Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede

The objective of this study was to determine the Immunoglobulin E-binding proteins (IgE) and major allergens of Scomberomorus commerson Lacepede(Narrow-barred Spanish mackerel). Allergen extracts were obtained from uncooked and cooked fish by homogenization in phosphate-buffered saline followed by c...

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Main Authors:	Rosmilah Misnan, Shahnaz Murad, Masita Arip, Noormalin Abdullah, Jamaludin Mohamed
Format:	Article
Language:	English
Published:	penerbit ukm 2005
Online Access:	http://journalarticle.ukm.my/983/ http://journalarticle.ukm.my/983/ http://journalarticle.ukm.my/983/1/jurnal36.pdf

id	ukm-983
recordtype	eprints
spelling	ukm-9832016-12-14T06:28:29Z http://journalarticle.ukm.my/983/ Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede Rosmilah Misnan, Shahnaz Murad, Masita Arip, Noormalin Abdullah, Jamaludin Mohamed, The objective of this study was to determine the Immunoglobulin E-binding proteins (IgE) and major allergens of Scomberomorus commerson Lacepede(Narrow-barred Spanish mackerel). Allergen extracts were obtained from uncooked and cooked fish by homogenization in phosphate-buffered saline followed by continuous extraction at 4oC or on ice. Protein profiles and IgEbinding patterns were then detected by means of sodium dodecyl polyacrylamidegel electrophoresis (SDS-PAGE) and immunoblotting using sera from patients sensitized to the fish. SDS-PAGE of the uncooked fish extracts revealed 26 protein bands in the range of about 11 to >175 kD, while the cooked extracts produced fewer protein bands. Immunoblotting demonstrated 17 IgE-binding bands, ranging in molecular weight from 11 to 151 kD. Two components with molecular weight of about ~50 and 42 kD showed the highest frequency of IgE-binding (62.2 and 51.4% respectively) and were identified as the major allergens of this fish allergy. Other IgE-binding proteins including a protein at ~12 kD which was equivalent in size to parvalbumin were identified as the minor allergens. penerbit ukm 2005 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/983/1/jurnal36.pdf Rosmilah Misnan, and Shahnaz Murad, and Masita Arip, and Noormalin Abdullah, and Jamaludin Mohamed, (2005) Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede. Jurnal Sains Kesihatan Malaysia, 3 (2). pp. 79-87. ISSN 1675-8161 http://www.fskb.ukm.my/fskbold/jurnal
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The objective of this study was to determine the Immunoglobulin E-binding proteins (IgE) and major allergens of Scomberomorus commerson Lacepede(Narrow-barred Spanish mackerel). Allergen extracts were obtained from uncooked and cooked fish by homogenization in phosphate-buffered saline followed by continuous extraction at 4oC or on ice. Protein profiles and IgEbinding patterns were then detected by means of sodium dodecyl polyacrylamidegel electrophoresis (SDS-PAGE) and immunoblotting using sera from patients sensitized to the fish. SDS-PAGE of the uncooked fish extracts revealed 26 protein bands in the range of about 11 to >175 kD, while the cooked extracts produced fewer protein bands. Immunoblotting demonstrated 17 IgE-binding bands, ranging in molecular weight from 11 to 151 kD. Two components with molecular weight of about ~50 and 42 kD showed the highest frequency of IgE-binding (62.2 and 51.4% respectively) and were identified as the major allergens of this fish allergy. Other IgE-binding proteins including a protein at ~12 kD which was equivalent in size to parvalbumin were identified as the minor allergens.
format	Article
author	Rosmilah Misnan, Shahnaz Murad, Masita Arip, Noormalin Abdullah, Jamaludin Mohamed,
spellingShingle	Rosmilah Misnan, Shahnaz Murad, Masita Arip, Noormalin Abdullah, Jamaludin Mohamed, Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede
author_facet	Rosmilah Misnan, Shahnaz Murad, Masita Arip, Noormalin Abdullah, Jamaludin Mohamed,
author_sort	Rosmilah Misnan,
title	Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede
title_short	Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede
title_full	Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede
title_fullStr	Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede
title_full_unstemmed	Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede
title_sort	characterization of immunoglobulin e-binding proteins (ige) of scomberomorus commerson lacepede
publisher	penerbit ukm
publishDate	2005
url	http://journalarticle.ukm.my/983/ http://journalarticle.ukm.my/983/ http://journalarticle.ukm.my/983/1/jurnal36.pdf
first_indexed	2023-09-18T19:32:03Z
last_indexed	2023-09-18T19:32:03Z
_version_	1777405026769043456

Characterization of immunoglobulin E-binding proteins (IgE) of scomberomorus commerson lacepede

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