Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis

Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis

Monoclonal antibodies (mAbs) are unique and specific drug molecules targeting the treatment of various diseases such as arthritis, immune disorders, infectious diseases, and cancer etc. Different methods such as antibody coupled affinity chromatography, hydrophobic interaction chromatography, etc.,...

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Main Authors: Santhana, Krishnan, Zularisam, A. W., Chua, Gek Kee, Chew, Few Ne, Mimi Sakinah, A. M.
Format: Article
Language:English
Published: Hibiscus Publisher 2015
Subjects:
T Technology (General)
Online Access:http://umpir.ump.edu.my/id/eprint/11150/
http://umpir.ump.edu.my/id/eprint/11150/
http://umpir.ump.edu.my/id/eprint/11150/1/Optimization%20of%20Monoclonal%20Antibodies%20Purification%20Expressed%20in%20H-192%20cells%20using%20Preparative%20Native-Polyacrylamide%20Gel%20Electrophoresis.pdf
id ump-11150
recordtype eprints
spelling ump-111502018-01-17T02:29:50Z http://umpir.ump.edu.my/id/eprint/11150/ Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis Santhana, Krishnan Zularisam, A. W. Chua, Gek Kee Chew, Few Ne Mimi Sakinah, A. M. T Technology (General) Monoclonal antibodies (mAbs) are unique and specific drug molecules targeting the treatment of various diseases such as arthritis, immune disorders, infectious diseases, and cancer etc. Different methods such as antibody coupled affinity chromatography, hydrophobic interaction chromatography, etc., can be applied to purify mAbs from various sources. This article provides a simple, cost effective, preparative native-polyacrylamide gel electrophoresis (n-PAGE) technique to purify mAbs expressed in H-192 cells (Hybridoma murine cell lines) against an antigen i.e. 17-alpha-hydroxyprogesterone (17-OHP), which further can have diagnostic application to detect Congenital Adrenal Hyperplasia (CAH). Furthermore, different parameters such as concentration and volume of the feedstock (medium containing antibodies), pore size of gel, height of resolving gel etc. were optimized to obtain the maximum purity and yield of mAbs. Hibiscus Publisher 2015 Article PeerReviewed application/pdf en cc_by http://umpir.ump.edu.my/id/eprint/11150/1/Optimization%20of%20Monoclonal%20Antibodies%20Purification%20Expressed%20in%20H-192%20cells%20using%20Preparative%20Native-Polyacrylamide%20Gel%20Electrophoresis.pdf Santhana, Krishnan and Zularisam, A. W. and Chua, Gek Kee and Chew, Few Ne and Mimi Sakinah, A. M. (2015) Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis. Journal Of Biochemistry, Microbiology and Biotechnology , 3 (1). pp. 21-25. ISSN 2289-5779 http://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/231
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic T Technology (General)
spellingShingle T Technology (General)
Santhana, Krishnan
Zularisam, A. W.
Chua, Gek Kee
Chew, Few Ne
Mimi Sakinah, A. M.
Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis
description Monoclonal antibodies (mAbs) are unique and specific drug molecules targeting the treatment of various diseases such as arthritis, immune disorders, infectious diseases, and cancer etc. Different methods such as antibody coupled affinity chromatography, hydrophobic interaction chromatography, etc., can be applied to purify mAbs from various sources. This article provides a simple, cost effective, preparative native-polyacrylamide gel electrophoresis (n-PAGE) technique to purify mAbs expressed in H-192 cells (Hybridoma murine cell lines) against an antigen i.e. 17-alpha-hydroxyprogesterone (17-OHP), which further can have diagnostic application to detect Congenital Adrenal Hyperplasia (CAH). Furthermore, different parameters such as concentration and volume of the feedstock (medium containing antibodies), pore size of gel, height of resolving gel etc. were optimized to obtain the maximum purity and yield of mAbs.
format Article
author Santhana, Krishnan
Zularisam, A. W.
Chua, Gek Kee
Chew, Few Ne
Mimi Sakinah, A. M.
author_facet Santhana, Krishnan
Zularisam, A. W.
Chua, Gek Kee
Chew, Few Ne
Mimi Sakinah, A. M.
author_sort Santhana, Krishnan
title Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis
title_short Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis
title_full Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis
title_fullStr Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis
title_full_unstemmed Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis
title_sort optimization of monoclonal antibodies purification expressed in h-192 cells using preparative native-polyacrylamide gel electrophoresis
publisher Hibiscus Publisher
publishDate 2015
url http://umpir.ump.edu.my/id/eprint/11150/
http://umpir.ump.edu.my/id/eprint/11150/
http://umpir.ump.edu.my/id/eprint/11150/1/Optimization%20of%20Monoclonal%20Antibodies%20Purification%20Expressed%20in%20H-192%20cells%20using%20Preparative%20Native-Polyacrylamide%20Gel%20Electrophoresis.pdf
first_indexed 2023-09-18T22:11:36Z
last_indexed 2023-09-18T22:11:36Z
_version_ 1777415064888803328

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