Application of Enzyme Coupling Reactions to Shift Thermodynamically Limited Biocatalytic Reactions

In recent years, much interest has been shown in the use of multi-enzyme cascades as a tool in organic synthesis. Such enzymatic cascades can provide added value to a synthetic scheme by starting from cheaper raw materials or making more valuable products. Additionally, they can be used to help shif...

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Main Authors:	Rohana, Abu, Woodley, John M.
Format:	Article
Language:	English
Published:	Wiley-VCH Verlag GmbH & Co KGaA 2015
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/11812/ http://umpir.ump.edu.my/id/eprint/11812/ http://umpir.ump.edu.my/id/eprint/11812/ http://umpir.ump.edu.my/id/eprint/11812/1/Application%20of%20Enzyme%20Coupling%20Reactions%20to%20Shift%20Thermodynamically%20Limited%20Biocatalytic%20Reactions.pdf

id	ump-11812
recordtype	eprints
spelling	ump-118122018-05-15T07:21:25Z http://umpir.ump.edu.my/id/eprint/11812/ Application of Enzyme Coupling Reactions to Shift Thermodynamically Limited Biocatalytic Reactions Rohana, Abu Woodley, John M. TP Chemical technology In recent years, much interest has been shown in the use of multi-enzyme cascades as a tool in organic synthesis. Such enzymatic cascades can provide added value to a synthetic scheme by starting from cheaper raw materials or making more valuable products. Additionally, they can be used to help shift the equilibrium of otherwise thermodynamically unfavourable reactions to give a higher conversion of the target product. By coupling an energetically unfavourable reaction with a more favourable one, the multi-enzyme cascade mimics the approach taken in nature in metabolic pathways. Nevertheless, it can be challenging to combine several engineered enzymes in vitro for the conversion of non-natural substrates. In this mini-review we focus on enzyme coupling reactions as a tool to alleviate thermodynamic constraints in synthetically useful biocatalytic reactions. The implications of thermodynamic parameters such as the equilibrium constant on the multi-enzyme cascades and the conventional methods of equilibrium shifting are also discussed in addition to methods used to estimate such values. Wiley-VCH Verlag GmbH & Co KGaA 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/11812/1/Application%20of%20Enzyme%20Coupling%20Reactions%20to%20Shift%20Thermodynamically%20Limited%20Biocatalytic%20Reactions.pdf Rohana, Abu and Woodley, John M. (2015) Application of Enzyme Coupling Reactions to Shift Thermodynamically Limited Biocatalytic Reactions. ChemCatChem, 7 (19). pp. 3094-3105. ISSN 1867-3899 http://dx.doi.org/10.1002/cctc.201500603 DOI: 10.1002/cctc.201500603
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Rohana, Abu Woodley, John M. Application of Enzyme Coupling Reactions to Shift Thermodynamically Limited Biocatalytic Reactions
description	In recent years, much interest has been shown in the use of multi-enzyme cascades as a tool in organic synthesis. Such enzymatic cascades can provide added value to a synthetic scheme by starting from cheaper raw materials or making more valuable products. Additionally, they can be used to help shift the equilibrium of otherwise thermodynamically unfavourable reactions to give a higher conversion of the target product. By coupling an energetically unfavourable reaction with a more favourable one, the multi-enzyme cascade mimics the approach taken in nature in metabolic pathways. Nevertheless, it can be challenging to combine several engineered enzymes in vitro for the conversion of non-natural substrates. In this mini-review we focus on enzyme coupling reactions as a tool to alleviate thermodynamic constraints in synthetically useful biocatalytic reactions. The implications of thermodynamic parameters such as the equilibrium constant on the multi-enzyme cascades and the conventional methods of equilibrium shifting are also discussed in addition to methods used to estimate such values.
format	Article
author	Rohana, Abu Woodley, John M.
author_facet	Rohana, Abu Woodley, John M.
author_sort	Rohana, Abu
title	Application of Enzyme Coupling Reactions to Shift Thermodynamically Limited Biocatalytic Reactions
title_short	Application of Enzyme Coupling Reactions to Shift Thermodynamically Limited Biocatalytic Reactions
title_full	Application of Enzyme Coupling Reactions to Shift Thermodynamically Limited Biocatalytic Reactions
title_fullStr	Application of Enzyme Coupling Reactions to Shift Thermodynamically Limited Biocatalytic Reactions
title_full_unstemmed	Application of Enzyme Coupling Reactions to Shift Thermodynamically Limited Biocatalytic Reactions
title_sort	application of enzyme coupling reactions to shift thermodynamically limited biocatalytic reactions
publisher	Wiley-VCH Verlag GmbH & Co KGaA
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/11812/ http://umpir.ump.edu.my/id/eprint/11812/ http://umpir.ump.edu.my/id/eprint/11812/ http://umpir.ump.edu.my/id/eprint/11812/1/Application%20of%20Enzyme%20Coupling%20Reactions%20to%20Shift%20Thermodynamically%20Limited%20Biocatalytic%20Reactions.pdf
first_indexed	2023-09-18T22:12:47Z
last_indexed	2023-09-18T22:12:47Z
_version_	1777415139220258816

Application of Enzyme Coupling Reactions to Shift Thermodynamically Limited Biocatalytic Reactions

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