Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White

Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White

Membrane chromatography, which uses an adsorptive membrane, is an alternative technology that overcomes the limitations of packed-bed column chromatography. Mixed matrix membrane (MMM) preparation is a simple method by which adsorptive membranes can be prepared. In the current study, several potenti...

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Main Authors: N., Shamsinar, Syed Mohd Saufi, Tuan Chik
Format: Article
Language:English
Published: Springer 2016
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/11899/
http://umpir.ump.edu.my/id/eprint/11899/
http://umpir.ump.edu.my/id/eprint/11899/
http://umpir.ump.edu.my/id/eprint/11899/1/Adsorptive%20Cation%20Exchanger%20Mixed%20Matrix%20Membrane%20Chromatography%20for%20the%20Isolation%20of%20Lysozyme%20from%20Chicken%20Egg%20White.pdf
id ump-11899
recordtype eprints
spelling ump-118992018-09-06T07:22:19Z http://umpir.ump.edu.my/id/eprint/11899/ Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White N., Shamsinar Syed Mohd Saufi, Tuan Chik TP Chemical technology Membrane chromatography, which uses an adsorptive membrane, is an alternative technology that overcomes the limitations of packed-bed column chromatography. Mixed matrix membrane (MMM) preparation is a simple method by which adsorptive membranes can be prepared. In the current study, several potential low-cost cation exchange (CEX) resins were screened for their ability to bind lysozyme (LYS) at various pH values prior to the preparation of CEX MMMs. The best binding was shown by Lewatit CNP105 at pH 7. A subsequently prepared Lewatit CNP105—CEX MMM followed the Langmuir adsorption isotherm, with a maximum binding capacity of 223 mg LYS/g membrane. In batch binding from a chicken egg white (CEW) solution at pH 7, both positively charged (LYS, pI 10.7) and negatively charged (conalbumin (CNL), pI 6.1; and ovalbumin (OVL), pI 4.5) proteins were bound onto the MMM. However, CNL and OVL were only loosely bound and were washed out during the washing step. Almost pure LYS was recovered during the elution step, as shown by SDS–PAGE gel analysis. Springer 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/11899/1/Adsorptive%20Cation%20Exchanger%20Mixed%20Matrix%20Membrane%20Chromatography%20for%20the%20Isolation%20of%20Lysozyme%20from%20Chicken%20Egg%20White.pdf N., Shamsinar and Syed Mohd Saufi, Tuan Chik (2016) Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White. Arabian Journal for Science and Engineering. pp. 1-7. ISSN 1319-8025 (print); 2191-4281 (online) http://dx.doi.org/10.1007/s13369-015-1939-8 DOI: 10.1007/s13369-015-1939-8
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
N., Shamsinar
Syed Mohd Saufi, Tuan Chik
Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White
description Membrane chromatography, which uses an adsorptive membrane, is an alternative technology that overcomes the limitations of packed-bed column chromatography. Mixed matrix membrane (MMM) preparation is a simple method by which adsorptive membranes can be prepared. In the current study, several potential low-cost cation exchange (CEX) resins were screened for their ability to bind lysozyme (LYS) at various pH values prior to the preparation of CEX MMMs. The best binding was shown by Lewatit CNP105 at pH 7. A subsequently prepared Lewatit CNP105—CEX MMM followed the Langmuir adsorption isotherm, with a maximum binding capacity of 223 mg LYS/g membrane. In batch binding from a chicken egg white (CEW) solution at pH 7, both positively charged (LYS, pI 10.7) and negatively charged (conalbumin (CNL), pI 6.1; and ovalbumin (OVL), pI 4.5) proteins were bound onto the MMM. However, CNL and OVL were only loosely bound and were washed out during the washing step. Almost pure LYS was recovered during the elution step, as shown by SDS–PAGE gel analysis.
format Article
author N., Shamsinar
Syed Mohd Saufi, Tuan Chik
author_facet N., Shamsinar
Syed Mohd Saufi, Tuan Chik
author_sort N., Shamsinar
title Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White
title_short Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White
title_full Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White
title_fullStr Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White
title_full_unstemmed Adsorptive Cation Exchanger Mixed Matrix Membrane Chromatography for the Isolation of Lysozyme from Chicken Egg White
title_sort adsorptive cation exchanger mixed matrix membrane chromatography for the isolation of lysozyme from chicken egg white
publisher Springer
publishDate 2016
url http://umpir.ump.edu.my/id/eprint/11899/
http://umpir.ump.edu.my/id/eprint/11899/
http://umpir.ump.edu.my/id/eprint/11899/
http://umpir.ump.edu.my/id/eprint/11899/1/Adsorptive%20Cation%20Exchanger%20Mixed%20Matrix%20Membrane%20Chromatography%20for%20the%20Isolation%20of%20Lysozyme%20from%20Chicken%20Egg%20White.pdf
first_indexed 2023-09-18T22:12:57Z
last_indexed 2023-09-18T22:12:57Z
_version_ 1777415149141884928

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