X-ray as a substrate. Different bacterial isolates were used for production of both enzymes and being optimized by using several parameters. The protein and enzyme assay were analysed using optical density measurement. CMCase production found to be optimal in 140 rpm incubator with lactose as carbon source by 1.365±0.026 (U/ml), malt extract as nitrogen source by 0.485±0.028 (U/ml), pH 9.0 by 0.129±0.007 (U/ml), 1.5 g substrate concentration by 0.217±0.007 (U/ml), 2ml inoculum size by 0.143±0.029 (U/ml), and thiamine for vitamin by 0.208±0.041 (U/ml). While nitrate reductase production, the most potent isolates achieved optimum condition in static incubation condition by 1.182±0.000μg/ml, 0.5g substrate concentration by 1.500±0.020μg/ml with 15 days incubation periods in darkness, NaNO3 as nitrogen source by 166.773±0.054μg/ml and pH 3.0 by 0.773±0.001μg/ml. This study proved that the power of most potent isolates successfully used the disposed X-ray as substrate to produce valuable by-products using green technology to reduce environmental pollution. Scholarly Peer Review Publishing System 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/13545/1/GJAR_2016.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/13545/7/fist-2016-essam-Bioremediation%20of%20Disposed%20X-Ray.pdf Noor Afifah, Fauzi and Siti Hajar, Mohd Rasdi and Makky, Essam A. and Mohd Hasbi, Ab. Rahim (2016) Bioremediation of Disposed X-Ray Film For Enzymes Production. Global Journal of Advanced Research, 3 (2). pp. 101-106. ISSN 2394-5788 http://www.gjar.org/publishpaper/vol3issue2/d425r39.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	QR Microbiology
spellingShingle	QR Microbiology Noor Afifah, Fauzi Siti Hajar, Mohd Rasdi Makky, Essam A. Mohd Hasbi, Ab. Rahim Bioremediation of Disposed X-Ray Film For Enzymes Production
description	Annually, the production of X-ray films sheets can utilize up to 1000 tons of total silver chemically produced worldwide and being wasted when these films are used. To avoid waste, the biological methods are used to study the effect of disposed X-ray on production of two different types of enzyme by using microbial power. Firstly, production of CMCase enzyme by using disposed X-ray as a carbon source. Secondly, production of nitrate reductase enzyme responsible to catalyse the reduction of silver nitrate inside X-ray film. Both bring the disposed X-ray as a substrate. Different bacterial isolates were used for production of both enzymes and being optimized by using several parameters. The protein and enzyme assay were analysed using optical density measurement. CMCase production found to be optimal in 140 rpm incubator with lactose as carbon source by 1.365±0.026 (U/ml), malt extract as nitrogen source by 0.485±0.028 (U/ml), pH 9.0 by 0.129±0.007 (U/ml), 1.5 g substrate concentration by 0.217±0.007 (U/ml), 2ml inoculum size by 0.143±0.029 (U/ml), and thiamine for vitamin by 0.208±0.041 (U/ml). While nitrate reductase production, the most potent isolates achieved optimum condition in static incubation condition by 1.182±0.000μg/ml, 0.5g substrate concentration by 1.500±0.020μg/ml with 15 days incubation periods in darkness, NaNO3 as nitrogen source by 166.773±0.054μg/ml and pH 3.0 by 0.773±0.001μg/ml. This study proved that the power of most potent isolates successfully used the disposed X-ray as substrate to produce valuable by-products using green technology to reduce environmental pollution.
format	Article
author	Noor Afifah, Fauzi Siti Hajar, Mohd Rasdi Makky, Essam A. Mohd Hasbi, Ab. Rahim
author_facet	Noor Afifah, Fauzi Siti Hajar, Mohd Rasdi Makky, Essam A. Mohd Hasbi, Ab. Rahim
author_sort	Noor Afifah, Fauzi
title	Bioremediation of Disposed X-Ray Film For Enzymes Production
title_short	Bioremediation of Disposed X-Ray Film For Enzymes Production
title_full	Bioremediation of Disposed X-Ray Film For Enzymes Production
title_fullStr	Bioremediation of Disposed X-Ray Film For Enzymes Production
title_full_unstemmed	Bioremediation of Disposed X-Ray Film For Enzymes Production
title_sort	bioremediation of disposed x-ray film for enzymes production
publisher	Scholarly Peer Review Publishing System
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/13545/ http://umpir.ump.edu.my/id/eprint/13545/ http://umpir.ump.edu.my/id/eprint/13545/1/GJAR_2016.pdf http://umpir.ump.edu.my/id/eprint/13545/7/fist-2016-essam-Bioremediation%20of%20Disposed%20X-Ray.pdf
first_indexed	2023-09-18T22:16:18Z
last_indexed	2023-09-18T22:16:18Z
_version_	1777415359741034496

Bioremediation of Disposed X-Ray Film For Enzymes Production

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