Microbiological Study against Bacterial Isolates from Different Patients
Tooth decay is considered the most widespread infectious disease in the world. This study aims to isolate and identify the important bacteria related to tooth decay, determine the sensitivity of bacteria in certain types of antimicrobial agents, and study the effect of heavy metals and virulence fa...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
AENSI Publishing
2016
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/13544/ http://umpir.ump.edu.my/id/eprint/13544/ http://umpir.ump.edu.my/id/eprint/13544/1/fist-2016-essam-microbiology.pdf |
| Summary: | Tooth decay is considered the most widespread infectious disease in the world. This study aims to isolate and identify the important bacteria related to tooth decay,
determine the sensitivity of bacteria in certain types of antimicrobial agents, and study the effect of heavy metals and virulence factors on oral bacterial isolates. A total of 50 swabs were collected from the mouths of patients from both gender, with ages ranging from 1–60 years. The patients were advised to consult with dental clinics and
specialized centers to isolate and identify the causative agents associated with oral diseases. Results showed that infection rates in younger age groups (1–20 and 20–40)
are higher than the elder group (40–60), with percent incidence of 44% and 32%, respectively. Antibiotic sensitivity test against the isolates showed that
chloramphenicol had the highest sensitivity effect with 83.2% followed by rifampicin and gentamicin with 81.35%, penicillin G with 64.40%, and streptomycin with 16.94%
Also, these differences were found have lower effect for isolates against (10) heavy metals, where it showed resistance to Iron 3.38%, then nickel, aluminum, copper, lead to 20.33%, 22.03%, 27.11%, 28.81% respectively, also sliver shown 57.62%. And, this similarity were found have sensitive to antimony and chromium 61.01%.while appeared sensitivity to mercury and cadmium by 100, 86.44. Hemolysin had the highest ability to produce virulence factors (72.88%), followed by lecithinase (42.37%) and protease (25.42%). Lipase and urease had the lowest virulence factor production (10.16%). |
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