Optimisation of phenolic compounds extraction from misai kucing using RSM

Misai kucing or the scientific name is Orthosiphon stamineus contains lots of phenolic compounds such as phenolic acid, flavanoids and antioxidants. These compounds have various benefits likes antifungal, antimicrobial, antitumor and antibacterial. Besides that, people also used it as remedy to trea...

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Main Author:	Ainin Sofia, Azni
Format:	Undergraduates Project Papers
Language:	English English English
Published:	2015
Subjects:	Q Science (General) TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/12750/ http://umpir.ump.edu.my/id/eprint/12750/ http://umpir.ump.edu.my/id/eprint/12750/1/Optimisation%20of%20phenolic%20compounds%20extraction%20from%20misai%20kucing%20using%20RSM%20-%20CHAP%203.pdf http://umpir.ump.edu.my/id/eprint/12750/7/Optimisation%20of%20phenolic%20compounds%20extraction%20from%20misai%20kucing%20using%20RSM%20-%20CHAP%201.pdf http://umpir.ump.edu.my/id/eprint/12750/8/Optimisation%20of%20phenolic%20compounds%20extraction%20from%20misai%20kucing%20using%20RSM%20-%2024%20Pages.pdf

id	ump-12750
recordtype	eprints
spelling	ump-127502016-11-11T07:10:06Z http://umpir.ump.edu.my/id/eprint/12750/ Optimisation of phenolic compounds extraction from misai kucing using RSM Ainin Sofia, Azni Q Science (General) TP Chemical technology Misai kucing or the scientific name is Orthosiphon stamineus contains lots of phenolic compounds such as phenolic acid, flavanoids and antioxidants. These compounds have various benefits likes antifungal, antimicrobial, antitumor and antibacterial. Besides that, people also used it as remedy to treat the diseases. In order to get components from plant materials, normally they used extraction method. The yield of phenolic compounds extraction is dependent on the size of particles, solvent used, extraction method, power irradiation and condition. The method used for this work is microwave assisted extraction was studied by using response surface methodology (RSM) design for optimization. The 2 level factorial and Box behnken design have been used to determine the optimum extraction and to develop a quadratic polynomial. The highest peak for rosmarinic acid from the extraction of misai kucing is 2.203 at 2.00 to 2.30 minutes while the standard peak of rosmarinic acid is at 2.215 at 2.00 o 2.30 minutes. The highest yield of rosmarinic acid (RA) of 24.7971 mg RA/g DW was obtained at 66.6% of aqueous ethanol concentration with 112W microwave power for 3.75 minutes. The yield for total phenolic content (TPC) and total flavonoid content (TFC) are 48.51 mg GAE/g DW and 233.243 mg QE/gDW respectively. The optimum yields were obtained at 54.68% of ethanol at 170W of microwave power for 4.34 minutes. The lower total solid (TS) was produced higher rosmarinic acid per DW of material but higher TS contain more rosmarinic acid and hence it is affect by reduced its solubility in solvent and lower extraction yield. Longer extraction time increased rosmarinic acid extraction yield at lower TS (0.1 g/ml) because longer time needed for rosmarinic acid to diffuse in the solvent. Since solvent is not saturated at lower TS so the extraction process continues and become higher at longer time. The microwave assisted extraction provides rapid extraction of phenolic compounds without significantly compromising the extraction yield. 2015-06 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/12750/1/Optimisation%20of%20phenolic%20compounds%20extraction%20from%20misai%20kucing%20using%20RSM%20-%20CHAP%203.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/12750/7/Optimisation%20of%20phenolic%20compounds%20extraction%20from%20misai%20kucing%20using%20RSM%20-%20CHAP%201.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/12750/8/Optimisation%20of%20phenolic%20compounds%20extraction%20from%20misai%20kucing%20using%20RSM%20-%2024%20Pages.pdf Ainin Sofia, Azni (2015) Optimisation of phenolic compounds extraction from misai kucing using RSM. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:93526&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English English
topic	Q Science (General) TP Chemical technology
spellingShingle	Q Science (General) TP Chemical technology Ainin Sofia, Azni Optimisation of phenolic compounds extraction from misai kucing using RSM
description	Misai kucing or the scientific name is Orthosiphon stamineus contains lots of phenolic compounds such as phenolic acid, flavanoids and antioxidants. These compounds have various benefits likes antifungal, antimicrobial, antitumor and antibacterial. Besides that, people also used it as remedy to treat the diseases. In order to get components from plant materials, normally they used extraction method. The yield of phenolic compounds extraction is dependent on the size of particles, solvent used, extraction method, power irradiation and condition. The method used for this work is microwave assisted extraction was studied by using response surface methodology (RSM) design for optimization. The 2 level factorial and Box behnken design have been used to determine the optimum extraction and to develop a quadratic polynomial. The highest peak for rosmarinic acid from the extraction of misai kucing is 2.203 at 2.00 to 2.30 minutes while the standard peak of rosmarinic acid is at 2.215 at 2.00 o 2.30 minutes. The highest yield of rosmarinic acid (RA) of 24.7971 mg RA/g DW was obtained at 66.6% of aqueous ethanol concentration with 112W microwave power for 3.75 minutes. The yield for total phenolic content (TPC) and total flavonoid content (TFC) are 48.51 mg GAE/g DW and 233.243 mg QE/gDW respectively. The optimum yields were obtained at 54.68% of ethanol at 170W of microwave power for 4.34 minutes. The lower total solid (TS) was produced higher rosmarinic acid per DW of material but higher TS contain more rosmarinic acid and hence it is affect by reduced its solubility in solvent and lower extraction yield. Longer extraction time increased rosmarinic acid extraction yield at lower TS (0.1 g/ml) because longer time needed for rosmarinic acid to diffuse in the solvent. Since solvent is not saturated at lower TS so the extraction process continues and become higher at longer time. The microwave assisted extraction provides rapid extraction of phenolic compounds without significantly compromising the extraction yield.
format	Undergraduates Project Papers
author	Ainin Sofia, Azni
author_facet	Ainin Sofia, Azni
author_sort	Ainin Sofia, Azni
title	Optimisation of phenolic compounds extraction from misai kucing using RSM
title_short	Optimisation of phenolic compounds extraction from misai kucing using RSM
title_full	Optimisation of phenolic compounds extraction from misai kucing using RSM
title_fullStr	Optimisation of phenolic compounds extraction from misai kucing using RSM
title_full_unstemmed	Optimisation of phenolic compounds extraction from misai kucing using RSM
title_sort	optimisation of phenolic compounds extraction from misai kucing using rsm
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/12750/ http://umpir.ump.edu.my/id/eprint/12750/ http://umpir.ump.edu.my/id/eprint/12750/1/Optimisation%20of%20phenolic%20compounds%20extraction%20from%20misai%20kucing%20using%20RSM%20-%20CHAP%203.pdf http://umpir.ump.edu.my/id/eprint/12750/7/Optimisation%20of%20phenolic%20compounds%20extraction%20from%20misai%20kucing%20using%20RSM%20-%20CHAP%201.pdf http://umpir.ump.edu.my/id/eprint/12750/8/Optimisation%20of%20phenolic%20compounds%20extraction%20from%20misai%20kucing%20using%20RSM%20-%2024%20Pages.pdf
first_indexed	2023-09-18T22:14:39Z
last_indexed	2023-09-18T22:14:39Z
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Optimisation of phenolic compounds extraction from misai kucing using RSM

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