Bioassay Guided Isolation and Identification of a Cytotoxic Compound from Azadirachta indica Leaves

This study aimed to investigate the structural features of the isolated flavonol glycoside, which might behave as a cytotoxic compound. The hexane, chloroform, ethyl acetate, and aqueous fractions of an 80% methanol solution of Neem (Azadirachta indica) (Family: Meliaceae) leaves were subjected to a...

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Main Authors:	Jessinta, Sandanasamy, Azhari, H. Nour, Hazrulrizawati, Hamid, Ahmad Ziad, Sulaiman, Saiful Nizam, Tajuddin, Nour, A. H.
Format:	Article
Language:	English
Published:	John Wiley & Sons, Inc. 2015
Subjects:	Q Science (General) TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/11331/ http://umpir.ump.edu.my/id/eprint/11331/ http://umpir.ump.edu.my/id/eprint/11331/ http://umpir.ump.edu.my/id/eprint/11331/1/Bioassay%20Guided%20Isolation%20and%20Identification%20of%20a%20Cytotoxic%20Compound%20from%20Azadirachta%20indica%20Leaves.pdf

id	ump-11331
recordtype	eprints
spelling	ump-113312018-01-24T06:45:15Z http://umpir.ump.edu.my/id/eprint/11331/ Bioassay Guided Isolation and Identification of a Cytotoxic Compound from Azadirachta indica Leaves Jessinta, Sandanasamy Azhari, H. Nour Hazrulrizawati, Hamid Ahmad Ziad, Sulaiman Saiful Nizam, Tajuddin Nour, A. H. Q Science (General) TP Chemical technology This study aimed to investigate the structural features of the isolated flavonol glycoside, which might behave as a cytotoxic compound. The hexane, chloroform, ethyl acetate, and aqueous fractions of an 80% methanol solution of Neem (Azadirachta indica) (Family: Meliaceae) leaves were subjected to a cytotoxicity bioassay against brine shrimp, Artemia salina. The ethyl acetate fraction exhibited the highest cytotoxic effect, supported by the lowest lethal concentration, a LC50 value of 1.35±0.40 ppm. A compound, Quercetin 3-O-β-D-glucopyranoside, was isolated from the most toxic fraction of the ethyl acetate via preparative liquid chromatography and then identified via ultraviolet-visible (UV-Vis), infrared (IR), mass spectrum (MS) and nuclear magnetic resonance (NMR) analyses. The compound was further confirmed by physical state, color, solubility, and melting point determination. The cytotoxic results suggest that the leaf ethyl acetate fraction consists of toxic compounds, which point towards the isolation of Quercetin 3-O-β-D-glucopyranoside. John Wiley & Sons, Inc. 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/11331/1/Bioassay%20Guided%20Isolation%20and%20Identification%20of%20a%20Cytotoxic%20Compound%20from%20Azadirachta%20indica%20Leaves.pdf Jessinta, Sandanasamy and Azhari, H. Nour and Hazrulrizawati, Hamid and Ahmad Ziad, Sulaiman and Saiful Nizam, Tajuddin and Nour, A. H. (2015) Bioassay Guided Isolation and Identification of a Cytotoxic Compound from Azadirachta indica Leaves. Journal of the Chinese Chemical Society, 62 (8). pp. 680-688. ISSN 0009-4536 (print); 2192-6549 (online) http://onlinelibrary.wiley.com/doi/10.1002/jccs.201400461/abstract;jsessionid=B9D913E989CF4B333F0E947C4C4ED82D.f04t02?userIsAuthenticated=false&deniedAccessCustomisedMessage= DOI: 10.1002/jccs.201400461
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General) TP Chemical technology
spellingShingle	Q Science (General) TP Chemical technology Jessinta, Sandanasamy Azhari, H. Nour Hazrulrizawati, Hamid Ahmad Ziad, Sulaiman Saiful Nizam, Tajuddin Nour, A. H. Bioassay Guided Isolation and Identification of a Cytotoxic Compound from Azadirachta indica Leaves
description	This study aimed to investigate the structural features of the isolated flavonol glycoside, which might behave as a cytotoxic compound. The hexane, chloroform, ethyl acetate, and aqueous fractions of an 80% methanol solution of Neem (Azadirachta indica) (Family: Meliaceae) leaves were subjected to a cytotoxicity bioassay against brine shrimp, Artemia salina. The ethyl acetate fraction exhibited the highest cytotoxic effect, supported by the lowest lethal concentration, a LC50 value of 1.35±0.40 ppm. A compound, Quercetin 3-O-β-D-glucopyranoside, was isolated from the most toxic fraction of the ethyl acetate via preparative liquid chromatography and then identified via ultraviolet-visible (UV-Vis), infrared (IR), mass spectrum (MS) and nuclear magnetic resonance (NMR) analyses. The compound was further confirmed by physical state, color, solubility, and melting point determination. The cytotoxic results suggest that the leaf ethyl acetate fraction consists of toxic compounds, which point towards the isolation of Quercetin 3-O-β-D-glucopyranoside.
format	Article
author	Jessinta, Sandanasamy Azhari, H. Nour Hazrulrizawati, Hamid Ahmad Ziad, Sulaiman Saiful Nizam, Tajuddin Nour, A. H.
author_facet	Jessinta, Sandanasamy Azhari, H. Nour Hazrulrizawati, Hamid Ahmad Ziad, Sulaiman Saiful Nizam, Tajuddin Nour, A. H.
author_sort	Jessinta, Sandanasamy
title	Bioassay Guided Isolation and Identification of a Cytotoxic Compound from Azadirachta indica Leaves
title_short	Bioassay Guided Isolation and Identification of a Cytotoxic Compound from Azadirachta indica Leaves
title_full	Bioassay Guided Isolation and Identification of a Cytotoxic Compound from Azadirachta indica Leaves
title_fullStr	Bioassay Guided Isolation and Identification of a Cytotoxic Compound from Azadirachta indica Leaves
title_full_unstemmed	Bioassay Guided Isolation and Identification of a Cytotoxic Compound from Azadirachta indica Leaves
title_sort	bioassay guided isolation and identification of a cytotoxic compound from azadirachta indica leaves
publisher	John Wiley & Sons, Inc.
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/11331/ http://umpir.ump.edu.my/id/eprint/11331/ http://umpir.ump.edu.my/id/eprint/11331/ http://umpir.ump.edu.my/id/eprint/11331/1/Bioassay%20Guided%20Isolation%20and%20Identification%20of%20a%20Cytotoxic%20Compound%20from%20Azadirachta%20indica%20Leaves.pdf
first_indexed	2023-09-18T22:11:57Z
last_indexed	2023-09-18T22:11:57Z
_version_	1777415086592229376

Bioassay Guided Isolation and Identification of a Cytotoxic Compound from Azadirachta indica Leaves

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