Production and Scale-Up of a Monoclonal Antibody Against 17-Hydroxyprogesterone

The hybridoma 192 was used to produce a monoclonal antibody (MAb) against 17-hydroxyprogesterone (17-OHP), for possible use in screening for congenital adrenal hyperplasia (CAH). The factors influencing the MAb production were screened and optimized in a 2 L stirred bioreactor. The production was th...

Full description

Bibliographic Details
Main Authors:	Chua, Gek Kee, Abdul-Rahman, Badarulhisam, Chisti, Yusuf
Format:	Article
Language:	English
Published:	Wiley 2013
Subjects:	QR180 Immunology RM Therapeutics. Pharmacology TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/11957/ http://umpir.ump.edu.my/id/eprint/11957/ http://umpir.ump.edu.my/id/eprint/11957/ http://umpir.ump.edu.my/id/eprint/11957/1/Production%20and%20Scale-up%20of%20a%20Monoclonal%20Antibody%20Against%2017-Hydroxyprogesterone.pdf

id	ump-11957
recordtype	eprints
spelling	ump-119572017-12-15T04:05:04Z http://umpir.ump.edu.my/id/eprint/11957/ Production and Scale-Up of a Monoclonal Antibody Against 17-Hydroxyprogesterone Chua, Gek Kee Abdul-Rahman, Badarulhisam Chisti, Yusuf QR180 Immunology RM Therapeutics. Pharmacology TP Chemical technology The hybridoma 192 was used to produce a monoclonal antibody (MAb) against 17-hydroxyprogesterone (17-OHP), for possible use in screening for congenital adrenal hyperplasia (CAH). The factors influencing the MAb production were screened and optimized in a 2 L stirred bioreactor. The production was then scaled up to a 20 L bioreactor. All of the screened factors (aeration rate, stirring speed, dissolved oxygen concentration, pH, and temperature) were found to significantly affect production. Optimization using the response surface methodology identified the following optimal production conditions: 36.8°C, pH 7.4, stirring speed of 100 rpm, 30% dissolved oxygen concentration, and an aeration rate of 0.09 vvm. Under these conditions, the maximum viable cell density achieved was 1.34 ± 0.21 × 10(6) cells mL(-1) and the specific growth rate was 0.036 ± 0.004 h(-1) . The maximum MAb titer was 11.94 ± 4.81 μg mL(-1) with an average specific MAb production rate of 0.273 ± 0.135 pg cell(-1) h(-1) . A constant impeller tip speed criterion was used for the scale-up. The specific growth rate (0.040 h(-1) ) and the maximum viable cell density (1.89 × 10(6) cells mL(-1) ) at the larger scale were better than the values achieved at the small scale, but the MAb titer in the 20 L bioreactor was 18% lower than in the smaller bioreactor. A change in the culture environment from the static conditions of a T-flask to the stirred bioreactor culture did not affect the specificity of the MAb toward its antigen (17-OHP) and did not compromise the structural integrity of the MAb. Wiley 2013 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/11957/1/Production%20and%20Scale-up%20of%20a%20Monoclonal%20Antibody%20Against%2017-Hydroxyprogesterone.pdf Chua, Gek Kee and Abdul-Rahman, Badarulhisam and Chisti, Yusuf (2013) Production and Scale-Up of a Monoclonal Antibody Against 17-Hydroxyprogesterone. Biotechnology Progress, 29 (1). pp. 154-164. ISSN 1520-6033 http://dx.doi.org/10.1002/btpr.1656 DOI: 10.1002/btpr.1656
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QR180 Immunology RM Therapeutics. Pharmacology TP Chemical technology
spellingShingle	QR180 Immunology RM Therapeutics. Pharmacology TP Chemical technology Chua, Gek Kee Abdul-Rahman, Badarulhisam Chisti, Yusuf Production and Scale-Up of a Monoclonal Antibody Against 17-Hydroxyprogesterone
description	The hybridoma 192 was used to produce a monoclonal antibody (MAb) against 17-hydroxyprogesterone (17-OHP), for possible use in screening for congenital adrenal hyperplasia (CAH). The factors influencing the MAb production were screened and optimized in a 2 L stirred bioreactor. The production was then scaled up to a 20 L bioreactor. All of the screened factors (aeration rate, stirring speed, dissolved oxygen concentration, pH, and temperature) were found to significantly affect production. Optimization using the response surface methodology identified the following optimal production conditions: 36.8°C, pH 7.4, stirring speed of 100 rpm, 30% dissolved oxygen concentration, and an aeration rate of 0.09 vvm. Under these conditions, the maximum viable cell density achieved was 1.34 ± 0.21 × 10(6) cells mL(-1) and the specific growth rate was 0.036 ± 0.004 h(-1) . The maximum MAb titer was 11.94 ± 4.81 μg mL(-1) with an average specific MAb production rate of 0.273 ± 0.135 pg cell(-1) h(-1) . A constant impeller tip speed criterion was used for the scale-up. The specific growth rate (0.040 h(-1) ) and the maximum viable cell density (1.89 × 10(6) cells mL(-1) ) at the larger scale were better than the values achieved at the small scale, but the MAb titer in the 20 L bioreactor was 18% lower than in the smaller bioreactor. A change in the culture environment from the static conditions of a T-flask to the stirred bioreactor culture did not affect the specificity of the MAb toward its antigen (17-OHP) and did not compromise the structural integrity of the MAb.
format	Article
author	Chua, Gek Kee Abdul-Rahman, Badarulhisam Chisti, Yusuf
author_facet	Chua, Gek Kee Abdul-Rahman, Badarulhisam Chisti, Yusuf
author_sort	Chua, Gek Kee
title	Production and Scale-Up of a Monoclonal Antibody Against 17-Hydroxyprogesterone
title_short	Production and Scale-Up of a Monoclonal Antibody Against 17-Hydroxyprogesterone
title_full	Production and Scale-Up of a Monoclonal Antibody Against 17-Hydroxyprogesterone
title_fullStr	Production and Scale-Up of a Monoclonal Antibody Against 17-Hydroxyprogesterone
title_full_unstemmed	Production and Scale-Up of a Monoclonal Antibody Against 17-Hydroxyprogesterone
title_sort	production and scale-up of a monoclonal antibody against 17-hydroxyprogesterone
publisher	Wiley
publishDate	2013
url	http://umpir.ump.edu.my/id/eprint/11957/ http://umpir.ump.edu.my/id/eprint/11957/ http://umpir.ump.edu.my/id/eprint/11957/ http://umpir.ump.edu.my/id/eprint/11957/1/Production%20and%20Scale-up%20of%20a%20Monoclonal%20Antibody%20Against%2017-Hydroxyprogesterone.pdf
first_indexed	2023-09-18T22:13:04Z
last_indexed	2023-09-18T22:13:04Z
_version_	1777415156337213440

Production and Scale-Up of a Monoclonal Antibody Against 17-Hydroxyprogesterone

Similar Items