Developmental potential of mouse single blastomere derived from isolated 2-, 4- and 8- cell embryos into blastocyst and Inner Cell Mass (ICM) uutgrowths

Developmental potential of mouse single blastomere derived from isolated 2-, 4- and 8- cell embryos into blastocyst and Inner Cell Mass (ICM) uutgrowths

The application of blastomere separation (BS) technique to produce embryonic stem cell (ESC) from inner cell mass (ICM) is not yet perfected due to low cleavage efficiency of single blastomere. Thefore, the aims of this study were to evaluate the developmental potential of single blastomere (SB) der...

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Main Authors: Asdiana Amri, Ramli Abdullah, Wan Khadijah Wan Embong
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2016
Online Access:http://journalarticle.ukm.my/9746/
http://journalarticle.ukm.my/9746/
http://journalarticle.ukm.my/9746/1/09_Asdiana_Amri.pdf
id ukm-9746
recordtype eprints
spelling ukm-97462016-12-14T06:50:43Z http://journalarticle.ukm.my/9746/ Developmental potential of mouse single blastomere derived from isolated 2-, 4- and 8- cell embryos into blastocyst and Inner Cell Mass (ICM) uutgrowths Asdiana Amri, Ramli Abdullah, Wan Khadijah Wan Embong, The application of blastomere separation (BS) technique to produce embryonic stem cell (ESC) from inner cell mass (ICM) is not yet perfected due to low cleavage efficiency of single blastomere. Thefore, the aims of this study were to evaluate the developmental potential of single blastomere (SB) derived from embryos at different stages to blastocyst and the potential of ICM outgrowth. The female mice of ICR strain (age: 8-12 weeks; n=47) were superovulated using gonadotrophins and mated with ICR strain males (age: 10-14 weeks; n=20). The 2-cell embryos (n=366) were flushed from the oviduct of the treated females and cultured in vitro before assigned into the following groups: a) 2-cell, b) 4-cell, c) 8-cell and d) control prior blastomere separation. The SBs were cultured in vitro and daily observations were conducted to record the cleavage rates up to Day 5. Developmental rate of 2-cell derived SB (77.28±6.77) was greater than 4-cell (63.70±5.35) and 8-cell (55.73±3.35), corresponding to the results of ICM outgrowth at 2-cell (69.29±4.13), gave higher rate followed by 4-cell (55.73±7.81) and 8-cell (41.85±3.58). Diameter of blastocyst decreased as the SB parent embryo stage increased, with the respective ratio of 5 (diameter of SB blastocyst): 3 (diameter of SB) (2-cell: 92.55±1.59 vs. 56.48±0.40; 4-cell: 78.71±1.37 vs. 44.02±0.49 and 8-cell: 64.13±2.20 vs. 35.68±0.34) as well as total cell number in blastocyst (2-cell: 43.00±1.48; 4-cell: 28.33±1.15; 8-cell: 8.80±0.58). In conclusion, SB at different stages of mouse embryos successfully develop to blastocysts in vitro that can be used as ICM source, which is a prerequisite for establishment of ESC outgrowth. Penerbit Universiti Kebangsaan Malaysia 2016-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9746/1/09_Asdiana_Amri.pdf Asdiana Amri, and Ramli Abdullah, and Wan Khadijah Wan Embong, (2016) Developmental potential of mouse single blastomere derived from isolated 2-, 4- and 8- cell embryos into blastocyst and Inner Cell Mass (ICM) uutgrowths. Sains Malaysiana, 45 (4). pp. 575-580. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid45bil4_2016/KandunganJilid45Bil4_2016.htm
repository_type Digital Repository
institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description The application of blastomere separation (BS) technique to produce embryonic stem cell (ESC) from inner cell mass (ICM) is not yet perfected due to low cleavage efficiency of single blastomere. Thefore, the aims of this study were to evaluate the developmental potential of single blastomere (SB) derived from embryos at different stages to blastocyst and the potential of ICM outgrowth. The female mice of ICR strain (age: 8-12 weeks; n=47) were superovulated using gonadotrophins and mated with ICR strain males (age: 10-14 weeks; n=20). The 2-cell embryos (n=366) were flushed from the oviduct of the treated females and cultured in vitro before assigned into the following groups: a) 2-cell, b) 4-cell, c) 8-cell and d) control prior blastomere separation. The SBs were cultured in vitro and daily observations were conducted to record the cleavage rates up to Day 5. Developmental rate of 2-cell derived SB (77.28±6.77) was greater than 4-cell (63.70±5.35) and 8-cell (55.73±3.35), corresponding to the results of ICM outgrowth at 2-cell (69.29±4.13), gave higher rate followed by 4-cell (55.73±7.81) and 8-cell (41.85±3.58). Diameter of blastocyst decreased as the SB parent embryo stage increased, with the respective ratio of 5 (diameter of SB blastocyst): 3 (diameter of SB) (2-cell: 92.55±1.59 vs. 56.48±0.40; 4-cell: 78.71±1.37 vs. 44.02±0.49 and 8-cell: 64.13±2.20 vs. 35.68±0.34) as well as total cell number in blastocyst (2-cell: 43.00±1.48; 4-cell: 28.33±1.15; 8-cell: 8.80±0.58). In conclusion, SB at different stages of mouse embryos successfully develop to blastocysts in vitro that can be used as ICM source, which is a prerequisite for establishment of ESC outgrowth.
format Article
author Asdiana Amri,
Ramli Abdullah,
Wan Khadijah Wan Embong,
spellingShingle Asdiana Amri,
Ramli Abdullah,
Wan Khadijah Wan Embong,
Developmental potential of mouse single blastomere derived from isolated 2-, 4- and 8- cell embryos into blastocyst and Inner Cell Mass (ICM) uutgrowths
author_facet Asdiana Amri,
Ramli Abdullah,
Wan Khadijah Wan Embong,
author_sort Asdiana Amri,
title Developmental potential of mouse single blastomere derived from isolated 2-, 4- and 8- cell embryos into blastocyst and Inner Cell Mass (ICM) uutgrowths
title_short Developmental potential of mouse single blastomere derived from isolated 2-, 4- and 8- cell embryos into blastocyst and Inner Cell Mass (ICM) uutgrowths
title_full Developmental potential of mouse single blastomere derived from isolated 2-, 4- and 8- cell embryos into blastocyst and Inner Cell Mass (ICM) uutgrowths
title_fullStr Developmental potential of mouse single blastomere derived from isolated 2-, 4- and 8- cell embryos into blastocyst and Inner Cell Mass (ICM) uutgrowths
title_full_unstemmed Developmental potential of mouse single blastomere derived from isolated 2-, 4- and 8- cell embryos into blastocyst and Inner Cell Mass (ICM) uutgrowths
title_sort developmental potential of mouse single blastomere derived from isolated 2-, 4- and 8- cell embryos into blastocyst and inner cell mass (icm) uutgrowths
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2016
url http://journalarticle.ukm.my/9746/
http://journalarticle.ukm.my/9746/
http://journalarticle.ukm.my/9746/1/09_Asdiana_Amri.pdf
first_indexed 2023-09-18T19:55:35Z
last_indexed 2023-09-18T19:55:35Z
_version_ 1777406507082579968

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