Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production

Large numbers of glycerol-consuming bacteria are present in nature; hence bioconversion of glycerol into biofuel which is bioethanol is one of the interests. The effective screening procedure is needed to screen and isolate broad ranges of bacteria from environment. The screening method was modified...

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Main Authors:	Sheril Norliana Suhaimi, Nur Amelia Azreen Adnan, Yee, Phang Lai, Suraini Abd-Aziz, Maeda, Toshinari
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2016
Online Access:	http://journalarticle.ukm.my/9886/ http://journalarticle.ukm.my/9886/ http://journalarticle.ukm.my/9886/1/18_Sheril_Norliana_Suhaimi.pdf

id	ukm-9886
recordtype	eprints
spelling	ukm-98862016-12-14T06:51:09Z http://journalarticle.ukm.my/9886/ Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production Sheril Norliana Suhaimi, Nur Amelia Azreen Adnan, Yee, Phang Lai Suraini Abd-Aziz, Maeda, Toshinari Large numbers of glycerol-consuming bacteria are present in nature; hence bioconversion of glycerol into biofuel which is bioethanol is one of the interests. The effective screening procedure is needed to screen and isolate broad ranges of bacteria from environment. The screening method was modified based on enzymatic oxidation of ethanol, which is correlated to reduction of 2,6-dichlorophenol-indophenol dye that resulted in the formation of yellow zone. Approximately 300 colonies were able to grow on minimal media using glycerol as sole carbon. Only about 70 isolates showed positive result when using the modified ethanol production assay after pre-screening stage. The formation of decolourized zone was apparent using modified assay containing 5 mL/L of 0.05M 2,6-dichlorophenol-indophenol, 10 mL of reaction mixture and 500 μl/L of enzyme, respectively. The ethanol production capability of the isolates was further proven by anaerobic fermentation as a quantitative method. This modified method is applicable in screening for ethanol producer from glycerol as carbon source allows rapid and more bacteria can be screened. Penerbit Universiti Kebangsaan Malaysia 2016-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9886/1/18_Sheril_Norliana_Suhaimi.pdf Sheril Norliana Suhaimi, and Nur Amelia Azreen Adnan, and Yee, Phang Lai and Suraini Abd-Aziz, and Maeda, Toshinari (2016) Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production. Sains Malaysiana, 45 (5). pp. 811-815. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol45num5_2016/contentsVol45num5_2016.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Large numbers of glycerol-consuming bacteria are present in nature; hence bioconversion of glycerol into biofuel which is bioethanol is one of the interests. The effective screening procedure is needed to screen and isolate broad ranges of bacteria from environment. The screening method was modified based on enzymatic oxidation of ethanol, which is correlated to reduction of 2,6-dichlorophenol-indophenol dye that resulted in the formation of yellow zone. Approximately 300 colonies were able to grow on minimal media using glycerol as sole carbon. Only about 70 isolates showed positive result when using the modified ethanol production assay after pre-screening stage. The formation of decolourized zone was apparent using modified assay containing 5 mL/L of 0.05M 2,6-dichlorophenol-indophenol, 10 mL of reaction mixture and 500 μl/L of enzyme, respectively. The ethanol production capability of the isolates was further proven by anaerobic fermentation as a quantitative method. This modified method is applicable in screening for ethanol producer from glycerol as carbon source allows rapid and more bacteria can be screened.
format	Article
author	Sheril Norliana Suhaimi, Nur Amelia Azreen Adnan, Yee, Phang Lai Suraini Abd-Aziz, Maeda, Toshinari
spellingShingle	Sheril Norliana Suhaimi, Nur Amelia Azreen Adnan, Yee, Phang Lai Suraini Abd-Aziz, Maeda, Toshinari Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
author_facet	Sheril Norliana Suhaimi, Nur Amelia Azreen Adnan, Yee, Phang Lai Suraini Abd-Aziz, Maeda, Toshinari
author_sort	Sheril Norliana Suhaimi,
title	Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
title_short	Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
title_full	Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
title_fullStr	Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
title_full_unstemmed	Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
title_sort	rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2016
url	http://journalarticle.ukm.my/9886/ http://journalarticle.ukm.my/9886/ http://journalarticle.ukm.my/9886/1/18_Sheril_Norliana_Suhaimi.pdf
first_indexed	2023-09-18T19:56:00Z
last_indexed	2023-09-18T19:56:00Z
_version_	1777406532847140864

Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production

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