Intrusion of Polyethylene Glycol during Osmotic Tests: Identifying Cellulose Acetate Degrading Microbes

Cellulose acetate semipermeable membranes and polyethylene glycol (PEG) solutions are commonly used to apply suction in soils using the osmotic technique. The structural integrity of the membrane is crucial to maintain a consistent suction value throughout a test. The membrane however, is vulnerable...

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Main Authors:	Mohd Yuhyi, Mohd Tadza, Mohd Fazli, Farida Asras, Muhammad Afiq, Mohd Tadza, Jamil, Ismail, Nurhidayah, Mahazam, Nurul Shafiqah, Mohd Azmi
Format:	Article
Language:	English
Published:	Penerbit Universiti Teknologi Malaysia 2016
Subjects:	Q Science (General) TA Engineering (General). Civil engineering (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/12022/ http://umpir.ump.edu.my/id/eprint/12022/ http://umpir.ump.edu.my/id/eprint/12022/ http://umpir.ump.edu.my/id/eprint/12022/1/Intrusion%20Of%20Polyethylene%20Glycol%20During%20Osmotic%20Tests-%20Identifying%20Cellulose%20Acetate%20Degrading%20Microbes.pdf

id	ump-12022
recordtype	eprints
spelling	ump-120222019-10-18T02:55:23Z http://umpir.ump.edu.my/id/eprint/12022/ Intrusion of Polyethylene Glycol during Osmotic Tests: Identifying Cellulose Acetate Degrading Microbes Mohd Yuhyi, Mohd Tadza Mohd Fazli, Farida Asras Muhammad Afiq, Mohd Tadza Jamil, Ismail Nurhidayah, Mahazam Nurul Shafiqah, Mohd Azmi Q Science (General) TA Engineering (General). Civil engineering (General) Cellulose acetate semipermeable membranes and polyethylene glycol (PEG) solutions are commonly used to apply suction in soils using the osmotic technique. The structural integrity of the membrane is crucial to maintain a consistent suction value throughout a test. The membrane however, is vulnerable to microbial attack, which in turn could lead to intrusion of PEG into soil specimens. In this study, osmotic test was carried out on initially saturated Andrassy bentonite specimen. PEG 6000 and membrane with molecular weight cut-off (MWCO) value of 3500 was used to apply suction of 3.4 MPa. Soil specimen and PEG solution after the osmotic test were examined for the presence of any potential cellulose or acetate degrading microbes. Test results indicated that both cellulose degrading bacteria and fungi were present in the PEG solutions. Addition of penicillin was found to be less effective in removing these microbes. However, 70% ethanol may be used to prevent cross contamination during handling of specimens. It is anticipated that eliminating these microbes is crucial to prevent intrusion of PEG in osmotic tests. Penerbit Universiti Teknologi Malaysia 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/12022/1/Intrusion%20Of%20Polyethylene%20Glycol%20During%20Osmotic%20Tests-%20Identifying%20Cellulose%20Acetate%20Degrading%20Microbes.pdf Mohd Yuhyi, Mohd Tadza and Mohd Fazli, Farida Asras and Muhammad Afiq, Mohd Tadza and Jamil, Ismail and Nurhidayah, Mahazam and Nurul Shafiqah, Mohd Azmi (2016) Intrusion of Polyethylene Glycol during Osmotic Tests: Identifying Cellulose Acetate Degrading Microbes. Jurnal Teknologi (Sciences and Engineering), 78 (8-5). pp. 11-16. ISSN 0127-9696 (print); 2180-3722 (online) http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/9604 DOI: 10.11113/jt.v78.9604
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General) TA Engineering (General). Civil engineering (General)
spellingShingle	Q Science (General) TA Engineering (General). Civil engineering (General) Mohd Yuhyi, Mohd Tadza Mohd Fazli, Farida Asras Muhammad Afiq, Mohd Tadza Jamil, Ismail Nurhidayah, Mahazam Nurul Shafiqah, Mohd Azmi Intrusion of Polyethylene Glycol during Osmotic Tests: Identifying Cellulose Acetate Degrading Microbes
description	Cellulose acetate semipermeable membranes and polyethylene glycol (PEG) solutions are commonly used to apply suction in soils using the osmotic technique. The structural integrity of the membrane is crucial to maintain a consistent suction value throughout a test. The membrane however, is vulnerable to microbial attack, which in turn could lead to intrusion of PEG into soil specimens. In this study, osmotic test was carried out on initially saturated Andrassy bentonite specimen. PEG 6000 and membrane with molecular weight cut-off (MWCO) value of 3500 was used to apply suction of 3.4 MPa. Soil specimen and PEG solution after the osmotic test were examined for the presence of any potential cellulose or acetate degrading microbes. Test results indicated that both cellulose degrading bacteria and fungi were present in the PEG solutions. Addition of penicillin was found to be less effective in removing these microbes. However, 70% ethanol may be used to prevent cross contamination during handling of specimens. It is anticipated that eliminating these microbes is crucial to prevent intrusion of PEG in osmotic tests.
format	Article
author	Mohd Yuhyi, Mohd Tadza Mohd Fazli, Farida Asras Muhammad Afiq, Mohd Tadza Jamil, Ismail Nurhidayah, Mahazam Nurul Shafiqah, Mohd Azmi
author_facet	Mohd Yuhyi, Mohd Tadza Mohd Fazli, Farida Asras Muhammad Afiq, Mohd Tadza Jamil, Ismail Nurhidayah, Mahazam Nurul Shafiqah, Mohd Azmi
author_sort	Mohd Yuhyi, Mohd Tadza
title	Intrusion of Polyethylene Glycol during Osmotic Tests: Identifying Cellulose Acetate Degrading Microbes
title_short	Intrusion of Polyethylene Glycol during Osmotic Tests: Identifying Cellulose Acetate Degrading Microbes
title_full	Intrusion of Polyethylene Glycol during Osmotic Tests: Identifying Cellulose Acetate Degrading Microbes
title_fullStr	Intrusion of Polyethylene Glycol during Osmotic Tests: Identifying Cellulose Acetate Degrading Microbes
title_full_unstemmed	Intrusion of Polyethylene Glycol during Osmotic Tests: Identifying Cellulose Acetate Degrading Microbes
title_sort	intrusion of polyethylene glycol during osmotic tests: identifying cellulose acetate degrading microbes
publisher	Penerbit Universiti Teknologi Malaysia
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/12022/ http://umpir.ump.edu.my/id/eprint/12022/ http://umpir.ump.edu.my/id/eprint/12022/ http://umpir.ump.edu.my/id/eprint/12022/1/Intrusion%20Of%20Polyethylene%20Glycol%20During%20Osmotic%20Tests-%20Identifying%20Cellulose%20Acetate%20Degrading%20Microbes.pdf
first_indexed	2023-09-18T22:13:11Z
last_indexed	2023-09-18T22:13:11Z
_version_	1777415164499329024

Intrusion of Polyethylene Glycol during Osmotic Tests: Identifying Cellulose Acetate Degrading Microbes

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