Replication of a Malaysian Strain Avian Influenza A Virus H5N1 in Madin-Darby Canine Kidney and African Green Monkey Kidney cells

Replication of a Malaysian Strain Avian Influenza A Virus H5N1 in Madin-Darby Canine Kidney and African Green Monkey Kidney cells

The use of cell lines such as Madin-Darby Canine Kidney (MDCK) and African Green Monkey Kidney (Vero) cells in influenza vaccine production is much advocated presently as a safer alternative to chicken embryonated eggs. It is thus essential to understand the influenza virus replication patterns in t...

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Main Authors: Tan, Toong Seng, Sharifah Syed Hassan, Yap, Wei Boon
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2016
Online Access:http://journalarticle.ukm.my/9883/
http://journalarticle.ukm.my/9883/
http://journalarticle.ukm.my/9883/1/15_Toong_Seng_Tan.pdf
id ukm-9883
recordtype eprints
spelling ukm-98832016-12-14T06:51:08Z http://journalarticle.ukm.my/9883/ Replication of a Malaysian Strain Avian Influenza A Virus H5N1 in Madin-Darby Canine Kidney and African Green Monkey Kidney cells Tan, Toong Seng Sharifah Syed Hassan, Yap, Wei Boon The use of cell lines such as Madin-Darby Canine Kidney (MDCK) and African Green Monkey Kidney (Vero) cells in influenza vaccine production is much advocated presently as a safer alternative to chicken embryonated eggs. It is thus essential to understand the influenza virus replication patterns in these cell lines prior to utilizing them in vaccine production. The infectivity of avian influenza A virus (A/Chicken/Malaysia/5858/2004) H5N1 in MDCK and Vero cell lines was first assessed by comparing the cytopathic effect (CPE) caused by the virus infection. The viral loads in both of the infected media and cells were also compared. The results showed that both of the MDCK and Vero cells began to exhibit significant CPE (p<0.05) after 48 h post-infection (h p.i). The MDCK cell line was more susceptible to the virus infection compared to Vero cell line throughout the incubation period. A higher viral load was also detected in the host cells compared to their respective culturing media. Interestingly, after reaching its maximum titer at 48 h p.i, the viral load in MDCK cells declined meanwhile the viral load in Vero cells increased gradually and peaked at 120 h p.i. Overall, both cell lines support efficient H5N1 virus replication. While the peak viral loads measured in the two cell lines did not differ much, a more rapid replication was observed in the infected MDCK samples. The finding showed that MDCK cell line might serve as a more time-saving and cost-effective cell culture-based system compared to Vero cell line for influenza vaccine production. Penerbit Universiti Kebangsaan Malaysia 2016-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9883/1/15_Toong_Seng_Tan.pdf Tan, Toong Seng and Sharifah Syed Hassan, and Yap, Wei Boon (2016) Replication of a Malaysian Strain Avian Influenza A Virus H5N1 in Madin-Darby Canine Kidney and African Green Monkey Kidney cells. Sains Malaysiana, 45 (5). pp. 787-793. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol45num5_2016/contentsVol45num5_2016.html
repository_type Digital Repository
institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description The use of cell lines such as Madin-Darby Canine Kidney (MDCK) and African Green Monkey Kidney (Vero) cells in influenza vaccine production is much advocated presently as a safer alternative to chicken embryonated eggs. It is thus essential to understand the influenza virus replication patterns in these cell lines prior to utilizing them in vaccine production. The infectivity of avian influenza A virus (A/Chicken/Malaysia/5858/2004) H5N1 in MDCK and Vero cell lines was first assessed by comparing the cytopathic effect (CPE) caused by the virus infection. The viral loads in both of the infected media and cells were also compared. The results showed that both of the MDCK and Vero cells began to exhibit significant CPE (p<0.05) after 48 h post-infection (h p.i). The MDCK cell line was more susceptible to the virus infection compared to Vero cell line throughout the incubation period. A higher viral load was also detected in the host cells compared to their respective culturing media. Interestingly, after reaching its maximum titer at 48 h p.i, the viral load in MDCK cells declined meanwhile the viral load in Vero cells increased gradually and peaked at 120 h p.i. Overall, both cell lines support efficient H5N1 virus replication. While the peak viral loads measured in the two cell lines did not differ much, a more rapid replication was observed in the infected MDCK samples. The finding showed that MDCK cell line might serve as a more time-saving and cost-effective cell culture-based system compared to Vero cell line for influenza vaccine production.
format Article
author Tan, Toong Seng
Sharifah Syed Hassan,
Yap, Wei Boon
spellingShingle Tan, Toong Seng
Sharifah Syed Hassan,
Yap, Wei Boon
Replication of a Malaysian Strain Avian Influenza A Virus H5N1 in Madin-Darby Canine Kidney and African Green Monkey Kidney cells
author_facet Tan, Toong Seng
Sharifah Syed Hassan,
Yap, Wei Boon
author_sort Tan, Toong Seng
title Replication of a Malaysian Strain Avian Influenza A Virus H5N1 in Madin-Darby Canine Kidney and African Green Monkey Kidney cells
title_short Replication of a Malaysian Strain Avian Influenza A Virus H5N1 in Madin-Darby Canine Kidney and African Green Monkey Kidney cells
title_full Replication of a Malaysian Strain Avian Influenza A Virus H5N1 in Madin-Darby Canine Kidney and African Green Monkey Kidney cells
title_fullStr Replication of a Malaysian Strain Avian Influenza A Virus H5N1 in Madin-Darby Canine Kidney and African Green Monkey Kidney cells
title_full_unstemmed Replication of a Malaysian Strain Avian Influenza A Virus H5N1 in Madin-Darby Canine Kidney and African Green Monkey Kidney cells
title_sort replication of a malaysian strain avian influenza a virus h5n1 in madin-darby canine kidney and african green monkey kidney cells
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2016
url http://journalarticle.ukm.my/9883/
http://journalarticle.ukm.my/9883/
http://journalarticle.ukm.my/9883/1/15_Toong_Seng_Tan.pdf
first_indexed 2023-09-18T19:55:59Z
last_indexed 2023-09-18T19:55:59Z
_version_ 1777406532396253184

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