Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix

Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix

In this study, enzymatic oxidation of glycerol was performed for the production of glyceric acid. The effectiveness of immobilised laccase in Na-Alginate matrix was also verified. Glycerol was incubated at 25oC with 30 mM of 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO), which acted as a mediator, in...

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Main Authors: Chi, Shein Hong, Chin, S. Y., Cheng, C. K., Mohd Sabri, Mahmud, Chua, Gek Kee
Format: Article
Language:English
Published: Elsevier Ltd 2015
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/11773/
http://umpir.ump.edu.my/id/eprint/11773/
http://umpir.ump.edu.my/id/eprint/11773/
http://umpir.ump.edu.my/id/eprint/11773/1/Enzymatic%20Conversion%20of%20Glycerol%20to%20Glyceric%20Acid%20with%20Immobilised%20Laccase%20in%20Na-Alginate%20Matrix.pdf
id ump-11773
recordtype eprints
spelling ump-117732018-01-11T02:39:39Z http://umpir.ump.edu.my/id/eprint/11773/ Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix Chi, Shein Hong Chin, S. Y. Cheng, C. K. Mohd Sabri, Mahmud Chua, Gek Kee TP Chemical technology In this study, enzymatic oxidation of glycerol was performed for the production of glyceric acid. The effectiveness of immobilised laccase in Na-Alginate matrix was also verified. Glycerol was incubated at 25oC with 30 mM of 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO), which acted as a mediator, in the presence of immobilized laccase from Trametes versicolor. Glyceric acid was quantified using HPLC, while the activity of enzyme was measured using ABTS assay. The yield of laccase activity was 90%. On the other hand, glyceric acid obtained was around 7 wt% from the total product at 24 h. In conclusion, enzymatic oxidation is able to produce comparable amount of glyceric acid through a more environmental friendly method. Elsevier Ltd 2015 Article PeerReviewed application/pdf en cc_by_nc_nd http://umpir.ump.edu.my/id/eprint/11773/1/Enzymatic%20Conversion%20of%20Glycerol%20to%20Glyceric%20Acid%20with%20Immobilised%20Laccase%20in%20Na-Alginate%20Matrix.pdf Chi, Shein Hong and Chin, S. Y. and Cheng, C. K. and Mohd Sabri, Mahmud and Chua, Gek Kee (2015) Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix. Procedia Chemistry, 16. pp. 632-639. ISSN 1876-6196 http://dx.doi.org/10.1016/j.proche.2015.12.102 DOI: 10.1016/j.proche.2015.12.102
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Chi, Shein Hong
Chin, S. Y.
Cheng, C. K.
Mohd Sabri, Mahmud
Chua, Gek Kee
Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix
description In this study, enzymatic oxidation of glycerol was performed for the production of glyceric acid. The effectiveness of immobilised laccase in Na-Alginate matrix was also verified. Glycerol was incubated at 25oC with 30 mM of 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO), which acted as a mediator, in the presence of immobilized laccase from Trametes versicolor. Glyceric acid was quantified using HPLC, while the activity of enzyme was measured using ABTS assay. The yield of laccase activity was 90%. On the other hand, glyceric acid obtained was around 7 wt% from the total product at 24 h. In conclusion, enzymatic oxidation is able to produce comparable amount of glyceric acid through a more environmental friendly method.
format Article
author Chi, Shein Hong
Chin, S. Y.
Cheng, C. K.
Mohd Sabri, Mahmud
Chua, Gek Kee
author_facet Chi, Shein Hong
Chin, S. Y.
Cheng, C. K.
Mohd Sabri, Mahmud
Chua, Gek Kee
author_sort Chi, Shein Hong
title Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix
title_short Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix
title_full Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix
title_fullStr Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix
title_full_unstemmed Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix
title_sort enzymatic conversion of glycerol to glyceric acid with immobilised laccase in na-alginate matrix
publisher Elsevier Ltd
publishDate 2015
url http://umpir.ump.edu.my/id/eprint/11773/
http://umpir.ump.edu.my/id/eprint/11773/
http://umpir.ump.edu.my/id/eprint/11773/
http://umpir.ump.edu.my/id/eprint/11773/1/Enzymatic%20Conversion%20of%20Glycerol%20to%20Glyceric%20Acid%20with%20Immobilised%20Laccase%20in%20Na-Alginate%20Matrix.pdf
first_indexed 2023-09-18T22:12:44Z
last_indexed 2023-09-18T22:12:44Z
_version_ 1777415135309070336

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