Bioactivity and stability studies of anthocyanin-containing extracts from Garcinia mangostana L. and Etlingera elatior Jack

Anthocyanin-containing extract (ACE) were prepared from the pericarp of Garcinia mangostana L. (mangosteen) and the inflorescence part of Etlingera elatior Jack (torch ginger) by using acidified methanol as extraction solvent. Our present study focuses on the antioxidant and cytotoxic activity and t...

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Main Authors:	Sujatha Ramasamy, Nor Afifa Mazlan, Nurul Asyikin Ramli, Wan Nur Asyiqin Rasidi, Sugumaran Manickam
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2016
Online Access:	http://journalarticle.ukm.my/9744/ http://journalarticle.ukm.my/9744/ http://journalarticle.ukm.my/9744/1/07_Sujatha_Ramasamy.pdf

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recordtype	eprints
spelling	ukm-97442016-12-14T06:50:42Z http://journalarticle.ukm.my/9744/ Bioactivity and stability studies of anthocyanin-containing extracts from Garcinia mangostana L. and Etlingera elatior Jack Sujatha Ramasamy, Nor Afifa Mazlan, Nurul Asyikin Ramli, Wan Nur Asyiqin Rasidi, Sugumaran Manickam, Anthocyanin-containing extract (ACE) were prepared from the pericarp of Garcinia mangostana L. (mangosteen) and the inflorescence part of Etlingera elatior Jack (torch ginger) by using acidified methanol as extraction solvent. Our present study focuses on the antioxidant and cytotoxic activity and the effect of temperature, light and pH on stability of ACE derived from G. mangostana and E. elatior. The monomeric anthocyanin and total phenolic content in E. elatior was higher (43.42±0.01 mg/L dan 10.07±0.01 gGAE /100 g) compared to G. mangostana (15.03±0.01 mg/L and 3.29±0.01 gGAE/100 g). Both ACE of E. elatior and G. mangostana exhibited free radical scavenger activity, with IC50 value of14.90±1.02 mg/mL and 15.50±0.52 mg/mL, respectively, in 2,2’-diphenyl-1-picrylhydrazyl assay. ACE of E. elatior was also found to be a good reducing and possessed higher absorbance values at concentrations range from 1.25 to 20 mg/mL in FRAP assay. ACE of E. elatior exhibited mild cytotoxicity on human ovarian SKOV-3 cell line with IC50 values of 54.32 ± 4.60 μg/mL. The effects of light and temperature on ACE stability were performed in different environmental conditions, which promote the destabilization of anthocyanin molecules. ACE stability of G. mangostana were less resist to the effect of light but very susceptible to the prolonged effect of heat after 2 h exposure compared to the ACE in E. elatior. The different in pH highly influence the stability of both ACE which can be observed from the decrease in absorbance readings. Penerbit Universiti Kebangsaan Malaysia 2016-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9744/1/07_Sujatha_Ramasamy.pdf Sujatha Ramasamy, and Nor Afifa Mazlan, and Nurul Asyikin Ramli, and Wan Nur Asyiqin Rasidi, and Sugumaran Manickam, (2016) Bioactivity and stability studies of anthocyanin-containing extracts from Garcinia mangostana L. and Etlingera elatior Jack. Sains Malaysiana, 45 (4). pp. 559-565. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid45bil4_2016/KandunganJilid45Bil4_2016.htm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Anthocyanin-containing extract (ACE) were prepared from the pericarp of Garcinia mangostana L. (mangosteen) and the inflorescence part of Etlingera elatior Jack (torch ginger) by using acidified methanol as extraction solvent. Our present study focuses on the antioxidant and cytotoxic activity and the effect of temperature, light and pH on stability of ACE derived from G. mangostana and E. elatior. The monomeric anthocyanin and total phenolic content in E. elatior was higher (43.42±0.01 mg/L dan 10.07±0.01 gGAE /100 g) compared to G. mangostana (15.03±0.01 mg/L and 3.29±0.01 gGAE/100 g). Both ACE of E. elatior and G. mangostana exhibited free radical scavenger activity, with IC50 value of14.90±1.02 mg/mL and 15.50±0.52 mg/mL, respectively, in 2,2’-diphenyl-1-picrylhydrazyl assay. ACE of E. elatior was also found to be a good reducing and possessed higher absorbance values at concentrations range from 1.25 to 20 mg/mL in FRAP assay. ACE of E. elatior exhibited mild cytotoxicity on human ovarian SKOV-3 cell line with IC50 values of 54.32 ± 4.60 μg/mL. The effects of light and temperature on ACE stability were performed in different environmental conditions, which promote the destabilization of anthocyanin molecules. ACE stability of G. mangostana were less resist to the effect of light but very susceptible to the prolonged effect of heat after 2 h exposure compared to the ACE in E. elatior. The different in pH highly influence the stability of both ACE which can be observed from the decrease in absorbance readings.
format	Article
author	Sujatha Ramasamy, Nor Afifa Mazlan, Nurul Asyikin Ramli, Wan Nur Asyiqin Rasidi, Sugumaran Manickam,
spellingShingle	Sujatha Ramasamy, Nor Afifa Mazlan, Nurul Asyikin Ramli, Wan Nur Asyiqin Rasidi, Sugumaran Manickam, Bioactivity and stability studies of anthocyanin-containing extracts from Garcinia mangostana L. and Etlingera elatior Jack
author_facet	Sujatha Ramasamy, Nor Afifa Mazlan, Nurul Asyikin Ramli, Wan Nur Asyiqin Rasidi, Sugumaran Manickam,
author_sort	Sujatha Ramasamy,
title	Bioactivity and stability studies of anthocyanin-containing extracts from Garcinia mangostana L. and Etlingera elatior Jack
title_short	Bioactivity and stability studies of anthocyanin-containing extracts from Garcinia mangostana L. and Etlingera elatior Jack
title_full	Bioactivity and stability studies of anthocyanin-containing extracts from Garcinia mangostana L. and Etlingera elatior Jack
title_fullStr	Bioactivity and stability studies of anthocyanin-containing extracts from Garcinia mangostana L. and Etlingera elatior Jack
title_full_unstemmed	Bioactivity and stability studies of anthocyanin-containing extracts from Garcinia mangostana L. and Etlingera elatior Jack
title_sort	bioactivity and stability studies of anthocyanin-containing extracts from garcinia mangostana l. and etlingera elatior jack
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2016
url	http://journalarticle.ukm.my/9744/ http://journalarticle.ukm.my/9744/ http://journalarticle.ukm.my/9744/1/07_Sujatha_Ramasamy.pdf
first_indexed	2023-09-18T19:55:35Z
last_indexed	2023-09-18T19:55:35Z
_version_	1777406506774298624

Bioactivity and stability studies of anthocyanin-containing extracts from Garcinia mangostana L. and Etlingera elatior Jack

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