Studies on separation and properties of lumbrokinase in pheretima praepinguis

In order to separate lumbrokinase in Pheretima praepinguis and to study the enzymatic properties, with Pheretima praepinguis as material, lumbrokinase was separated with the salting out method. Lumbrokinase activity was measured with casein medium plate method. The effect of pH and temperature on lu...

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Main Authors: Tang, Mei, Liu, Cao, Liang, Zi, Gong, Mingfu, Hu, Die
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2016
Online Access:http://journalarticle.ukm.my/9576/
http://journalarticle.ukm.my/9576/
http://journalarticle.ukm.my/9576/1/14_Tang_Mei.pdf
id ukm-9576
recordtype eprints
spelling ukm-95762016-12-14T06:50:21Z http://journalarticle.ukm.my/9576/ Studies on separation and properties of lumbrokinase in pheretima praepinguis Tang, Mei Liu, Cao Liang, Zi Gong, Mingfu Hu, Die In order to separate lumbrokinase in Pheretima praepinguis and to study the enzymatic properties, with Pheretima praepinguis as material, lumbrokinase was separated with the salting out method. Lumbrokinase activity was measured with casein medium plate method. The effect of pH and temperature on lumbrokinase activity was studied. The activity of lumbrokinase separated from Pheretima praepinguis was relatively high. Lumbrokinase activity in neutral or slightly alkaline environment was higher. Lumbrokinase had tolerance ability to high temperature, with highly enzymatic activity under 60° and wide range of temperature adaptation. Universiti Kebangsaan Malaysia 2016-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9576/1/14_Tang_Mei.pdf Tang, Mei and Liu, Cao and Liang, Zi and Gong, Mingfu and Hu, Die (2016) Studies on separation and properties of lumbrokinase in pheretima praepinguis. Sains Malaysiana, 45 (1). pp. 115-118. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid45bil1_2016/KandunganJilid45Bil1_2016.htm
repository_type Digital Repository
institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description In order to separate lumbrokinase in Pheretima praepinguis and to study the enzymatic properties, with Pheretima praepinguis as material, lumbrokinase was separated with the salting out method. Lumbrokinase activity was measured with casein medium plate method. The effect of pH and temperature on lumbrokinase activity was studied. The activity of lumbrokinase separated from Pheretima praepinguis was relatively high. Lumbrokinase activity in neutral or slightly alkaline environment was higher. Lumbrokinase had tolerance ability to high temperature, with highly enzymatic activity under 60° and wide range of temperature adaptation.
format Article
author Tang, Mei
Liu, Cao
Liang, Zi
Gong, Mingfu
Hu, Die
spellingShingle Tang, Mei
Liu, Cao
Liang, Zi
Gong, Mingfu
Hu, Die
Studies on separation and properties of lumbrokinase in pheretima praepinguis
author_facet Tang, Mei
Liu, Cao
Liang, Zi
Gong, Mingfu
Hu, Die
author_sort Tang, Mei
title Studies on separation and properties of lumbrokinase in pheretima praepinguis
title_short Studies on separation and properties of lumbrokinase in pheretima praepinguis
title_full Studies on separation and properties of lumbrokinase in pheretima praepinguis
title_fullStr Studies on separation and properties of lumbrokinase in pheretima praepinguis
title_full_unstemmed Studies on separation and properties of lumbrokinase in pheretima praepinguis
title_sort studies on separation and properties of lumbrokinase in pheretima praepinguis
publisher Universiti Kebangsaan Malaysia
publishDate 2016
url http://journalarticle.ukm.my/9576/
http://journalarticle.ukm.my/9576/
http://journalarticle.ukm.my/9576/1/14_Tang_Mei.pdf
first_indexed 2023-09-18T19:55:16Z
last_indexed 2023-09-18T19:55:16Z
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