Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia

Microbial communities of two oil reservoirs from Malaysia, denoted as Platform Bo and Platform Pe were studied using culture-independent approach. Environmental DNA was extracted and the universal amplified ribosomal region (UARR) was target amplified for both prokaryotes and eukaryotes. The amplifi...

Full description

Bibliographic Details
Main Authors:	Cheng, Yee Fish-Low, Ainon Hamzah, Hui, Yee Chee
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/9372/ http://journalarticle.ukm.my/9372/ http://journalarticle.ukm.my/9372/1/13_Cheng-Yee_Fish.pdf

id	ukm-9372
recordtype	eprints
spelling	ukm-93722016-12-14T06:49:44Z http://journalarticle.ukm.my/9372/ Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia Cheng, Yee Fish-Low Ainon Hamzah, Hui, Yee Chee Microbial communities of two oil reservoirs from Malaysia, denoted as Platform Bo and Platform Pe were studied using culture-independent approach. Environmental DNA was extracted and the universal amplified ribosomal region (UARR) was target amplified for both prokaryotes and eukaryotes. The amplified products were purified and cloned into pTZ57R/T vector to construct the 16S/18S rDNA library. Restriction endocucleases HhaI and MspI were used to screen the library. From that, 125 and 253 recombinant plasmid representative clones from Platform Bo and Platform Pe, respectively, were sent for DNA sequencing. Twenty-six operational taxonomic units (OTUs) consist of 20 genera detected at Platform Bo and 17 OTUs consist of 13 genera detected at Platform Pe. Marinobacter and Acinetobacter species co-occurred in both platforms whereas the rest are site-specific. Gammaproteobacteria accounted for 86.0% of the microbial community in Platform Bo, where OTUs affiliated to Marinobacter, Pseudomonas and Marinobacterium that were the most abundant. The major OTUs in the Platform Pe were with affinities to Achromobacter, followed by Stenotrophomonas and Serratia. The only archaeal isolates were detected in Platform Pe, which affiliated to Thermocladium. The singletons and doubletons accounted for about 50.0% of the OTU abundance in both platforms, which considerably significant despite their rare occurrence. Universiti Kebangsaan Malaysia 2015-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9372/1/13_Cheng-Yee_Fish.pdf Cheng, Yee Fish-Low and Ainon Hamzah, and Hui, Yee Chee (2015) Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia. Sains Malaysiana, 44 (11). pp. 1625-1633. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid44bil11_2015/KandunganJilid44Bil11_2015.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Microbial communities of two oil reservoirs from Malaysia, denoted as Platform Bo and Platform Pe were studied using culture-independent approach. Environmental DNA was extracted and the universal amplified ribosomal region (UARR) was target amplified for both prokaryotes and eukaryotes. The amplified products were purified and cloned into pTZ57R/T vector to construct the 16S/18S rDNA library. Restriction endocucleases HhaI and MspI were used to screen the library. From that, 125 and 253 recombinant plasmid representative clones from Platform Bo and Platform Pe, respectively, were sent for DNA sequencing. Twenty-six operational taxonomic units (OTUs) consist of 20 genera detected at Platform Bo and 17 OTUs consist of 13 genera detected at Platform Pe. Marinobacter and Acinetobacter species co-occurred in both platforms whereas the rest are site-specific. Gammaproteobacteria accounted for 86.0% of the microbial community in Platform Bo, where OTUs affiliated to Marinobacter, Pseudomonas and Marinobacterium that were the most abundant. The major OTUs in the Platform Pe were with affinities to Achromobacter, followed by Stenotrophomonas and Serratia. The only archaeal isolates were detected in Platform Pe, which affiliated to Thermocladium. The singletons and doubletons accounted for about 50.0% of the OTU abundance in both platforms, which considerably significant despite their rare occurrence.
format	Article
author	Cheng, Yee Fish-Low Ainon Hamzah, Hui, Yee Chee
spellingShingle	Cheng, Yee Fish-Low Ainon Hamzah, Hui, Yee Chee Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
author_facet	Cheng, Yee Fish-Low Ainon Hamzah, Hui, Yee Chee
author_sort	Cheng, Yee Fish-Low
title	Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
title_short	Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
title_full	Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
title_fullStr	Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
title_full_unstemmed	Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
title_sort	culture-independent-based analyses identified site-specific microbial in two oil-wells from malaysia
publisher	Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/9372/ http://journalarticle.ukm.my/9372/ http://journalarticle.ukm.my/9372/1/13_Cheng-Yee_Fish.pdf
first_indexed	2023-09-18T19:54:41Z
last_indexed	2023-09-18T19:54:41Z
_version_	1777406449921556480

Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia

Similar Items