Initial screening of mangrove endophytic fungi for antimicrobial compounds and heavy metal biosorption potential

Endophytic fungi provide protection to their host plant and the fungi often produce antimicrobial compounds to aid the host fighting off pathogens. These bioactive compounds were secondary metabolites which were often produced as waste- or by-products. In the present study, endophytic fungi isolated...

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Main Authors:	Onn, M. Ling, Lim, P. Teen, Aazani Mujahid, Proksch, Peter, Müller, Moritz
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2016
Online Access:	http://journalarticle.ukm.my/9980/ http://journalarticle.ukm.my/9980/ http://journalarticle.ukm.my/9980/1/08%20Onn%20M.%20Ling.pdf

id	ukm-9980
recordtype	eprints
spelling	ukm-99802017-01-19T09:24:32Z http://journalarticle.ukm.my/9980/ Initial screening of mangrove endophytic fungi for antimicrobial compounds and heavy metal biosorption potential Onn, M. Ling Lim, P. Teen Aazani Mujahid, Proksch, Peter Müller, Moritz Endophytic fungi provide protection to their host plant and the fungi often produce antimicrobial compounds to aid the host fighting off pathogens. These bioactive compounds were secondary metabolites which were often produced as waste- or by-products. In the present study, endophytic fungi isolated from mangrove plants and soils were characterized and their antimicrobial production and bioremediation potential of heavy metals copper (Cu) and zinc (Zn) were assessed. Twelve (12) isolated and identified endophytic fungi belonged to seven species; Penicillium, Curvularia, Diaporthe, Aspergillus, Guignardia, Neusartorya and Eupenicillium. Antimicrobial activities of these 12 fungal endophytes were tested against Gram negative bacteria; Bacillus subtilis, Staphylococcus aureus, Gram positive bacteria; Escherichia coli and fungi; Candida albicans and Aspergillus niger among others. Two isolates (related to Guignardia sp. and Neusartoya sp.) showed strong antimicrobial (and antifungal) activity whereas the rest showed no activity. Compounds were isolated from both isolates and screened using HPLC. Both isolates displayed chemically very interesting chromatograms as they possessed a high diversity of basic chemical structures and peaks over a wide range of polarities, with structures similar to Trimeric catechin and Helenalin among others. For bioremediation assessment, the results showed maximum biosorption capacity for two isolates related to Curvularia sp. and Neusartorya sp., with the former removing 25 mg Cu/g biomass and the latter removing 24 mg Zn/g biomass. Our results indicated the potential of mangrove endophytic fungi in producing bioactive compounds and also highlighted their potential for the treatment of heavy metal-contaminated wastewater. Penerbit Universiti Kebangsaan Malaysia 2016-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9980/1/08%20Onn%20M.%20Ling.pdf Onn, M. Ling and Lim, P. Teen and Aazani Mujahid, and Proksch, Peter and Müller, Moritz (2016) Initial screening of mangrove endophytic fungi for antimicrobial compounds and heavy metal biosorption potential. Sains Malaysiana, 45 (7). pp. 1063-1071. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol45num7_2016/contentsVol45num7_2016.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Endophytic fungi provide protection to their host plant and the fungi often produce antimicrobial compounds to aid the host fighting off pathogens. These bioactive compounds were secondary metabolites which were often produced as waste- or by-products. In the present study, endophytic fungi isolated from mangrove plants and soils were characterized and their antimicrobial production and bioremediation potential of heavy metals copper (Cu) and zinc (Zn) were assessed. Twelve (12) isolated and identified endophytic fungi belonged to seven species; Penicillium, Curvularia, Diaporthe, Aspergillus, Guignardia, Neusartorya and Eupenicillium. Antimicrobial activities of these 12 fungal endophytes were tested against Gram negative bacteria; Bacillus subtilis, Staphylococcus aureus, Gram positive bacteria; Escherichia coli and fungi; Candida albicans and Aspergillus niger among others. Two isolates (related to Guignardia sp. and Neusartoya sp.) showed strong antimicrobial (and antifungal) activity whereas the rest showed no activity. Compounds were isolated from both isolates and screened using HPLC. Both isolates displayed chemically very interesting chromatograms as they possessed a high diversity of basic chemical structures and peaks over a wide range of polarities, with structures similar to Trimeric catechin and Helenalin among others. For bioremediation assessment, the results showed maximum biosorption capacity for two isolates related to Curvularia sp. and Neusartorya sp., with the former removing 25 mg Cu/g biomass and the latter removing 24 mg Zn/g biomass. Our results indicated the potential of mangrove endophytic fungi in producing bioactive compounds and also highlighted their potential for the treatment of heavy metal-contaminated wastewater.
format	Article
author	Onn, M. Ling Lim, P. Teen Aazani Mujahid, Proksch, Peter Müller, Moritz
spellingShingle	Onn, M. Ling Lim, P. Teen Aazani Mujahid, Proksch, Peter Müller, Moritz Initial screening of mangrove endophytic fungi for antimicrobial compounds and heavy metal biosorption potential
author_facet	Onn, M. Ling Lim, P. Teen Aazani Mujahid, Proksch, Peter Müller, Moritz
author_sort	Onn, M. Ling
title	Initial screening of mangrove endophytic fungi for antimicrobial compounds and heavy metal biosorption potential
title_short	Initial screening of mangrove endophytic fungi for antimicrobial compounds and heavy metal biosorption potential
title_full	Initial screening of mangrove endophytic fungi for antimicrobial compounds and heavy metal biosorption potential
title_fullStr	Initial screening of mangrove endophytic fungi for antimicrobial compounds and heavy metal biosorption potential
title_full_unstemmed	Initial screening of mangrove endophytic fungi for antimicrobial compounds and heavy metal biosorption potential
title_sort	initial screening of mangrove endophytic fungi for antimicrobial compounds and heavy metal biosorption potential
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2016
url	http://journalarticle.ukm.my/9980/ http://journalarticle.ukm.my/9980/ http://journalarticle.ukm.my/9980/1/08%20Onn%20M.%20Ling.pdf
first_indexed	2023-09-18T19:56:16Z
last_indexed	2023-09-18T19:56:16Z
_version_	1777406550489432064

Initial screening of mangrove endophytic fungi for antimicrobial compounds and heavy metal biosorption potential

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