Development of the compact UV illuminator with single UV lamp

This paper presents a low-cost method of constructing the compact UV illuminator, which is considered as an important component of a gel documentation system. The procedure involves using a smallest-possible UV lamp and a motor which moves the UV lamp in the UV illuminator instead of conventional 4...

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Main Authors: Jong, Dae Kim, Deuk, Ju Lee, Sang, Yoon Kim, Yu, Seop Kim, Hye, Jeong Song, Chan, Young Park
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2015
Online Access:http://journalarticle.ukm.my/9490/
http://journalarticle.ukm.my/9490/
http://journalarticle.ukm.my/9490/1/06_Jong-Dae_Kim.pdf
id ukm-9490
recordtype eprints
spelling ukm-94902016-12-14T06:50:05Z http://journalarticle.ukm.my/9490/ Development of the compact UV illuminator with single UV lamp Jong, Dae Kim Deuk, Ju Lee Sang, Yoon Kim Yu, Seop Kim Hye, Jeong Song Chan, Young Park This paper presents a low-cost method of constructing the compact UV illuminator, which is considered as an important component of a gel documentation system. The procedure involves using a smallest-possible UV lamp and a motor which moves the UV lamp in the UV illuminator instead of conventional 4 UV lamps. A comparative analysis of images produced by using the commercial gel documentation system and our prototype was carried out. These comparisons were done in real DNA gel as well as a reference plate made of quantum dot. The plate was composed of the chambers filled with various densities of the quantum dot instead of the Agarose gel containing the ETBR in order to increase the accuracy of comparison and the convenience of experiments. Despite the use of only 1 UV lamp, the proposed system demonstrated a similar imaging performance compared with the conventional gel documentation system equipped with 4 UV lamps, resulting in the great reduction of the system cost. Universiti Kebangsaan Malaysia 2015-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9490/1/06_Jong-Dae_Kim.pdf Jong, Dae Kim and Deuk, Ju Lee and Sang, Yoon Kim and Yu, Seop Kim and Hye, Jeong Song and Chan, Young Park (2015) Development of the compact UV illuminator with single UV lamp. Sains Malaysiana, 44 (12). pp. 1693-1699. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol44num12_2015/contentsVol44num12_2015.html
repository_type Digital Repository
institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description This paper presents a low-cost method of constructing the compact UV illuminator, which is considered as an important component of a gel documentation system. The procedure involves using a smallest-possible UV lamp and a motor which moves the UV lamp in the UV illuminator instead of conventional 4 UV lamps. A comparative analysis of images produced by using the commercial gel documentation system and our prototype was carried out. These comparisons were done in real DNA gel as well as a reference plate made of quantum dot. The plate was composed of the chambers filled with various densities of the quantum dot instead of the Agarose gel containing the ETBR in order to increase the accuracy of comparison and the convenience of experiments. Despite the use of only 1 UV lamp, the proposed system demonstrated a similar imaging performance compared with the conventional gel documentation system equipped with 4 UV lamps, resulting in the great reduction of the system cost.
format Article
author Jong, Dae Kim
Deuk, Ju Lee
Sang, Yoon Kim
Yu, Seop Kim
Hye, Jeong Song
Chan, Young Park
spellingShingle Jong, Dae Kim
Deuk, Ju Lee
Sang, Yoon Kim
Yu, Seop Kim
Hye, Jeong Song
Chan, Young Park
Development of the compact UV illuminator with single UV lamp
author_facet Jong, Dae Kim
Deuk, Ju Lee
Sang, Yoon Kim
Yu, Seop Kim
Hye, Jeong Song
Chan, Young Park
author_sort Jong, Dae Kim
title Development of the compact UV illuminator with single UV lamp
title_short Development of the compact UV illuminator with single UV lamp
title_full Development of the compact UV illuminator with single UV lamp
title_fullStr Development of the compact UV illuminator with single UV lamp
title_full_unstemmed Development of the compact UV illuminator with single UV lamp
title_sort development of the compact uv illuminator with single uv lamp
publisher Universiti Kebangsaan Malaysia
publishDate 2015
url http://journalarticle.ukm.my/9490/
http://journalarticle.ukm.my/9490/
http://journalarticle.ukm.my/9490/1/06_Jong-Dae_Kim.pdf
first_indexed 2023-09-18T19:55:02Z
last_indexed 2023-09-18T19:55:02Z
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