A Factorial Analysis Study on Enzymatic Hydrolysis of Fiber Pressed Oil Palm Frond for Bioethanol Production
Different technologies have been developed to for the conversion of lignocellulosic biomass to suitable fermentation substrates for bioethanol production. The enzymatic conversion of cellulose seems to be the most promising technology as it is highly specific and does not produce substantial amounts...
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2016
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ump-120242018-02-19T05:19:10Z http://umpir.ump.edu.my/id/eprint/12024/ A Factorial Analysis Study on Enzymatic Hydrolysis of Fiber Pressed Oil Palm Frond for Bioethanol Production Fatin Syazwana, Hashim H. W., Yussof Mior Ahmad Khushairi, Mohd Zahari Rosli Md, Illias R. A., Rahman TP Chemical technology Different technologies have been developed to for the conversion of lignocellulosic biomass to suitable fermentation substrates for bioethanol production. The enzymatic conversion of cellulose seems to be the most promising technology as it is highly specific and does not produce substantial amounts of unwanted byproducts. The effects of agitation speed, enzyme loading, temperature, pH and reaction time on the conversion of glucose from fiber pressed oil palm frond (FPOPF) for bioethanol production were screened by statistical analysis using response surface methodology (RSM). A half fraction two-level factorial analysis with five factors was selected for the experimental design to determine the best enzymatic conditions that produce maximum amount of glucose. FPOPF was pre-treated with alkaline prior to enzymatic hydrolysis. The enzymatic hydrolysis was performed using a commercial enzyme Cellic CTec2. From this study, the highest yield of glucose concentration was 9.736 g/L at 72 hours reaction time at 35 °C, pH 5.6, and 1.5% (w/v) of enzyme loading. The model obtained was significant with p-value <0.0001. It is suggested that this model had a maximum point which is likely to be the optimum point and possible for the optimization process. IOP Publishing 2016 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/12024/1/A%20Factorial%20Analysis%20Study%20on%20Enzymatic%20Hydrolysis%20of%20Fiber.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/12024/7/A%20Factorial%20Analysis%20Study%20on%20Enzymatic%20Hydrolysis%20of%20Fiber-Abstract.pdf Fatin Syazwana, Hashim and H. W., Yussof and Mior Ahmad Khushairi, Mohd Zahari and Rosli Md, Illias and R. A., Rahman (2016) A Factorial Analysis Study on Enzymatic Hydrolysis of Fiber Pressed Oil Palm Frond for Bioethanol Production. In: IOP Conference Series: Earth and Environmental Science, International Conference on Advances in Renewable Energy and Technologies (ICARET 2016), 23-25 February 2016 , Putrajaya, Malaysia. pp. 1-6., 32 (012071). ISSN 1755-1307 (Print); 1755-1315 (Online) http://dx.doi.org/10.1088/1755-1315/32/1/012071 |
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TP Chemical technology Fatin Syazwana, Hashim H. W., Yussof Mior Ahmad Khushairi, Mohd Zahari Rosli Md, Illias R. A., Rahman A Factorial Analysis Study on Enzymatic Hydrolysis of Fiber Pressed Oil Palm Frond for Bioethanol Production |
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Different technologies have been developed to for the conversion of lignocellulosic biomass to suitable fermentation substrates for bioethanol production. The enzymatic conversion of cellulose seems to be the most promising technology as it is highly specific and does not produce substantial amounts of unwanted byproducts. The effects of agitation speed, enzyme loading, temperature, pH and reaction time on the conversion of glucose from fiber
pressed oil palm frond (FPOPF) for bioethanol production were screened by statistical analysis using response surface methodology (RSM). A half fraction two-level factorial analysis with five factors was selected for the experimental design to determine the best enzymatic conditions that produce maximum amount of glucose. FPOPF was pre-treated with alkaline prior to enzymatic hydrolysis. The enzymatic hydrolysis was performed using a commercial enzyme Cellic CTec2. From this study, the highest yield of glucose concentration was 9.736 g/L at 72 hours reaction time at 35 °C, pH 5.6, and 1.5% (w/v) of enzyme loading. The model obtained was significant with p-value <0.0001. It is suggested that this model had a maximum point which is likely to be the optimum point and possible for the optimization process. |
format |
Conference or Workshop Item |
author |
Fatin Syazwana, Hashim H. W., Yussof Mior Ahmad Khushairi, Mohd Zahari Rosli Md, Illias R. A., Rahman |
author_facet |
Fatin Syazwana, Hashim H. W., Yussof Mior Ahmad Khushairi, Mohd Zahari Rosli Md, Illias R. A., Rahman |
author_sort |
Fatin Syazwana, Hashim |
title |
A Factorial Analysis Study on Enzymatic Hydrolysis of Fiber Pressed Oil Palm Frond for Bioethanol Production |
title_short |
A Factorial Analysis Study on Enzymatic Hydrolysis of Fiber Pressed Oil Palm Frond for Bioethanol Production |
title_full |
A Factorial Analysis Study on Enzymatic Hydrolysis of Fiber Pressed Oil Palm Frond for Bioethanol Production |
title_fullStr |
A Factorial Analysis Study on Enzymatic Hydrolysis of Fiber Pressed Oil Palm Frond for Bioethanol Production |
title_full_unstemmed |
A Factorial Analysis Study on Enzymatic Hydrolysis of Fiber Pressed Oil Palm Frond for Bioethanol Production |
title_sort |
factorial analysis study on enzymatic hydrolysis of fiber pressed oil palm frond for bioethanol production |
publisher |
IOP Publishing |
publishDate |
2016 |
url |
http://umpir.ump.edu.my/id/eprint/12024/ http://umpir.ump.edu.my/id/eprint/12024/ http://umpir.ump.edu.my/id/eprint/12024/1/A%20Factorial%20Analysis%20Study%20on%20Enzymatic%20Hydrolysis%20of%20Fiber.pdf http://umpir.ump.edu.my/id/eprint/12024/7/A%20Factorial%20Analysis%20Study%20on%20Enzymatic%20Hydrolysis%20of%20Fiber-Abstract.pdf |
first_indexed |
2023-09-18T22:13:12Z |
last_indexed |
2023-09-18T22:13:12Z |
_version_ |
1777415164650323968 |