Proteomic Analysis of Differentially Expressed Protein in Hemocytes of Wild Giant Freshwater Prawn Macrobrachium Rosenbergii Infected with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV)

Epizootic diseases cause huge mortality and economical loses at post larvae stages in freshwater prawn aquaculture industry. These prawns seem less susceptible to viral diseases except for infectious hypodermal and hematopoietic necrosis virus (IHHNV). During viral infection in prawns, hemocytes are...

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Main Authors:	Alinejad, T., Kwong, Q. Bin, Jaya Vejayan, Palliah, Othman, R. Y., Bhassu, S.
Format:	Article
Language:	English
Published:	Elsevier 2015
Subjects:	QP Physiology
Online Access:	http://umpir.ump.edu.my/id/eprint/12636/ http://umpir.ump.edu.my/id/eprint/12636/ http://umpir.ump.edu.my/id/eprint/12636/ http://umpir.ump.edu.my/id/eprint/12636/1/fist-2015-jaya-Proteomic%20Analysis%20of%20Differentially%20Expressed.pdf

id	ump-12636
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spelling	ump-126362018-02-21T06:55:54Z http://umpir.ump.edu.my/id/eprint/12636/ Proteomic Analysis of Differentially Expressed Protein in Hemocytes of Wild Giant Freshwater Prawn Macrobrachium Rosenbergii Infected with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) Alinejad, T. Kwong, Q. Bin Jaya Vejayan, Palliah Othman, R. Y. Bhassu, S. QP Physiology Epizootic diseases cause huge mortality and economical loses at post larvae stages in freshwater prawn aquaculture industry. These prawns seem less susceptible to viral diseases except for infectious hypodermal and hematopoietic necrosis virus (IHHNV). During viral infection in prawns, hemocytes are the primary organ that shows immunological response within the early stages of infection. We applied proteomic approaches to understand differential expression of the proteins in hemocytes during the viral disease outbreak. To aid the goal, we collected Macrobrachium rosenbergii broodstocks from the local grow out hatchery which reported the first incidence of IHHNV viral outbreak during larvae stage. Primarily, application of the OIE primer targeting 389 bp fragments of IHHNV virus was used in identification of the infected and non-infected samples of the prawn breeding line. Analysis of two-dimensional gel electrophoresis showed specific down-regulation of Arginine kinase and Sarcoplasmic calcium-binding protein and up/down-regulation of Prophenoloxidase1 and hemocyanin isoforms. These proteins were validated using semi quantitative RT-PCR and gene transcripts at mRNA level. These identified proteins can be used as biomarkers, providing a powerful approach to better understanding of the immunity pathway of viral disease with applications in analytic and observational epidemiology diagnosis. Proteomic profiling allows deep insight into the pathogenesis of IHHNV molecular regulation and mechanism of hemocyte in freshwater prawns Elsevier 2015 Article PeerReviewed application/pdf en cc_by_nc_nd http://umpir.ump.edu.my/id/eprint/12636/1/fist-2015-jaya-Proteomic%20Analysis%20of%20Differentially%20Expressed.pdf Alinejad, T. and Kwong, Q. Bin and Jaya Vejayan, Palliah and Othman, R. Y. and Bhassu, S. (2015) Proteomic Analysis of Differentially Expressed Protein in Hemocytes of Wild Giant Freshwater Prawn Macrobrachium Rosenbergii Infected with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV). Meta Gene, 5 (Sept.). pp. 55-67. ISSN 2214-5400 http://dx.doi.org/10.1016/j.mgene.2015.05.004 DOI: 10.1016/j.mgene.2015.05.004
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QP Physiology
spellingShingle	QP Physiology Alinejad, T. Kwong, Q. Bin Jaya Vejayan, Palliah Othman, R. Y. Bhassu, S. Proteomic Analysis of Differentially Expressed Protein in Hemocytes of Wild Giant Freshwater Prawn Macrobrachium Rosenbergii Infected with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV)
description	Epizootic diseases cause huge mortality and economical loses at post larvae stages in freshwater prawn aquaculture industry. These prawns seem less susceptible to viral diseases except for infectious hypodermal and hematopoietic necrosis virus (IHHNV). During viral infection in prawns, hemocytes are the primary organ that shows immunological response within the early stages of infection. We applied proteomic approaches to understand differential expression of the proteins in hemocytes during the viral disease outbreak. To aid the goal, we collected Macrobrachium rosenbergii broodstocks from the local grow out hatchery which reported the first incidence of IHHNV viral outbreak during larvae stage. Primarily, application of the OIE primer targeting 389 bp fragments of IHHNV virus was used in identification of the infected and non-infected samples of the prawn breeding line. Analysis of two-dimensional gel electrophoresis showed specific down-regulation of Arginine kinase and Sarcoplasmic calcium-binding protein and up/down-regulation of Prophenoloxidase1 and hemocyanin isoforms. These proteins were validated using semi quantitative RT-PCR and gene transcripts at mRNA level. These identified proteins can be used as biomarkers, providing a powerful approach to better understanding of the immunity pathway of viral disease with applications in analytic and observational epidemiology diagnosis. Proteomic profiling allows deep insight into the pathogenesis of IHHNV molecular regulation and mechanism of hemocyte in freshwater prawns
format	Article
author	Alinejad, T. Kwong, Q. Bin Jaya Vejayan, Palliah Othman, R. Y. Bhassu, S.
author_facet	Alinejad, T. Kwong, Q. Bin Jaya Vejayan, Palliah Othman, R. Y. Bhassu, S.
author_sort	Alinejad, T.
title	Proteomic Analysis of Differentially Expressed Protein in Hemocytes of Wild Giant Freshwater Prawn Macrobrachium Rosenbergii Infected with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV)
title_short	Proteomic Analysis of Differentially Expressed Protein in Hemocytes of Wild Giant Freshwater Prawn Macrobrachium Rosenbergii Infected with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV)
title_full	Proteomic Analysis of Differentially Expressed Protein in Hemocytes of Wild Giant Freshwater Prawn Macrobrachium Rosenbergii Infected with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV)
title_fullStr	Proteomic Analysis of Differentially Expressed Protein in Hemocytes of Wild Giant Freshwater Prawn Macrobrachium Rosenbergii Infected with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV)
title_full_unstemmed	Proteomic Analysis of Differentially Expressed Protein in Hemocytes of Wild Giant Freshwater Prawn Macrobrachium Rosenbergii Infected with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV)
title_sort	proteomic analysis of differentially expressed protein in hemocytes of wild giant freshwater prawn macrobrachium rosenbergii infected with infectious hypodermal and hematopoietic necrosis virus (ihhnv)
publisher	Elsevier
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/12636/ http://umpir.ump.edu.my/id/eprint/12636/ http://umpir.ump.edu.my/id/eprint/12636/ http://umpir.ump.edu.my/id/eprint/12636/1/fist-2015-jaya-Proteomic%20Analysis%20of%20Differentially%20Expressed.pdf
first_indexed	2023-09-18T22:14:28Z
last_indexed	2023-09-18T22:14:28Z
_version_	1777415244465831936

Proteomic Analysis of Differentially Expressed Protein in Hemocytes of Wild Giant Freshwater Prawn Macrobrachium Rosenbergii Infected with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV)

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