Assessing storage stability and mercury reduction of freeze-dried pseudomonas putida within different types of lyoprotectant

Pseudomonas putida is a potential strain in biological treatment to remove mercury contained in the effluent of petrochemical industry due to its mercury reductase enzyme that able to reduce ionic mercury to elementary mercury. Freeze-dried P. putida allows easy, inexpensive shipping, handling and h...

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Main Author:	Yana, Nuratri
Format:	Undergraduates Project Papers
Language:	English English English
Published:	2015
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/12667/ http://umpir.ump.edu.my/id/eprint/12667/ http://umpir.ump.edu.my/id/eprint/12667/1/FKKSA%20-%20YANA%20NURATRI%20-%20CD%209583.pdf http://umpir.ump.edu.my/id/eprint/12667/2/FKKSA%20-%20YANA%20NURATRI%20-%20CD%209583%20-%20CHAP%201.pdf http://umpir.ump.edu.my/id/eprint/12667/3/FKKSA%20-%20YANA%20NURATRI%20-%20CD%209583%20-%20CHAP%203.pdf

id	ump-12667
recordtype	eprints
spelling	ump-126672016-04-05T06:55:36Z http://umpir.ump.edu.my/id/eprint/12667/ Assessing storage stability and mercury reduction of freeze-dried pseudomonas putida within different types of lyoprotectant Yana, Nuratri TP Chemical technology Pseudomonas putida is a potential strain in biological treatment to remove mercury contained in the effluent of petrochemical industry due to its mercury reductase enzyme that able to reduce ionic mercury to elementary mercury. Freeze-dried P. putida allows easy, inexpensive shipping, handling and high stability of the product. This study was aimed to freeze dry P. putida cells with addition of lyoprotectant. Lyoprotectant was added into the cells suspension prior to freezing. Dried P. putida obtained was then mixed with synthetic mercury. Viability of recovery P. putida after freeze dry was significantly influenced by the type of lyoprotectant. Among the lyoprotectants, tween 80 / sucrose was found to be the best lyoprotectant. Sucrose able to recover more than 78% (6.2E+09 CFU/ml) of the original cells (7.90E+09CFU/ml) after freeze dry and able to retain 5.40E+05 viable cells after 4 weeks storage in 4oC without vacuum. PEG pre-treated freeze dry cells and broth pre-treated freeze dry cells after freeze-dry recovered more than 64% (5.0 E+09CFU/ml) and >0.1% (5.60E+07CFU/ml). Freeze-dried P. putida cells in PEG and broth cannot survive after 4 weeks storage. Freeze dry also does not really change the pattern of growth P. putida but extension of lag time was found 1 hour after 3 weeks of storage. Additional time was required for freeze-dried P. putida cells to recover before introduce freeze-dried cells to more complicated condition such as mercury solution. The maximum mercury reduction of PEG pre-treated freeze-dried cells after freeze dry and after storage 3 weeks was 56.78% and 17.91 %.The maximum of mercury reduction of tween 80 / sucrose pre-treated freeze-dried cells after freeze dry and after storage 3 weeks were 26.35% and 25.03%. Freeze dried P .putida was found to have lower mercury reduction compare to the fresh P. putida that has been growth in agar. Result from this study may be beneficial and useful as initial reference before commercialize freeze-dried P. putida. 2015-06 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/12667/1/FKKSA%20-%20YANA%20NURATRI%20-%20CD%209583.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/12667/2/FKKSA%20-%20YANA%20NURATRI%20-%20CD%209583%20-%20CHAP%201.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/12667/3/FKKSA%20-%20YANA%20NURATRI%20-%20CD%209583%20-%20CHAP%203.pdf Yana, Nuratri (2015) Assessing storage stability and mercury reduction of freeze-dried pseudomonas putida within different types of lyoprotectant. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:93770&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Yana, Nuratri Assessing storage stability and mercury reduction of freeze-dried pseudomonas putida within different types of lyoprotectant
description	Pseudomonas putida is a potential strain in biological treatment to remove mercury contained in the effluent of petrochemical industry due to its mercury reductase enzyme that able to reduce ionic mercury to elementary mercury. Freeze-dried P. putida allows easy, inexpensive shipping, handling and high stability of the product. This study was aimed to freeze dry P. putida cells with addition of lyoprotectant. Lyoprotectant was added into the cells suspension prior to freezing. Dried P. putida obtained was then mixed with synthetic mercury. Viability of recovery P. putida after freeze dry was significantly influenced by the type of lyoprotectant. Among the lyoprotectants, tween 80 / sucrose was found to be the best lyoprotectant. Sucrose able to recover more than 78% (6.2E+09 CFU/ml) of the original cells (7.90E+09CFU/ml) after freeze dry and able to retain 5.40E+05 viable cells after 4 weeks storage in 4oC without vacuum. PEG pre-treated freeze dry cells and broth pre-treated freeze dry cells after freeze-dry recovered more than 64% (5.0 E+09CFU/ml) and >0.1% (5.60E+07CFU/ml). Freeze-dried P. putida cells in PEG and broth cannot survive after 4 weeks storage. Freeze dry also does not really change the pattern of growth P. putida but extension of lag time was found 1 hour after 3 weeks of storage. Additional time was required for freeze-dried P. putida cells to recover before introduce freeze-dried cells to more complicated condition such as mercury solution. The maximum mercury reduction of PEG pre-treated freeze-dried cells after freeze dry and after storage 3 weeks was 56.78% and 17.91 %.The maximum of mercury reduction of tween 80 / sucrose pre-treated freeze-dried cells after freeze dry and after storage 3 weeks were 26.35% and 25.03%. Freeze dried P .putida was found to have lower mercury reduction compare to the fresh P. putida that has been growth in agar. Result from this study may be beneficial and useful as initial reference before commercialize freeze-dried P. putida.
format	Undergraduates Project Papers
author	Yana, Nuratri
author_facet	Yana, Nuratri
author_sort	Yana, Nuratri
title	Assessing storage stability and mercury reduction of freeze-dried pseudomonas putida within different types of lyoprotectant
title_short	Assessing storage stability and mercury reduction of freeze-dried pseudomonas putida within different types of lyoprotectant
title_full	Assessing storage stability and mercury reduction of freeze-dried pseudomonas putida within different types of lyoprotectant
title_fullStr	Assessing storage stability and mercury reduction of freeze-dried pseudomonas putida within different types of lyoprotectant
title_full_unstemmed	Assessing storage stability and mercury reduction of freeze-dried pseudomonas putida within different types of lyoprotectant
title_sort	assessing storage stability and mercury reduction of freeze-dried pseudomonas putida within different types of lyoprotectant
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/12667/ http://umpir.ump.edu.my/id/eprint/12667/ http://umpir.ump.edu.my/id/eprint/12667/1/FKKSA%20-%20YANA%20NURATRI%20-%20CD%209583.pdf http://umpir.ump.edu.my/id/eprint/12667/2/FKKSA%20-%20YANA%20NURATRI%20-%20CD%209583%20-%20CHAP%201.pdf http://umpir.ump.edu.my/id/eprint/12667/3/FKKSA%20-%20YANA%20NURATRI%20-%20CD%209583%20-%20CHAP%203.pdf
first_indexed	2023-09-18T22:14:31Z
last_indexed	2023-09-18T22:14:31Z
_version_	1777415247520333824

Assessing storage stability and mercury reduction of freeze-dried pseudomonas putida within different types of lyoprotectant

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