A medicinal ginger, boesenbergia rotunda: from cell suspension cultures to protoplast derived callus

Boesenbergia rotunda is a medicinal ginger that has been found to contain several bioactive compounds such as boesenbergin A, panduratin A, cardamonin, pinostrobin and pinocembrin. These compounds are useful in treating various ailments, such as oral diseases, inflammation and have also been used as...

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Main Authors:	Tan, Hao-Cheak, Tan, Boon-Chin, Wong, Sher-Ming, Norzulaani Khalid
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2016
Online Access:	http://journalarticle.ukm.my/9884/ http://journalarticle.ukm.my/9884/ http://journalarticle.ukm.my/9884/1/16_Hao-Cheak_Tan.pdf

id	ukm-9884
recordtype	eprints
spelling	ukm-98842016-12-14T06:51:08Z http://journalarticle.ukm.my/9884/ A medicinal ginger, boesenbergia rotunda: from cell suspension cultures to protoplast derived callus Tan, Hao-Cheak Tan, Boon-Chin Wong, Sher-Ming Norzulaani Khalid, Boesenbergia rotunda is a medicinal ginger that has been found to contain several bioactive compounds such as boesenbergin A, panduratin A, cardamonin, pinostrobin and pinocembrin. These compounds are useful in treating various ailments, such as oral diseases, inflammation and have also been used as an aphrodisiac. In this study, an efficient protocol for developing and isolating protoplast cultures for B. rotunda has been established. Rhizome buds of B. rotunda were used as explants to initiate callus growth and the established cell suspension cultures were used to optimize their growth conditions. Our results indicated that embryogenic suspension cultures in liquid Murashige and Skoog (MS) medium supplemented with 3% (w/v) sucrose produced the highest growth rate (μ = 0.1125), whereas no promotive effect was seen in the presence of 2,4-dichlorophenoxyacetic acid and those that underwent sonication treatment. Amount of protoplasts isolated ranging from 1-5 × 105 protoplast per mL were isolated using 0.25% (w/v) macerozyme and 1% (w/v) cellulase for 24 h under continuous agitation (50 rpm) in dark condition. Of the isolated protoplasts, 54.93% were viable according to fluorescein diacetate staining test. Micro-colonies were recovered in liquid MS medium containing 9 g/L mannitol, 2 mg/L 1-naphthaleneacetic acid and 0.5 mg/L benzylaminopurine (BAP) for 4 weeks and subsequently transferred to solid MS medium supplemented with 0.5 mg/L BAP for callus initiation. The protoplast system established in this study would be useful for genetic manipulation and modern breeding program of B. rotunda. Penerbit Universiti Kebangsaan Malaysia 2016-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9884/1/16_Hao-Cheak_Tan.pdf Tan, Hao-Cheak and Tan, Boon-Chin and Wong, Sher-Ming and Norzulaani Khalid, (2016) A medicinal ginger, boesenbergia rotunda: from cell suspension cultures to protoplast derived callus. Sains Malaysiana, 45 (5). pp. 795-802. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol45num5_2016/contentsVol45num5_2016.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Boesenbergia rotunda is a medicinal ginger that has been found to contain several bioactive compounds such as boesenbergin A, panduratin A, cardamonin, pinostrobin and pinocembrin. These compounds are useful in treating various ailments, such as oral diseases, inflammation and have also been used as an aphrodisiac. In this study, an efficient protocol for developing and isolating protoplast cultures for B. rotunda has been established. Rhizome buds of B. rotunda were used as explants to initiate callus growth and the established cell suspension cultures were used to optimize their growth conditions. Our results indicated that embryogenic suspension cultures in liquid Murashige and Skoog (MS) medium supplemented with 3% (w/v) sucrose produced the highest growth rate (μ = 0.1125), whereas no promotive effect was seen in the presence of 2,4-dichlorophenoxyacetic acid and those that underwent sonication treatment. Amount of protoplasts isolated ranging from 1-5 × 105 protoplast per mL were isolated using 0.25% (w/v) macerozyme and 1% (w/v) cellulase for 24 h under continuous agitation (50 rpm) in dark condition. Of the isolated protoplasts, 54.93% were viable according to fluorescein diacetate staining test. Micro-colonies were recovered in liquid MS medium containing 9 g/L mannitol, 2 mg/L 1-naphthaleneacetic acid and 0.5 mg/L benzylaminopurine (BAP) for 4 weeks and subsequently transferred to solid MS medium supplemented with 0.5 mg/L BAP for callus initiation. The protoplast system established in this study would be useful for genetic manipulation and modern breeding program of B. rotunda.
format	Article
author	Tan, Hao-Cheak Tan, Boon-Chin Wong, Sher-Ming Norzulaani Khalid,
spellingShingle	Tan, Hao-Cheak Tan, Boon-Chin Wong, Sher-Ming Norzulaani Khalid, A medicinal ginger, boesenbergia rotunda: from cell suspension cultures to protoplast derived callus
author_facet	Tan, Hao-Cheak Tan, Boon-Chin Wong, Sher-Ming Norzulaani Khalid,
author_sort	Tan, Hao-Cheak
title	A medicinal ginger, boesenbergia rotunda: from cell suspension cultures to protoplast derived callus
title_short	A medicinal ginger, boesenbergia rotunda: from cell suspension cultures to protoplast derived callus
title_full	A medicinal ginger, boesenbergia rotunda: from cell suspension cultures to protoplast derived callus
title_fullStr	A medicinal ginger, boesenbergia rotunda: from cell suspension cultures to protoplast derived callus
title_full_unstemmed	A medicinal ginger, boesenbergia rotunda: from cell suspension cultures to protoplast derived callus
title_sort	medicinal ginger, boesenbergia rotunda: from cell suspension cultures to protoplast derived callus
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2016
url	http://journalarticle.ukm.my/9884/ http://journalarticle.ukm.my/9884/ http://journalarticle.ukm.my/9884/1/16_Hao-Cheak_Tan.pdf
first_indexed	2023-09-18T19:55:59Z
last_indexed	2023-09-18T19:55:59Z
_version_	1777406532545150976

A medicinal ginger, boesenbergia rotunda: from cell suspension cultures to protoplast derived callus

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